LEADER 05268nam 2200673Ia 450 001 9910457975803321 005 20200520144314.0 010 $a0-08-057212-X 010 $a1-281-01960-7 010 $a9786611019600 010 $a0-08-052790-6 035 $a(CKB)1000000000384300 035 $a(EBL)307203 035 $a(OCoLC)162128986 035 $a(SSID)ssj0000111642 035 $a(PQKBManifestationID)11138672 035 $a(PQKBTitleCode)TC0000111642 035 $a(PQKBWorkID)10080787 035 $a(PQKB)11660904 035 $a(MiAaPQ)EBC307203 035 $a(MiAaPQ)EBC535145 035 $a(PPN)182571912 035 $a(Au-PeEL)EBL307203 035 $a(CaPaEBR)ebr10186714 035 $a(EXLCZ)991000000000384300 100 $a19950425d1996 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aBioconjugate techniques$b[electronic resource] /$fGreg T. Hermanson 210 $aSan Diego $cAcademic Press$d1996 215 $a1 online resource (813 p.) 300 $aDescription based upon print version of record. 311 $a0-12-342336-8 311 $a0-12-342335-X 320 $aIncludes bibliographical references and index. 327 $aFront Cover; Bioconjugate Techniques; Copyright Page; Detailed Contents; Preface; Acknowledgments; Part I: Bioconjugate Chemistry; Chapter 1. Functional Targets; 1. Modification of Amino Acids, Peptides, and Proteins; 2. Modification of Sugars, Polysaccharides, and Glycoconjugates; 3. Modification of Nucleic Acids and Oligonucleotides; 4. Creating Specific Functional Groups; 5. Blocking Specific Functional Groups; Chapter 2. The Chemistry of Reactive Groups; 1. Amine-Reactive Chemical Reactions; 2. Thiol-Reactive Chemical Reactions; 3. Carboxylate-Reactive Chemical Reactions 327 $a4. Hydroxyl-Reactive Chemical Reactions5. Aldehyde- and Ketone-Reactive Chemical Reactions; 6. Active Hydrogen-Reactive Chemical Reactions; 7. Photoreactive Chemical Reactions; Part II: Bioconjugate Reagents; Chapter 3. Zero-Length Cross-linkers; 1. Carbodiimides; 2. Woodward's Reagent K; 3. N, N'-Carbonyldiimidazole; 4. Schiff Base Formation and Reductive Amination; Chapter 4. Homobifunctional Cross-linkers; 1. Homobifunctional NHS Esters; 2. Homobifunctional Imidoesters; 3. Homobifunctional Sulfhydryl-Reactive Cross-linkers; 4. Difluorobenzene Derivatives 327 $a5. Homobifunctional Photoreactive Cross-linkers6. Homobifunctional Aldehydes; 7. Bis-epoxides; 8. Homobifuncrional Hydrazides; 9. Bis-diazonium Derivatives; 10. Bis-alkylhalides; Chapter 5. Heterobifunctional Cross-linkers; 1. Amine-Reactive and Sulfhydryl-Reactive Cross-linkers; 2. Carbonyl-Reactive and Sulfhydryl-Reactive Cross-linkers; 3. Amine-Reactive and Photoreactive Cross-linkers; 4. Sulfhydryl-Reactive and Photoreactive Cross-linkers; 5. Carbonyl-Reactive and Photoreactive Cross-linkers; 6. Carboxylate-Reactive and Photoreactive Cross-linkers 327 $a7. Arginine-Reactive and Photoreactive Cross-linkersChapter 6. Trifunctional Cross-linkers; 1. 4-Azido-2-nitrophenylbiocytin-4-nitrophenyl ester; 2. Sulfo-SBED; Chapter 7. Cleavable Reagent Systems; 1. Cleavage of Disulfides by Reduction; 2. Periodate-Cleavable Glycols; 3. Dithionite-Cleavable Diazo Bonds; 4. Hydroxylamine-Cleavable Esters; 5. Base Labile Sulfones; Chapter 8. Tags and Probes; 1. Fluorescent Labels; 2. Bifunctional Chelating Agents and Radioimmunoconjugates; 3. Biotinylation Reagents; 4. lodination Reagents; Part III: Bioconjugate Applications 327 $aChapter 9. Preparation of Hapten-Carrier Immunogen Conjugates1. The Basis of Immunity; 2. Types of Immunogen Carriers; 3. Carbodiimide-Mediated Hapten-Carrier Conjugation; 4. NHS Ester-Mediated Hapten-Carrier Conjugation; 5. NHS Ester-Maleimide Heterobifunctional Cross-linker- Mediated Hapten-Carrier Conjugation; 6. Active-Hydrogen-Mediated Hapten-Carrier Conjugation; 7. Glutaraldehyde-Mediated Hapten-Carrier Conjugation; 8. Reductive-Amination-Mediated Hapten-Carrier Conjugation; Chapter 10. Antibody Modification and Conjugation; 1. Preparation of Antibody-Enzyme Conjugates 327 $a2. Preparation of Labeled Antibodies 330 $aBioconjugate Techniques is the essential guide to the modification and crosslinking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Armed with this i 606 $aBioconjugates$xSynthesis 606 $aBiochemistry 608 $aElectronic books. 615 0$aBioconjugates$xSynthesis. 615 0$aBiochemistry. 676 $a574.19/296 700 $aHermanson$b Greg T$0511906 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910457975803321 996 $aBioconjugate techniques$9763743 997 $aUNINA