LEADER 04871nam 22006855 450 001 9910437827903321 005 20200701155931.0 010 $a1-4614-8666-1 024 7 $a10.1007/978-1-4614-8666-4 035 $a(CKB)3710000000019061 035 $a(EBL)1466681 035 $a(SSID)ssj0001004885 035 $a(PQKBManifestationID)11650432 035 $a(PQKBTitleCode)TC0001004885 035 $a(PQKBWorkID)11046996 035 $a(PQKB)10423019 035 $a(DE-He213)978-1-4614-8666-4 035 $a(MiAaPQ)EBC1466681 035 $a(PPN)172421284 035 $a(EXLCZ)993710000000019061 100 $a20130917d2013 u| 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aApplication of Selected Reaction Monitoring to Highly Multiplexed Targeted Quantitative Proteomics $eA Replacement for Western Blot Analysis /$fby Michael Kinter, Caroline S. Kinter 205 $a1st ed. 2013. 210 1$aNew York, NY :$cSpringer New York :$cImprint: Springer,$d2013. 215 $a1 online resource (76 p.) 225 1 $aSpringerBriefs in Systems Biology,$x2193-4746 300 $aDescription based upon print version of record. 311 $a1-4614-8665-3 320 $aIncludes bibliographical references. 327 $aThe use of mass spectrometry for highly selective detection -- overview of how the selected reaction monitoring experiment works -- Designing a selected reaction monitoring method for a protein -- Example analyses include: sample processing, sample analysis, data processing -- Future Directions. 330 $a  A key experiment in biomedical research is monitoring the expression of different proteins in order to detect changes that occur in biological systems under different experimental conditions.  The method that is most widely used is the Western blot analysis.  While Western blot is a workhorse in laboratories studying protein expression and has several advantages, it also has a number of significant limitations.  In particular, the method is semi-quantitative with limited dynamic range.  Western blot focuses on a single protein per sample with only a small number of representative samples analyzed in an experiment.  New quantitative tools have been needed for some time to at least supplement, & possibly replace, the Western blot. Mass spectrometric methods have begun to compete with Western blot for routine quantitative analyses of proteins.  One of these methods is based on the tandem mass spectrometry technique of selected reaction monitoring (SRM), which is also called multiple reaction monitoring (MRM).  Selected reaction monitoring is actually an older tandem mass spectrometry technique, first described in the late 70s, that is widely utilized in the quantitative analysis of small molecules like drugs & metabolites.  The use of selected reaction monitoring for the quantitative analysis of proteins has a number of advantages.  Most importantly, it is fundamentally quantitative with a wide dynamic range.  The output of the analysis is a numerical result that can range over several orders of magnitude.  Other advantages include sufficient specificity & sensitivity to detect low abundance proteins in complex mixtures.  Finally, selected reaction monitoring can be multiplexed to allow the quantitative analysis of relatively large numbers of proteins in a single sample in a single experiment.     This Brief will explain both the theoretical & experimental details of the selected reaction monitoring experiment as it is applied to proteins. 410 0$aSpringerBriefs in Systems Biology,$x2193-4746 606 $aMedicine 606 $aProteomics 606 $aMass spectrometry 606 $aBiochemistry 606 $aBiomedicine, general$3https://scigraph.springernature.com/ontologies/product-market-codes/B0000X 606 $aProteomics$3https://scigraph.springernature.com/ontologies/product-market-codes/L1403X 606 $aMass Spectrometry$3https://scigraph.springernature.com/ontologies/product-market-codes/C11010 606 $aBiochemistry, general$3https://scigraph.springernature.com/ontologies/product-market-codes/L14005 615 0$aMedicine. 615 0$aProteomics. 615 0$aMass spectrometry. 615 0$aBiochemistry. 615 14$aBiomedicine, general. 615 24$aProteomics. 615 24$aMass Spectrometry. 615 24$aBiochemistry, general. 676 $a610 700 $aKinter$b Michael$4aut$4http://id.loc.gov/vocabulary/relators/aut$0971811 702 $aKinter$b Caroline S$4aut$4http://id.loc.gov/vocabulary/relators/aut 906 $aBOOK 912 $a9910437827903321 996 $aApplication of Selected Reaction Monitoring to Highly Multiplexed Targeted Quantitative Proteomics$92531853 997 $aUNINA