LEADER 05382nam 2200685Ia 450 001 9910458576203321 005 20200520144314.0 010 $a1-281-05700-2 010 $a9786611057008 010 $a0-08-053177-6 035 $a(CKB)1000000000384291 035 $a(EBL)311334 035 $a(OCoLC)162128918 035 $a(SSID)ssj0000156217 035 $a(PQKBManifestationID)11182665 035 $a(PQKBTitleCode)TC0000156217 035 $a(PQKBWorkID)10122228 035 $a(PQKB)10191658 035 $a(MiAaPQ)EBC311334 035 $a(PPN)182571831 035 $a(Au-PeEL)EBL311334 035 $a(CaPaEBR)ebr10190068 035 $a(CaONFJC)MIL105700 035 $a(EXLCZ)991000000000384291 100 $a19990212d1999 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aFluorescent and luminescent probes for biological activity$b[electronic resource] $ea practical guide to technology for quantitative real-time analysis /$fedited by WT Mason 205 $a2nd ed. 210 $aSan Diego, Calif. $cAcademic Press$dc1999 215 $a1 online resource (697 p.) 225 1 $aBiological techniques 300 $aDescription based upon print version of record. 311 $a0-12-447836-0 320 $aIncludes bibliographical references and index. 327 $aFront Cover; Fluorescent and Luminescent Probes for Biological Activity: A Practical Guide to Technology for Quantitative Real-Time Analysis; Copyright Page; Series Preface; Preface; Contributors; Contents; Part I: Introduction to Fluorescence Microscopy; Chapter One. Fluorescence Microscopy; 1.1 Introduction; 1.2 Microscope design; 1.3 Types of illumination; 1.4 Light sources; 1.5 Filters; 1.6 Objectives and eyepieces; References; Part II: Optical Probes and Their Applications; Chapter Two. Introduction to Fluorescent Probes: Properties, History and Applications; 2.1 Introduction 327 $a2.2 Nature of fluorescence and properties of fluorescent probes2.3 Historical developments; 2.4 Applications of fluorochromes in histology and microbiology; 2.5 Introduction of acridine orange into cell physiology, cytology and cytochemistry; 2.6 General applications of fluorescent probes; Acknowledgements; References; Chapter Three. Intracellular Ion Indicators; 3.1 Introduction; 3.2 General properties of intracellular ion indicators; 3.3 Examples of intracellular ion indicators; 3.4 Conclusions; Acknowledgements; References 327 $aChapter Four. Fluorescent Imaging of Nucleic Acids and Proteins in Gels4.1 Introduction; 4.2 General properties of fluorescent nucleic acid stains; 4.3 Examples of fluorescent nucleic acid gel stains; 4.4 General properties of fluorophore labels used to detect nucleic acids; 4.5 General properties of fluorescent protein gel stains; 4.6 Examples of fluorescent protein gel stains; 4.7 Protein labelling; 4.8 Conclusions; Acknowledgements; References; Part III: Using Optical Probes in Cells - Practicalities, Problems and Pitfalls 327 $aChapter Five. Introducing and Calibrating Fluorescent Probes in Cells and Organelles5.1 Introduction; 5.2 General principles of the loading process; 5.3 General principles of the calibration process; 5.4 Putting principles into practice; Acknowledgements; References; Chapter Six. Electroporation: A Method for Introduction of Non-permeable Molecular Probes; 6.1 Introduction; 6.2 Basic concept of electroporation; 6.3 Electric field generation and monitoring; 6.4 Polarization of the outer membrane; 6.5 Electropore formation and resealing; 6.6 Transmembrane transport 327 $a6.7 Practical considerations of electroporation6.8 Experimental evidence; 6.9 Summary; Acknowledgements; References; Chapter Seven. Imaging Reality: Understanding Maps of Physiological Cell Signals Measured by Fluorescence Microscopy and Digital Imaging; 7.1 Introduction; 7.2 Generic considerations for the use of fluorescent indicators; 7.3 Optimization of fluorescent light detection and background light correction; 7.4 3-D spatial maps of fluorescent signals; Acknowledgements; References; Chapter Eight. Fluorescent Probes in Practice - Potential Artifacts; 8.1 Introduction 327 $a8.2 Photobleaching 330 $aThe use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes.The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emergi 410 0$aBiological techniques series. 606 $aFluorescent probes 606 $aFluorescence 608 $aElectronic books. 615 0$aFluorescent probes. 615 0$aFluorescence. 676 $a570/.28 701 $aMason$b W. T$0971993 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910458576203321 996 $aFluorescent and luminescent probes for biological activity$92210016 997 $aUNINA