LEADER 04501oam 2200625I 450 001 9910461354103321 005 20200520144314.0 010 $a0-429-16573-0 010 $a1-4398-4832-7 024 7 $a10.1201/b10919 035 $a(CKB)2670000000093650 035 $a(EBL)726865 035 $a(OCoLC)730914422 035 $a(SSID)ssj0000522832 035 $a(PQKBManifestationID)11320624 035 $a(PQKBTitleCode)TC0000522832 035 $a(PQKBWorkID)10538804 035 $a(PQKB)11536211 035 $a(MiAaPQ)EBC726865 035 $a(Au-PeEL)EBL726865 035 $a(CaPaEBR)ebr10473076 035 $a(OCoLC)741123114 035 $a(EXLCZ)992670000000093650 100 $a20180331d2011 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aGene biotechnology /$fWilliam Wu. [et al.] 205 $a3rd ed. 210 1$aBoca Raton :$cCRC Press,$d2011. 215 $a1 online resource (562 p.) 300 $aA CRC title. 311 $a1-4398-4830-0 320 $aIncludes bibliographical references. 327 $aFront Cover; Contents; Preface; Authors; Chapter 1: Strategies for Novel Research Projects and/or Research Grant Funding; Chapter 2: Rapid Isolation of Specific cDNAs or Genes by PCR; Chapter 3: Construction and Screening of Subtracted and Complete Expression cDNA Libraries; Chapter 4: Subcloning of Genes or DNA Fragments; Chapter 5: Nonisotopic and Isotopic DNA or RNA Sequencing; Chapter 6: Bioinformation Superhighway and Computer Databases of Nucleic Acids and Proteins; Chapter 7: Characterization of DNA or Genes by Southern Blot Hybridization 327 $aChapter 8: Gene Overexpression by Sense RNA in Mammalian SystemsChapter 9: Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies; Chapter 10: Analysis of Gene Expression at Functional Genomic Level Using Northern Blotting or PCR; Chapter 11: Analysis of Gene Expression at Proteomic Level via Western Blotting; Chapter 12: Analysis of Cellular DNA or Abundance of mRNA by Radioactivity In Situ Hybridization; Chapter 13: Localization of DNA or Abundance of mRNA by Fluorescence In Situ Hybridization 327 $aChapter 14: In Situ PCR Hybridization of Low Copy Genes and in Situ RT-PCR Detection of Low Abundance mRNAsChapter 15: Isolation and Characterization of Genes from Genomic DNA Libraries; Chapter 16: Mouse Stem Cells as a Model Mammalian Cell Line for Gene Expression; Chapter 17: Strategies for Gene Double Knockout; Chapter 18: Large-Scale Expression and Purification of Recombinant Proteins in Cultured Cells; Chapter 19: Quantitative Analysis of Functional Genome by Real-Time RT-PCR; Chapter 20: High-Throughput Analysis of Gene Expression by Cutting-Edge Technology-DNA Microarrays (Gene Chips) 327 $aChapter 21: Construction and Screening of Human Antibody Libraries Using Phage Display TechnologyChapter 22: Down-Regulation of Gene Expression in Mammalian Systems via siRNA Technology; Chapter 23: Strategies for Gene Cloning, Expression, and Identification of Protein-Protein Interaction; Chapter 24: Conditional Gene Knockout; Chapter 25: How to Write a Research Manuscript for Publication in an English Journal; Chapter 26: How to Protect Your Discovery and Invention : Patent 101; Chapter 27: Determination of Transgene Copy Numbers and Practical Biocalculation; Back Cover 330 $aThe third edition of this popular reference covers a variety of techniques related to gene manipulation, including DNA isolation, preparation, screening, and analysis. Topics range from very basic methods to current and sophisticated technologies, including methodologies created and tested by the authors. Other topics include approaches to grant funding and SiRNA technology. The authors offer detailed, step-by-step explanations of protocols and helpful troubleshooting guides. This edition features new techniques for every chapter, as well as several new chapters--Provided by publisher. 606 $aGenetic engineering$vLaboratory manuals 606 $aMolecular biology$vLaboratory manuals 608 $aElectronic books. 615 0$aGenetic engineering 615 0$aMolecular biology 676 $a660.6/5078 700 $aWu$b William.$0737012 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910461354103321 996 $aGene biotechnology$92101266 997 $aUNINA