LEADER 05153nam 2200685Ia 450 001 9910457970603321 005 20200520144314.0 010 $a1-281-18650-3 010 $a9786611186500 010 $a0-08-052819-8 035 $a(CKB)1000000000384305 035 $a(EBL)331913 035 $a(OCoLC)476132220 035 $a(SSID)ssj0000112459 035 $a(PQKBManifestationID)11129190 035 $a(PQKBTitleCode)TC0000112459 035 $a(PQKBWorkID)10086928 035 $a(PQKB)11664529 035 $a(MiAaPQ)EBC331913 035 $a(PPN)170265250 035 $a(Au-PeEL)EBL331913 035 $a(CaPaEBR)ebr10216739 035 $a(CaONFJC)MIL118650 035 $a(EXLCZ)991000000000384305 100 $a19950908d1996 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aBiotechnology$b[electronic resource] $ea laboratory course /$fJeffrey M. Becker, Guy A. Caldwell, Eve Ann Zachgo 205 $a2nd ed. 210 $aSan Diego $cAcademic Press$dc1996 215 $a1 online resource (283 p.) 300 $aDescription based upon print version of record. 311 $a0-12-084562-8 320 $aIncludes bibliographical references and index. 327 $aFront Cover; Biotechnology: A Laboratory Course; Copyright Page; Contents; Preface to the Second Edition; Preface to the First Edition; Acknowledgments; Suggested Schedule for Exercises; Introductory Notes: Record Keeping and Safety Rules; Format of Student Laboratory Records; The Ten Commandments of Record Keeping; Safety Rules in the Laboratory; Exercise 1. Aseptic Technique and Establishing Pure Cultures: The Streak Plate and Culture Transfer; Exercise 2. Preparation of Culture Media; Exercise 3. The Growth Curve; Exercise 4. Isolation of Plasmid DNA from Escherichia coli: The Mini-Prep 327 $aExercise 5. Purification, Concentration, and Quantitation of DNAExercise 6. Large-Scale Isolation of Plasmid DNA by Column Chromatography; Exercise 7. Amplification of a lacZ Gene Fragment by the Polymerase Chain Reaction; Exercise 8. Restriction Digestion and Agarose Gel Electrophoresis; Exercise 9. Southern Transfer; Exercise 10. Preparation, Purification, and Hybridization of Probe; Exercise 11. Transformation of Saccharomyces cerevisiae; Exercise 12. Isolation of Plasmid from Yeast and Escherichia coil Transformation; Exercise 13. Protein Assays 327 $aExercise 14. Qualitative Assay for b-Galactosidase in Yeast ColoniesExercise 15. Determination of b-Galactosidase in Permeabilized Yeast Cells; Exercise 16. Assay of b-Galactosidase in Cell Extracts; Exercise 17. b-Galactosidase Purification; Exercise 18. Western Blot: Probe of Protein Blot with Antibody to b-Galactosidase; Appendix 1. Alternative Protocols and Experiments; Exercise 1A Isolation and Characterization of Auxotrophic Yeast Mutants; Exercise 2A Measurement of pH; Exercise 3A Use of the Spectrophotometer; Exercise 6A Isolation of Plasmid DNA: The Maxi-Prep 327 $aExercise 10A Colony HybridizationAppendix 2. Buffer Solutions; Appendix 3. Preparation of Buffers and Solutions; Appendix 4. Properties of Some Common Concentrated Acids and Bases; Appendix 5. Use of Micropipettors; Appendix 6. Safe Handling of Microorganisms; Appendix 7. List of Cultures; Appendix 8. Storage of Cultures and DNA; Appendix 9. Sterilization Methods; Appendix 10. Preparation of Stock Solutions for Culture Media; Appendix 11. Growth in Liquid Medium; Appendix 12. Determination of Viable Cells; Appendix 13. Determination of Cell Mass; Appendix 14. Determination of Cell Number 327 $aAppendix 15. Nomenclature of StrainsAppendix 16. Glassware and Plasticware; Appendix 17. Preparation of Tris and EDTA; Appendix 18. Basic Rules for Handling Enzymes; Appendix 19. Effects of Common Contaminants on Protein Assays; Appendix 20. Manufacturers' and Distributors' Addresses; Appendix 21. Surfing the Bionet: World Wide Web Addresses; Glossary; Index 330 $aThe objectives of this Second Edition of Biotechnology: A Laboratory Course remain unchanged: to create a text that consists of a series of laboratory exercises that integrate molecular biology with protein biochemistry techniques while providing a continuum of experiments. The course begins with basic techniques and culminates in the utilization of previously acquired technical experience and experimental material. Two organisms, Sacchaomyces cerevisiae and Escherichia coli, a single plasmid, and a single enzyme are the experimental material, yet the procedures and 606 $aBiochemical engineering 606 $aBiotechnology$vLaboratory manuals 608 $aElectronic books. 615 0$aBiochemical engineering. 615 0$aBiotechnology 676 $a660.6 676 $a660/.6/078 20 700 $aBecker$b Jeffrey M$0621784 701 $aCaldwell$b Guy A$0952474 701 $aZachgo$b Eve Ann$0952475 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910457970603321 996 $aBiotechnology$92153260 997 $aUNINA LEADER 02429oam 2200673 450 001 9910717014403321 005 20230321100758.0 035 $a(CKB)5470000002528066 035 $a(OCoLC)1290897809 035 $a(OCoLC)995470000002528066 035 $a(EXLCZ)995470000002528066 100 $a20220105d2021 ua 0 101 0 $aeng 135 $aur||||||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 13$aAn Act to Ensure That Goods Made With Forced Labor in the Xinjiang Uyghur Autonomous Region of the People's Republic of China Do Not Enter the United States Market, and for Other Purposes 210 1$a[Washington, D.C.] :$c[U.S. Government Publishing Office],$d[2021] 215 $a1 online resource (8 unnumbered pages) 300 $a"Dec. 23, 2021 (H.R. 6256)." 300 $a"135 Stat. 1525." 300 $a"Public Law 117-78." 606 $aForced labor$zChina$zXinjiang Uygur Zizhiqu 606 $aForced labor$xLaw and legislation$zUnited States 606 $aHuman trafficking$zChina$zXinjiang Uygur Zizhiqu 606 $aHuman trafficking$xLaw and legislation$zUnited States 606 $aImports$zUnited States 606 $aForced labor$2fast 606 $aForced labor$xLaw and legislation$2fast 606 $aHuman trafficking$2fast 606 $aHuman trafficking$xLaw and legislation$2fast 606 $aImports$2fast 607 $aChina$zXinjiang Uygur Zizhiqu$2fast 607 $aUnited States$2fast 608 $aStatutes and codes.$2fast 608 $aStatutes and codes.$2lcgft 615 0$aForced labor 615 0$aForced labor$xLaw and legislation 615 0$aHuman trafficking 615 0$aHuman trafficking$xLaw and legislation 615 0$aImports 615 7$aForced labor. 615 7$aForced labor$xLaw and legislation. 615 7$aHuman trafficking. 615 7$aHuman trafficking$xLaw and legislation. 615 7$aImports. 801 0$bGPO 801 1$bGPO 801 2$bGPO 801 2$bOCLCO 801 2$bOCLCF 801 2$bOCLCO 801 2$bAJB 801 2$bGPO 906 $aBOOK 912 $a9910717014403321 996 $aAn Act to Ensure That Goods Made With Forced Labor in the Xinjiang Uyghur Autonomous Region of the People's Republic of China Do Not Enter the United States Market, and for Other Purposes$93536333 997 $aUNINA