LEADER 04640nam 2200661 450 001 9910453519503321 005 20200520144314.0 010 $a3-11-080890-0 024 7 $a10.1515/9783110808902 035 $a(CKB)2550000001178452 035 $a(OCoLC)865508451 035 $a(CaPaEBR)ebrary10819933 035 $a(SSID)ssj0001082726 035 $a(PQKBManifestationID)11667913 035 $a(PQKBTitleCode)TC0001082726 035 $a(PQKBWorkID)11100798 035 $a(PQKB)10254734 035 $a(MiAaPQ)EBC3045163 035 $a(DE-B1597)41161 035 $a(OCoLC)979751760 035 $a(DE-B1597)9783110808902 035 $a(Au-PeEL)EBL3045163 035 $a(CaPaEBR)ebr10819933 035 $a(CaONFJC)MIL558640 035 $a(OCoLC)922948486 035 $a(EXLCZ)992550000001178452 100 $a19950830h19961995 uy| 0 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt 182 $cc 183 $acr 200 00$aCombinatorial libraries $esynthesis, screening and application potential /$feditor, Riccardo Cortese 205 $aReprint 2013 210 1$aNew York :$cWalter de Gruyter,$d1996. 210 4$d©1995 215 $a1 online resource (245 p.) 300 $aBibliographic Level Mode of Issuance: Monograph 311 $a3-11-014395-X 311 $a1-306-27389-7 320 $aIncludes bibliographical references and index. 327 $tFrontmatter -- $tIntroduction -- $tContents -- $tA Synthetic Peptide Libraries -- $t1 Soluble Synthetic Combinatorial Libraries: The Use of Molecular Diversities for Drug Discovery / $rDörner, Barbara / Blondelle, Sylvie E. / Pinilla, Clemencia / Appel, Jon / Dooley, Colette T. / Eichler, Jutta / Ostresh, John M. / Pérez- Payá, Enrique / Houghten, Richard A. -- $t2 Combinatorial Libraries of Synthetic Structures: Synthesis, Screening, and Structure Determination / $rKrchńák, Viktor / Sepetov, Nikolai F. / Kocis, Petr / Patek, Marcel / Lam, Kit S. / Lebl, Michal -- $t3 Peptide Libraries Bound to Continuous Cellulose Membranes: Tools to Study Molecular Recognition / $rSchneider-Mergener, Jens / Kramer, Achim / Reineke, Ulrich -- $t? Nucleic Acids Libraries -- $t4 In Vitro Selection of Nucleic Acid Sequences that Bind Small Molecules / $rLorsch, Jon R. / Szostak, Jack W. -- $t5 Discovery and Characterization of a Thrombin Aptamer Selected from a Combinatorial ssDNA Library / $rGriffin, Linda C. / Leung, Lawrence L.K. -- $tC Phage Display of Peptide Libraries -- $t6 Structural and Functional Constraints in the Display of Peptides on Filamentous Phage Capsids / $rCesareni, Gianni / Minenkova, Olga / Dente, Luciana / Iannolo, Gioacchino / Zucconi, Adriana / Helmer Citterich, Manuela / Lanfrancotti, Alessandra / Castagnoli, Luisa / Vetriani, Costantino -- $t7 Conformationally Defined Peptide Libraries on Phage: Selectable Templates for the Design of Pharmacological Agents / $rSollazzo, Maurizio / Bianchi, Elisabetta / Felici, Franco / Cortese, Riccardo / Pessi, Antonello -- $t8 Discovery of Disease-Specific Mimotopes by Screening Phage Libraries with Human Serum Samples / $rNicosia, Alfredo / Monaci, Paolo / Luzzago, Allessandra / Galfré, Giovanni / Felici, Franco / Prezzi, Caterina / Mennuni, Carmela / Meola, Annalisa / Mecchia, Monica / Cortese, Riccardo -- $t9 The Utilization of Platelets and Whole Cells for the Selection of Peptides Ligands from Phage Display Libraries / $rDoyle, Michael V. / Doyle, Laura V. / Fong, Susan / Goodson, Robert J. / Panganiban, Lootsee / Drummond, Robert / Winter, Jill / Rosenberg, Steven -- $t10 Identification of MHC Binding Motifs with Synthetic and Phage Displayed Peptide Libraries / $rHammer, Juergen / Sinigaglia, Francesco -- $tD Phage Display of Protein Domains -- $t11 Isolating High Affinity Human Antibodies from Phage Repertoires / $rFitzGerald, Kevin / Chiswell, David / Earnshaw, John / Smith, Rodger / Kenten, John / Williams, Richard / McCafferty, John -- $t12 Altering the Function of Enzymes and Macromolecular Inhibitors by Phage Display / $rYang, Qing / Wang, Cheng-I / Craik, Charles S. -- $tAuthors -- $tIndex -- $t Backmatter 606 $aNucleotide sequence 606 $aAmino acid sequence 606 $aGenetic vectors 608 $aElectronic books. 615 0$aNucleotide sequence. 615 0$aAmino acid sequence. 615 0$aGenetic vectors. 