LEADER 04349oam 2200661I 450 001 9910458373803321 005 20200520144314.0 010 $a0-203-37736-2 010 $a0-203-36060-5 010 $a9786610037223 010 $a1-134-69983-2 010 $a1-280-03722-9 024 7 $a10.4324/9780203360606 035 $a(CKB)1000000000398625 035 $a(StDuBDS)AH3707520 035 $a(SSID)ssj0000280203 035 $a(PQKBManifestationID)11251374 035 $a(PQKBTitleCode)TC0000280203 035 $a(PQKBWorkID)10268930 035 $a(PQKB)10399496 035 $a(MiAaPQ)EBC169956 035 $a(Au-PeEL)EBL169956 035 $a(CaPaEBR)ebr10061127 035 $a(CaONFJC)MIL3722 035 $a(OCoLC)437078633 035 $a(OCoLC)560455920 035 $a(EXLCZ)991000000000398625 100 $a20180331d1999 uy 0 101 0 $aeng 135 $aur||||||||||| 181 $ctxt 182 $cc 183 $acr 200 00$aHeart and soul $ethe therapeutic face of philosophy /$fedited by Chris Mace 210 1$aLondon ;$aNew York :$cRoutledge,$d1999. 215 $a1 online resource (203 p. ) $ccol. ill 300 $aOriginally published: 2003. 300 $aFormerly CIP.$5Uk 311 $a0-415-17000-1 311 $a0-415-17001-X 320 $aIncludes bibliographical references and index. 327 $tchapter 1 Introduction -- $tPhilosophy and psychotherapy /$rChris Mace -- $tpart 2 Therapeutic questioning and Socratic dialogue /$rCHRIS MACE -- $tchapter 2 Therapeutic questioning and Socratic dialogue /$rChris Mace -- $tpart 3 The will in the light of object relations theory /$rPAUL STURDEE -- $tchapter 3 The will in the light of object relations theory /$rPaul Sturdee -- $tpart 4 Scepticism and psychotherapy: a Wittgensteinian approach /$rJOHN M.HEATON -- $tchapter 4 Scepticism and psychotherapy: A Wittgensteinian approach /$rJohn M.Heaton -- $tpart 5 Picture this: Wittgenstein and personal construct theory /$rJOADY BRENNAN -- $tchapter 5 Picture this Wittgenstein and personal construct theory /$rJoady Brennan -- $tpart 6 I or me??: the logic of human relations /$rWERDIE VAN STADEN -- $tchapter 6 I or me The logic of human relations /$rWerdie van Staden -- $tpart 7 The dialogical heart of intersubjectivity /$rJOHN WHEWAY -- $tchapter 7 The dialogical heart of intersubjectivity /$rJohn Wheway -- $tpart 8 Can meanings be causes? /$rDIGBY TANTAM -- $tchapter 8 Can meanings be causes? /$rDigby Tantam -- $tpart 9 Narrative, attachment and the therapeutic process /$rJEREMY HOLMES -- $tchapter 9 Narrative, attachment and the therapeutic process /$rJeremy Holmes -- $tpart 10 Narrative and interpretation /$rPAUL SEPPING -- $tchapter 10 Narrative and interpretation /$rPaul Sepping -- $tpart 11 Self-reflection and the mirror /$rGERALDINE SHIPTON -- $tchapter 11 Self-reflection and the mirror /$rGeraldine Shipton -- $tpart 12 Seventeen syllables for the self /$rMYRA THOMAS -- $tchapter 12 Seventeen syllables for the self /$rMyra Thomas -- $tpart 13 Existentialism and existential psychotherapy /$rEMMY VAN DEURZEN -- $tchapter 13 Existentialism and existential psychotherapy /$rEmmy van Deurzen -- $tpart 14 Leaping beyond theory /$rJOAN HURD -- $tchapter 14 Leaping beyond theory /$rJoan Hurd -- $tpart 15 Thinking as a healing process /$rCATHERINE WIEDER -- $tchapter 15 Thinking as a healing process /$rCatherine Wieder -- $tchapter 16 Philosophy as psychotherapy /$rChris Mace. 330 $aChef Kylie Kwong branches out from her Chinese culinary heritage to explore the cuisines of Europe, the Mediterranean and North Africa. With the emphasis on intensity of flavour and using the finest produce, she creates dishes with inspired combinations of Western ingredients and Eastern techniques. 606 $aPsychotherapy$xPhilosophy 606 $aPhilosophy$xTherapeutic use 606 $aPsychotherapy$xMoral and ethical aspects 608 $aElectronic books. 615 0$aPsychotherapy$xPhilosophy. 615 0$aPhilosophy$xTherapeutic use. 615 0$aPsychotherapy$xMoral and ethical aspects. 676 $a616.89/14/01 701 $aMace$b Chris$f1956-$0908396 801 0$bFlBoTFG 801 1$bFlBoTFG 906 $aBOOK 912 $a9910458373803321 996 $aHeart and soul$92031657 997 $aUNINA LEADER 05125nam 2200637 450 001 9910460609803321 005 20210209145034.0 010 $a3-527-65052-0 010 $a3-527-65054-7 010 $a3-527-65055-5 035 $a(CKB)3710000000387702 035 $a(EBL)2008065 035 $a(SSID)ssj0001493114 035 $a(PQKBManifestationID)11855095 035 $a(PQKBTitleCode)TC0001493114 035 $a(PQKBWorkID)11509559 035 $a(PQKB)10133727 035 $a(MiAaPQ)EBC2008065 035 $a(EXLCZ)993710000000387702 100 $a20151106h20142014 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aHandbook of RNA biochemistry /$fedited by Roland K. Hartmann [and three others] 205 $aSecond, completely revised and enlarged edition. 