LEADER 03765oam 2200649I 450 001 9910459023403321 005 20200520144314.0 010 $a0-429-14677-9 010 $a1-4200-7350-8 024 7 $a10.1201/EBK1420073492 035 $a(CKB)2670000000035693 035 $a(EBL)565953 035 $a(OCoLC)660536815 035 $a(SSID)ssj0000410739 035 $a(PQKBManifestationID)11309157 035 $a(PQKBTitleCode)TC0000410739 035 $a(PQKBWorkID)10351518 035 $a(PQKB)11679618 035 $a(MiAaPQ)EBC565953 035 $a(Au-PeEL)EBL565953 035 $a(CaPaEBR)ebr10408382 035 $a(CaONFJC)MIL693918 035 $a(OCoLC)680620466 035 $a(EXLCZ)992670000000035693 100 $a20180331d2010 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$a3D cell-based biosensors in drug discovery programs $emicrotissue engineering for high throughput screening /$fWilliam S. Kisaalita 210 1$aBoca Raton [Fla.] :$cCRC Press,$d2010. 215 $a1 online resource (392 p.) 300 $aDescription based upon print version of record. 311 $a1-4398-5904-3 311 $a1-322-62636-7 311 $a1-4200-7349-4 320 $aIncludes bibliographical references and index. 327 $aFront Cover; Dedication; Contents; Preface; Author; Part I. Introduction; Chapter 1. Biosensors and Bioassays; Chapter 2. Target-Driven Drug Discovery; Part II. 3D versus 2D Cultures; Chapter 3. Comparative Transcriptional Profiling and Proteomics; Chapter 4. Comparative Structure and Function; Part III. Emerging Design Principles; Chapter 5. Chemical Microenvironmental Factors; Chapter 6. Spatial and Temporal Microenvironmental Factors; Chapter 7. Material Physical Property and Force Microenvironmental Factors; Chapter 8. Proteomics as a Promising Tool in the Search for 3D Biomarkers 327 $aChapter 9. Readout Present and Near FutureChapter 10. Ready-to-Use Commercial 3D Plates; Part IV. Technology Deployment Challenges and Opportunities; Chapter 11. Challenges to Adopting 3D Cultures in HTS Programs; Chapter 12. Cases for 3D Cultures in Drug Discovery; Chapter 13. Ideal Case Study Design; Appendix A: Patents for 3D Scaffolds; Appendix B: Current Drug Targets; Appendix C: Popular Cell Lines in Drug Discovery; Appendix D: Stem Cells in Drug Discovery; Back Cover 330 $aThis book is based upon cutting-edge research conducted in the authors lab (Cellular Bioengineering), which over the past decade has developed a number of sophisticated techniques to facilitate use of 3D cell based assays or biosensors. This book uses data from peer-reviewed publications to conclusively justify use of 3D cell cultures in cell-based biosensors (assays) for (HTS). The majority of assays performed in accelerated drug discovery processes are biochemical in nature, but there is a growing demand for live cell-based assays. Unlike biochemical ones, cellular assays are functional approximations of in vivo biological conditions and can provide more biologically relevant information--Provided by publisher. 606 $aPharmaceutical biotechnology 606 $aBiosensors 606 $aHigh throughput screening (Drug development) 608 $aElectronic books. 615 0$aPharmaceutical biotechnology. 615 0$aBiosensors. 615 0$aHigh throughput screening (Drug development) 676 $a615/.19 700 $aKisaalita$b William Ssempa$f1953,$0993114 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910459023403321 996 $a3D cell-based biosensors in drug discovery programs$92274073 997 $aUNINA LEADER 02686nam 22004693 450 001 9910153608503321 005 20230808200627.0 010 $a3-7345-6797-1 035 $a(CKB)3710000000962536 035 $a(MiAaPQ)EBC4753288 035 $a(Au-PeEL)EBL4753288 035 $a(CaPaEBR)ebr11339790 035 $a(OCoLC)972289404 035 $a(Exl-AI)4753288 035 $a(EXLCZ)993710000000962536 100 $a20210901d2016 uy 0 101 0 $ager 135 $aurcnu|||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aLet's talk about fucking Sex 205 $a1st ed. 210 1$aHamburg :$ctredition Verlag,$d2016. 210 4$d©2016. 215 $a1 online resource (75 pages) 311 08$a3-7345-6796-3 327 $aIntro -- Titel -- Impressum -- Inhaltsverzeichnis -- Vorwort -- Selbstbefriedigung -- Der erste Kuss -- "Flaschendrehen" -- Mein erster "richtiger Freund" -- Das erste Mal -- Ein anderer Ort I -- Angst -- Geruchsintensive Traumku?sse -- Die erste große Liebe -- Ein anderer Ort II -- Einsamkeit & -- Schwa?rmerei -- Der Urlaub -- Sex ohne Beziehung -- Eine lange Beziehung -- Das Rollenspiel -- Der Dreier -- Schluss -- Eine U?berraschung -- Die Wohngemeinschaft -- Alles Scheißegal -- Ende gut, alles gut -- Nachwort -- Danksagung -- Hilfestellen. 330 $aThis autobiographical work by Janya Meier, written under a pseudonym, explores the personal and intimate experiences of the author, aiming to shed light on topics of young adulthood, sexuality, and self-discovery. Addressing themes such as bullying, friendship, and the challenges of navigating early relationships, the book provides candid insights into the realities of growing up as a young woman. The author shares her journey with the intent to inform and encourage reflection, particularly for young women, emphasizing resilience and self-worth in the face of societal pressures and personal struggles. The narrative, while based on true events, ensures anonymity for all involved, blending honesty with sensitivity. The book is intended for mature audiences, particularly young women seeking relatable and empowering life lessons.$7Generated by AI. 606 $aYoung women$xPsychology$7Generated by AI 606 $aSelf-esteem in adolescence$7Generated by AI 615 0$aYoung women$xPsychology 615 0$aSelf-esteem in adolescence 676 $a306.70835 700 $aMeier$b Janya$01264238 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910153608503321 996 $aLet's talk about fucking Sex$92963747 997 $aUNINA