LEADER 05195nam 2200637 a 450 001 9910452672903321 005 20200520144314.0 010 $a0-12-382240-8 035 $a(CKB)2550000001106276 035 $a(EBL)1323702 035 $a(OCoLC)854977083 035 $a(SSID)ssj0001085758 035 $a(PQKBManifestationID)11690875 035 $a(PQKBTitleCode)TC0001085758 035 $a(PQKBWorkID)11050982 035 $a(PQKB)11669110 035 $a(MiAaPQ)EBC1323702 035 $a(PPN)176637583 035 $a(Au-PeEL)EBL1323702 035 $a(CaPaEBR)ebr10738880 035 $a(CaONFJC)MIL506634 035 $a(OCoLC)854682481 035 $a(EXLCZ)992550000001106276 100 $a20130814d2013 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aBioconjugate techniques$b[electronic resource]$fGreg T. Hermanson 205 $a3rd ed. 210 $aLondon $cAcademic Press$dc2013 215 $a1 online resource (1165 p.) 300 $aDescription based upon print version of record. 311 $a0-12-382239-4 311 $a1-299-75383-3 320 $aIncludes bibliographical references and index. 327 $aFront Cover; Bioconjugate Techniques; Copyright Page; Dedication; Contents; Preface to the Third Edition; Acknowledgments; Health and Safety; Intellectual Property; Important Information; 1. Introduction to Bioconjugation; 1. What is Bioconjugation?; 2. Bioconjugation Strategy and Design; 2.1. Start with the Application in Mind; 2.2. Designing the Optimal Bioconjugate; 3. The Applications of Bioconjugates; 3.1. Assay and Quantification; Bioconjugates Used in Heterogeneous Immunoassays; Bioconjugates for Enzyme-Linked Immunoassays; Chemiluminescent Acridinium Ester Conjugates 327 $aFluorescent Oligonucleotide ProbesMolecular Scaffolds to Increase Assay Sensitivity; Bioconjugates Used in Homogeneous Assays; Bioconjugates for FRET Assays; Molecular Beacons and TaqMan Probes; Bioluminescence Resonance Energy Transfer (BRET); Protein Fragment Complementation Assays: Split Reporters; Mass Tags for Quantitative Mass Spectrometry; Limiting Nonspecificity in Bioconjugate Designs; 3.2. Detection, Tracking, and Imaging; Fluorescently Labeled Antibodies and Streptavidin; FRET Based Protease Probes; Interacting Proteins or Domains; Probing by Chemoselective Ligation 327 $aProximity Ligation AssaysStaining with Antibody-Enzyme Conjugates; Bioconjugates for Western Blot Detection; Bioconjugates for Super Resolution Microscopy; 3.3. Purification, Capture, and Scavenging; Purification using Immobilized Affinity Ligands; Immunoprecipitation Techniques; Affinity Capture of Post-Translational Modifications; Affinity Capture using Active Site Binding Probes; Affinity Capture of Recombinant Fusion Proteins; Covalent Fusion Tag Technology; Scavenging of Contaminants or Unwanted Components; 3.4. Catalysis and Chemical Modification 327 $aImmobilized Proteases for Proteomic AnalysisImmobilized Reactors in Bioengineering; 3.5. Therapeutics and in vivo Diagnostics; Bioconjugates for Cancer Therapy; Antibody Targeting for Biotherapeutics; Polymeric Scaffolds and Nanoparticles for Biotherapeutic Conjugates; Antibody-Directed Enzyme Prodrug Therapy; Radiolabeled Bioconjugates for Cancer; Boron Neutron Capture Therapy; Photodynamic Therapy; Diagnostic Bioconjugates for In Vivo Imaging; Bioconjugates for Radio Imaging; Bioconjugates for High-Contrast Imaging; NIR Fluorescent Conjugates for In Vivo Imaging 327 $a3.6. Vaccines and Immune ModulationCancer Vaccines; Immunogen Conjugates in the Production of Antibodies; 4. Summary; 2. Functional Targets for Bioconjugation; 1. Modification of Amino Acids, Peptides, and Proteins; 1.1. Protein Structure and Reactivity; Amino Acids; Nucleophilic Reactions and the pI of Amino Acid Side Chains; Secondary, Tertiary, and Quaternary Structure; Prosthetic Groups, Cofactors, and Post-Translational Modifications; Protecting the Native Conformation and Activity of Proteins; Oxidation of Amino Acids in Proteins and Peptides 327 $aSolvent Accessibility of Functional Targets in Proteins 330 $aBioconjugate Techniques, 3rd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions, with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.