210 1$aWeinheim, Germany :$cWiley-VCH,$d2014. 210 4$dİ2014 215 $a1 online resource (1371 p.) 300 $aDescription based upon print version of record. 311 $a3-527-32776-2 320 $aIncludes bibliographical references and index. 327 $aCover; Title Page; Copyright; Contents; Preface; List of Contributors; Part I RNA Synthesis and Detection; Chapter 1 Enzymatic RNA Synthesis Using Bacteriophage T7 RNA Polymerase; 1.1 Introduction; 1.2 Description of Method - T7 Transcription In vitro; 1.2.1 Templates; 1.2.1.1 Strategy (i): Insertion into a Plasmid; 1.2.1.2 Strategy (ii): Direct Use of Templates Generated by PCR; 1.2.1.3 Strategy (iii): Annealing of a T7 Promoter DNA Oligonucleotide to a Single-Stranded Template; 1.2.2 Special Demands on the RNA Product 327 $a1.2.2.1 Homogeneous 5' and 3' Ends, Small RNAs, Functional Groups at the 5' End1.2.2.2 Modified Substrates; 1.3 Transcription Protocols; 1.3.1 Transcription with Unmodified Nucleotides; 1.3.2 Transcription with 2' -Fluoro-Modified Nucleotides; 1.3.3 T7 Transcripts with 5' - Cap Structures; 1.3.4 Purification; 1.4 Troubleshooting; 1.4.1 Low or No Product Yield; 1.5 Rapid Preparation of T7 RNA Polymerase; 1.5.1 Required Material; 1.5.1.1 Medium; 1.5.1.2 Buffers and Solutions; 1.5.1.3 Electrophoresis and Chromatography; 1.5.2 Procedure 327 $a1.5.2.1 Cell Growth, Induction, and Test for Expression of T7 RNAP1.5.2.2 Purification of T7 RNAP; 1.5.3 Notes and Troubleshooting; References; Chapter 2 Production of RNAs with Homogeneous 5' - and 3' -Ends; 2.1 Introduction; 2.2 Description of Approach; 2.2.1 Cis-Cleaving Autocatalytic Ribozyme Cassettes; 2.2.1.1 The 5' -Cassette; 2.2.1.2 The 3' -Cassette; 2.2.1.3 Purification of Released RNA Product and Conversion of End Groups; 2.2.2 Trans-Cleaving Ribozymes for the Generation of Homogeneous 3' Ends; 2.2.3 Further Strategies toward Homogeneous Ends 327 $a2.3 Critical Experimental Steps, Changeable Parameters, Troubleshooting2.3.1 Construction of Cis-Cleaving 5'- and 3'-Cassettes; 2.4 PCR Protocols; 2.5 Potential Problems; References; Chapter 3 RNA Ligation; 3.1 General Introduction; 3.1.1 T4 Polynucleotide Ligases; 3.1.2 Reaction Mechanism; 3.1.3 Advantages of T4 DNA Ligase for RNA Ligation; 3.1.4 Chapter Structure; 3.2 RNA Ligation Using T4 DNA Ligase (T4 Dnl); 3.2.1 Overview of the RNA Ligation Method Using the T4 DNA Ligase (T4 Dnl); 3.2.2 Large-Scale Transcription and Purification of RNAs 327 $a3.2.3 Generating Homogeneous Acceptor 3' -Ends for Ligation3.2.4 Site-Directed Cleavage with RNase H; 3.2.5 Dephosphorylation and Phosphorylation of RNAs; 3.2.6 RNA Ligation; 3.2.7 Troubleshooting; 3.3 Simultaneous Splint Ligation of Five RNA Fragments to Generate RNAs for FRET Experiments; 3.3.1 Introduction; 3.3.2 Construct Design; 3.3.3 Troubleshooting; 3.3.3.1 Low Overall Ligation Efficiency; 3.3.3.2 Undesired Ligation By-products; 3.3.3.3 RNA Degradation; 3.4 T4 RNA Ligase(s); 3.4.1 Introduction; 3.4.2 Mechanism and Substrate Specificity; 3.4.2.1 Early Studies 327 $a3.4.2.2 Substrate Specificity and Reaction Conditions 330 $aThe second edition of a highly acclaimed handbook and ready reference. Unmatched in its breadth and quality, around 100 specialists from all over the world share their up-to-date expertise and experiences, including hundreds of protocols, complete with explanations, and hitherto unpublished troubleshooting hints. They cover all modern techniques for the handling, analysis and modification of RNAs and their complexes with proteins. Throughout, they bear the practising bench scientist in mind, providing quick and reliable access to a plethora of solutions for practical questions of RNA research 606 $aRNA$vHandbooks, manuals, etc 606 $aBiochemistry$vHandbooks, manuals, etc 606 $aBiochemistry 608 $aElectronic books. 615 0$aRNA 615 0$aBiochemistry 615 0$aBiochemistry. 676 $a572.8/8 702 $aHartmann$b Roland K. 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910460609803321 996 $aHandbook of RNA biochemistry$91420155 997 $aUNINA