LEADER 05256nam 2200661Ia 450 001 9910458899903321 005 20211207161212.0 010 $a1-282-68585-6 010 $a9786612685859 010 $a1-4443-1861-6 035 $a(CKB)2670000000018927 035 $a(EBL)530049 035 $a(OCoLC)630541245 035 $a(SSID)ssj0000418482 035 $a(PQKBManifestationID)11292704 035 $a(PQKBTitleCode)TC0000418482 035 $a(PQKBWorkID)10376742 035 $a(PQKB)11415196 035 $a(MiAaPQ)EBC530049 035 $a(Au-PeEL)EBL530049 035 $a(CaPaEBR)ebr10387072 035 $a(CaONFJC)MIL268585 035 $a(EXLCZ)992670000000018927 100 $a20091001d2010 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aGene cloning and DNA analysis $b[electronic resource]$ean introduction /$fT.A. Brown 205 $a6th ed. 210 $aHoboken $cWiley-Blackwell$d2010 215 $a1 online resource (338 p.) 300 $aDescription based upon print version of record. 311 $a1-4051-8173-7 327 $aGENECLONINGAND DNAANALYSIS; Contents; TO THE SIXTH EDITION Preface to the Sixth Edition; PART IThe Basic Principlesof Gene Cloning andDNA Analysis; Chapter 1Why Gene Cloningand DNA Analysis areImportant; 1.1 The early development of genetics; 1.2 The advent of gene cloning and the polymerasechain reaction; 1.3 What is gene cloning?; 1.4 What is PCR?; 1.5 Why gene cloning and PCR are so important; 1.5.1 Obtaining a pure sample of a gene by cloning; 1.5.2 PCR can also be used to purify a gene; 1.6 How to find your way through this book 327 $aChapter 2Vectors for GeneCloning:Plasmids andBacteriophages2.1 Plasmids; 2.1.1 Size and copy number; 2.1.2 Conjugation and compatibility; 2.1.3 Plasmid classification; 2.1.4 Plasmids in organisms other than bacteria; 2.2 Bacteriophages; 2.2.1 The phage infection cycle; 2.2.2 Lysogenic phages; Gene organization in the ? DNA molecule; The linear and circular forms of ? DNA; M13 - a filamentous phage; 2.2.3 Viruses as cloning vectors for other organisms; Chapter 3Purification of DNAfrom Living Cells; 3.1 Preparation of total cell DNA; 3.1.1 Growing and harvesting a bacterial culture 327 $a3.1.2 Preparation of a cell extract3.1.3 Purification of DNA from a cell extract; Removing contaminants by organic extraction and enzyme digestion; Using ion-exchange chromatography to purify DNA from a cell extract; 3.1.4 Concentration of DNA samples; 3.1.5 Measurement of DNA concentration; 3.1.6 Other methods for the preparation of total cell DNA; 3.2 Preparation of plasmid DNA; 3.2.1 Separation on the basis of size; 3.2.2 Separation on the basis of conformation; Alkaline denaturation; Ethidium bromide-caesium chloride density gradient centrifugation; 3.2.3 Plasmid amplification 327 $a3.3 Preparation of bacteriophage DNA3.3.1 Growth of cultures to obtain a high ? titer; 3.3.2 Preparation of non-lysogenic ? phages; 3.3.3 Collection of phages from an infected culture; 3.3.4 Purification of DNA from ? phage particles; 3.3.5 Purification of M13 DNA causes few problems; Chapter 4Manipulation ofPurified DNA; 4.1 The range of DNA manipulative enzymes; 4.1.1 Nucleases; 4.1.2 Ligases; 4.1.3 Polymerases; 4.1.4 DNA modifying enzymes; 4.2 Enzymes for cutting DNA - restriction endonucleases; 4.2.1 The discovery and function of restriction endonucleases 327 $a4.2.2 Type II restriction endonucleases cut DNA at specificnucleotide sequences4.2.3 Blunt ends and sticky ends; 4.2.4 The frequency of recognition sequences in a DNAmolecule; 4.2.5 Performing a restriction digest in the laboratory; 4.2.6 Analyzing the result of restriction endonucleasecleavage; Separation of molecules by gel electrophoresis; Visualizing DNA molecules in an agarose gel; 4.2.7 Estimation of the sizes of DNA molecules; 4.2.8 Mapping the positions of different restriction sites in aDNA molecule; 4.2.9 Special gel electrophoresis methods for separatinglarger molecules 327 $a4.3 Ligation - joining DNA molecules together 330 $aKnown world-wide as the standard introductory text to this important and exciting area, the sixth edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented four-colour illustrations. In addition to a number of informative changes to the text throughout the book, the final four chapters have been significa 606 $aMolecular cloning 606 $aNucleotide sequence 606 $aDNA$xAnalysis 608 $aElectronic books. 615 0$aMolecular cloning. 615 0$aNucleotide sequence. 615 0$aDNA$xAnalysis. 676 $a572.8/633 700 $aBrown$b T. A$g(Terence A.)$076063 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910458899903321 996 $aGene cloning and DNA analysis$9243337 997 $aUNINA