676 $a574.87/328 701 $aCortese$b Riccardo$0137780 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910453519503321 996 $aCombinatorial libraries$92474843 997 $aUNINA LEADER 05041nam 22006014a 450 001 9910830383403321 005 20230721030054.0 010 $a1-282-34592-3 010 $a9786612345920 010 $a0-470-05741-6 010 $a0-470-05740-8 035 $a(CKB)1000000000357282 035 $a(EBL)274319 035 $a(OCoLC)162105234 035 $a(SSID)ssj0000302912 035 $a(PQKBManifestationID)11235068 035 $a(PQKBTitleCode)TC0000302912 035 $a(PQKBWorkID)10274411 035 $a(PQKB)10564997 035 $a(MiAaPQ)EBC274319 035 $a(EXLCZ)991000000000357282 100 $a20060823d2007 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aMedical mycology$b[electronic resource] $ecellular and molecular techniques /$fedited by Kevin Kavanagh 210 $aChichester, England ;$aHoboken, NJ $cWiley$dc2007 215 $a1 online resource (350 p.) 300 $aDescription based upon print version of record. 311 $a0-470-01923-9 320 $aIncludes bibliographical references and index. 327 $aMedical Mycology; Contents; Preface; List of Contributors; 1 Diagnosis of Candida Infection in Tissue by Immunohistochemistry; 1.1 Introduction; 1.2 Specificity of monoclonal antibody 3H8 for C. albicans; Protocol 1.1 Testing of specificity of monoclonal antibody 3H8; 1.3 Evaluation of monoclonal antibody 3H8 for the detection of C. albicans morphological forms; Protocol 1.2 Evaluation of monoclonal antibody 3H8 for the detection of C. albicans morphological forms; 1.4 Application of immunohistochemistry in the diagnosis of Candida periodontal disease 327 $aProtocol 1.3 Use of monoclonal antibody 3H8 in the detection of C. albicans in tissue1.5 References; 2 Transmission Electron Microscopy of Pathogenic Fungi; 2.1 Introduction; 2.2 Glutaraldehyde-potassium-permanganate or glutaraldehyde-osmiumtetroxide fixation for ultrastructural analysis; Protocol 2.1 Glutaraldehyde-osmium tetroxide (#) or glutaraldehyde-potassium permanganate (*) fixation for ultrastructural analysis; 2.3 Identification of the different compartments of the secretory pathway in yeasts 327 $aProtocol 2.2 Identification of the different compartments of the secretory pathway in yeasts2.4 Cytochemical localization of acid phosphatase in yeasts; Protocol 2.3 Cytochemical localization of acid phosphatase in yeasts; 2.5 Detection of anionic sites; Protocol 2.4 Detection of anionic sites; 2.6 Detection of glycoconjugates by the periodic acid-thiocarbohydrazide-silver proteinate technique (PATAg); Protocol 2.5 Detection of glycoconjugates by the periodic acid-thiocarbohydrazide-silver proteinate technique (PATAg) 327 $a2.7 Enzyme-gold approach for the detection of polysaccharides in the cell wallProtocol 2.6 Enzyme-gold approach for the detection of polysaccharides in the cell wall; 2.8 Detection of glycoconjugates by the lectin-gold technique; Protocol 2.7 Detection of glycoconjugates by the lectin-gold technique; 2.9 Immunogold detection of antigens on ultrathin sections of acrylic resin; Protocol 2.8 Immunogold detection of antigens on ultrathin sections of acrylic resin; 2.10 Cryofixation and freeze substitution for ultrastructural analysis and immunodetection 327 $aProtocol 2.9 Cryofixation and freeze substitution for ultrastructural analysis and immunodetection2.11 Overview; 2.12 References; 3 Evaluation of Molecular Responses and Antifungal Activity of Phagocytes to Opportunistic Fungi; 3.1 Introduction; 3.2 Preparation of conidia and hyphae of opportunistic fungi; Protocol 3.1 Preparation of conidia and hyphae of opportunistic fungi; Protocol 3.2 Preparation of hyphal fragments; 3.3 Isolation of human monocytes from whole blood; Protocol 3.3 Isolation of human MNCs from whole blood; 3.4 Analysis of human MNC function in response to fungal infection 327 $aProtocol 3.4 XTT microassay 330 $aMedical Mycology: Cellular and Molecular techniques is a clear and concise overview of the subject that details the techniques essential for ongoing research in the area. Drawing together contributions from both scientists and clinicians working in the field, the text will provide a valuable perspective on the applicability of specific techniques to patient care. A wide range of molecular, immunological and cytological techniques are discussed throughout, with the inclusion of protocol section in each chapter designed to provide both a background a up-to-date account of the applicatio 606 $aMedical mycology$vLaboratory manuals 606 $aMycoses$xMolecular aspects$vLaboratory manuals 615 0$aMedical mycology 615 0$aMycoses$xMolecular aspects 676 $a616.96901 701 $aKavanagh$b Kevin$01628178 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910830383403321 996 $aMedical mycology$93965150 997 $aUNINA