LEADER 04570nam 22006375 450 001 9910410043303321 005 20250609110647.0 010 $a981-15-4423-9 024 7 $a10.1007/978-981-15-4423-1 035 $a(CKB)4100000011233807 035 $a(DE-He213)978-981-15-4423-1 035 $a(MiAaPQ)EBC6194050 035 $a(PPN)248392972 035 $a(MiAaPQ)EBC6193618 035 $a(EXLCZ)994100000011233807 100 $a20200509d2020 u| 0 101 0 $aeng 135 $aurnn|008mamaa 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aArtificial Assemblies with Cooperative DNA Recognition /$fby Zutao YU 205 $a1st ed. 2020. 210 1$aSingapore :$cSpringer Singapore :$cImprint: Springer,$d2020. 215 $a1 online resource (XV, 136 p. 136 illus., 119 illus. in color.) 225 1 $aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053 300 $a"Doctoral thesis accepted by Kyoto University, Kyoto, Japan." 311 08$a981-15-4422-0 327 $a1. Synthetic transcription factors (Syn-TFs): design, progress and perspectives -- 2. Pip-HoGu, an artificial assembly with cooperative DNA recognition capable of mimicking transcription factor pairs -- 3. Orthogonal ?PNA dimerization domains empower DNA binders with cooperativity and versatility mimicking that of the transcription factor pairs -- 4. Advanced DNA binding system mimicking the cooperative function of transcription factor pairs precisely recruits the epigenetic modifiers to the DNA repeat binding sites. 330 $aThis book presents three types of synthetically cooperative DNA recognizing assemblies, in order to advance the development of programmable DNA-binding pyrrole?imidazole polyamides (PIPs). PIPs represent the best-characterized class of small molecule DNA binders that can be modified to bind with any predetermined DNA sequence and regulate gene expression patterns in a transgene-free and cost-effective manner. PIPs are characterized by their small molecular size, high binding affinity, programmability, sequence selectivity, and moderate cell permeability. In recent years, there have been numerous novel studies on the applications of these biological tools; this research is thoroughly reviewed in the first chapter. There are several critical issues, however, that impede the further broad study of PIPs, which greatly concern the author. For instance, the short PIP version has an excessively hi^10 bp; this significantly decreases cell permeability. Moreover, the conventional binding strategy for PIP design cannot apply to flexible DNA binding?for example, the DNA-binding mode of a transcription factor pair. In this book, the author describes the development of three kinds of cooperative DNA-binding systems that help resolve the current highly problematic issues concerning PIPs. These three systems offer a range of significant advantages, such as favorable sequence selectivity, long recognition sequence, higher binding affinity, and a flexible gap distance. Released at a critical juncture in the application of PIPs, this book will greatly facilitate their use as therapeutic drugs in the treatment of cancer and hereditary diseases, and in regenerative medicine. . 410 0$aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053 606 $aBiotechnology 606 $aBioorganic chemistry 606 $aNucleic acids 606 $aPharmaceutical chemistry 606 $aBiotechnology$3https://scigraph.springernature.com/ontologies/product-market-codes/C12002 606 $aBioorganic Chemistry$3https://scigraph.springernature.com/ontologies/product-market-codes/C19010 606 $aNucleic Acid Chemistry$3https://scigraph.springernature.com/ontologies/product-market-codes/L14011 606 $aMedicinal Chemistry$3https://scigraph.springernature.com/ontologies/product-market-codes/C28000 615 0$aBiotechnology. 615 0$aBioorganic chemistry. 615 0$aNucleic acids. 615 0$aPharmaceutical chemistry. 615 14$aBiotechnology. 615 24$aBioorganic Chemistry. 615 24$aNucleic Acid Chemistry. 615 24$aMedicinal Chemistry. 676 $a660.6 700 $aYU$b Zutao$4aut$4http://id.loc.gov/vocabulary/relators/aut$01065007 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910410043303321 996 $aArtificial Assemblies with Cooperative DNA Recognition$92542225 997 $aUNINA