LEADER 04156nam 2200601Ia 450 001 9910437614103321 005 20200520144314.0 010 $a94-007-4531-1 024 7 $a10.1007/978-94-007-4531-5 035 $a(CKB)2560000000091175 035 $a(EBL)971824 035 $a(OCoLC)809767431 035 $a(SSID)ssj0000741879 035 $a(PQKBManifestationID)11473271 035 $a(PQKBTitleCode)TC0000741879 035 $a(PQKBWorkID)10742552 035 $a(PQKB)11052912 035 $a(DE-He213)978-94-007-4531-5 035 $a(MiAaPQ)EBC971824 035 $a(PPN)168338246 035 $a(EXLCZ)992560000000091175 100 $a20120904h20122013 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aSite-directed insertion of transgenes /$fSylvaine Renault, Philippe Duchateau [eitors] 205 $a1st ed. 2013. 210 $aDodrecht ;$aNew York $cSpringer$d2012, c2013 215 $a1 online resource (392 p.) 225 0 $aTopics in current genetics,$x1610-0970 ;$vv.23 300 $aDescription based upon print version of record. 311 $a94-017-8075-7 311 $a94-007-4530-3 320 $aIncludes bibliographical references and index. 327 $aPreface -- Section 1: Site?directed integration of transgenes -- 1 Transgene site-specific integration : troubles and solutions -- 2 :  Designing non-viral targetd integrating vectors for genome enginnering in vertebrates -- 3: Gene targeting and homologous recombination in Saccharomyces cerevisiae4: Eucaryotic homologous recombination in mammalian cells -- 5 Engineered Zinc Finger Nucleases for Targeted Genome Editing -- 6: Engineered meganucleases for genome engineering purposes -- Section3: Integration based on Site-specific recombination -- 7 : Cre/loxP, Flp/FRT systems and pluripotent stem cell lines -- 8 : Site-specific recombination using C31 Integrase -- 9 : Modified transposases used for site-directed insertion of transgenes -- 10 : Targeted Plasmid Integration into the Human Genome by Engineered Recombinases -- 11: Gene site-specific insertion in plants -- 12 Site-directed insertion of transgenes in Drosophila -- 13. Nuclease Mediated Targeted Genome Modification in Mammalian Cells -- 14: Bio-applications derived from site-directed genome modification technologies. 330 $aThe post-genomic era has brought new challenges and opportunities in all fields of the biology. In this context, several genome engineering technologies have emerged that will help deciphering genes function by as well as improve gene therapy strategies. Genomic modifications such as knock-in, knock-out, knock-down, sequence replacement or modification can today be routinely performed. However, in front of this large palette of methodologies scientists may experience difficulties to gather useful information?s scattered within the literature. This book aims to present the state of this field from basic mechanisms of site-directed modifications to their applications in a wide range of organisms such as bacteria, yeast, plants, insects, mammals. It will discuss the problems encountered when using the random integration strategy and present the recent advances made in targeted genome modification. Technologies based on Zinc Finger nucleases, Meganucleases, TALEN, CRE and FLP recombinase, ?C31 integrase, transposases and resolvases are fully detailed with their strengths and weaknesses. All these information?s will help students and experienced researchers to understand and choose the best technology for their own purposes. 410 0$aTopics in Current Genetics,$x1610-2096 ;$v23 606 $aTransgenic animals 606 $aGene targeting$vLaboratory manuals 615 0$aTransgenic animals. 615 0$aGene targeting 676 $a616.02774 701 $aRenault$b Sylvaine$01762499 701 $aDuchateau$b Philippe$01762500 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910437614103321 996 $aSite-directed insertion of transgenes$94202481 997 $aUNINA