LEADER 05758nam 22007695 450 001 9910409688103321 005 20220502163211.0 010 $a981-15-4055-1 024 7 $a10.1007/978-981-15-4055-4 035 $a(CKB)4100000011321555 035 $a(MiAaPQ)EBC6240745 035 $a(DE-He213)978-981-15-4055-4 035 $a(PPN)248600176 035 $a(EXLCZ)994100000011321555 100 $a20200627d2020 u| 0 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aHairy Root Cultures Based Applications $eMethods and Protocols /$fedited by Vikas Srivastava, Shakti Mehrotra, Sonal Mishra 205 $a1st ed. 2020. 210 1$aSingapore :$cSpringer Singapore :$cImprint: Springer,$d2020. 215 $a1 online resource (247 pages) 225 1 $aRhizosphere Biology,$x2523-8442 311 $a981-15-4054-3 327 $aChapter 1. Hairy Roots Biotechnology Unzipped: A Journey of Reality and Promises -- Chapter 2. Hairy Roots as a Source of Tropane Alkaloids -- Chapter 3. Induction, Metabolite Analysis and Transgenesis of Hairy Roots From Coleus Forskohlii -- Chapter 4. Establishment of Hairy Roots of Endangered Himalayan Plantswertiachirata: A Sustainable Alternative to Extraction from Nature -- Chapter 5. A Protocol for the Selection of Spontaneous Variants From Established Catharanthusroseus Hairy Root Cultures -- Chapter 6. Strategies for the Optimization of Culture Conditions for Increasing Metabolite Production Through Hairy Root Cultures. Monoterpenoid Indole Alkaloid Production in Catharanthus Roseus Hairy Roots -- Chapter 7. Protocol for Enhanced Withaferin-a Production in Elicited Withania Somnifera (l.)Dunal Hairy Root Cultures -- Chapter 8. Establishment of Hairy Root Cultures of Pentalinonandrieuxii for the Production of Betulinic Acid -- Chapter 9. Tropane Alkaloid Production by the Establishment Of Hairy Root Cultures of Brugmansia Candida and Elicitation -- Chapter 10. Elicitation as an Essential Strategy For Enhancing Anthraquinone Accumulation In Hairy Root Cultures of Rubia Tinctorum -- Chapter 11. Establishment of Hairy Root Cultures for the Production of Biopharmaceuticals and Optimization of Methods for Recombinant Protein Secretion In The Culture Medium -- Chapter 12. An Improved Protocol for Agrobacterium Rhizogenes-Mediated Transformation of Recalcitrant Plants for Root Biology Studies: A Case Study of Tea Plants (Camellia Sinensis Var. Sinensis) -- Chapter 13 Functional Analysis of Plant Genes Related to Arbuscular Mycorrhiza Symbiosis Using Agrobacterium Rhizogenes-Mediated Root Transformation and Hairy Root Production -- Chapter 14. Functional Characterization of Genes Involved in Legume Nodulation Using Hairy Root Cultures -- Chapter 15. Fluidigm Biomark Diagnostic Panel for Analysis of the Expression of Nicotiana Tabacum Genes, Associated with Alkaloid Synthesis. . 330 $aThe book discusses the various methods and protocols available in hairy root culture-based research. The utilization of Agrobacterium mediated genetic transformation and establishment of hairy root cultures has paved the way for large-scale secondary metabolite production in medicinal plants. Presenting recent research and offering insights from eminent research groups, the book covers a range of topics related to hairy root-based applications, including (i) establishment of hairy roots and native production of SM (ii) yield enhancement strategies for increased SM production, like elicitation (iii) hairy roots as a tool for value-added applications such as plant-microbe interaction, characterization of plant genes and root biology studies. As such it is an informative guide and experimental manual for researchers in diverse fields of plant biology. 410 0$aRhizosphere Biology,$x2523-8442 606 $aBiomedical engineering 606 $aPlant pathology 606 $aMicrobial ecology 606 $aBacteriology 606 $aBiology?Technique 606 $aBiomedical Engineering/Biotechnology$3https://scigraph.springernature.com/ontologies/product-market-codes/B24000 606 $aPlant Pathology$3https://scigraph.springernature.com/ontologies/product-market-codes/L24035 606 $aMicrobial Ecology$3https://scigraph.springernature.com/ontologies/product-market-codes/L19082 606 $aBacteriology$3https://scigraph.springernature.com/ontologies/product-market-codes/L23012 606 $aBiological Techniques$3https://scigraph.springernature.com/ontologies/product-market-codes/L28000 606 $aCultiu de cèl·lules i teixits vegetals$2thub 606 $aPatologia vegetal$2thub 606 $aEnginyeria biomèdica$2thub 608 $aLlibres electrònics$2thub 615 0$aBiomedical engineering. 615 0$aPlant pathology. 615 0$aMicrobial ecology. 615 0$aBacteriology. 615 0$aBiology?Technique. 615 14$aBiomedical Engineering/Biotechnology. 615 24$aPlant Pathology. 615 24$aMicrobial Ecology. 615 24$aBacteriology. 615 24$aBiological Techniques. 615 7$aCultiu de cèl·lules i teixits vegetals 615 7$aPatologia vegetal 615 7$aEnginyeria biomèdica 676 $a575.54 702 $aSrivastava$b Vikas$4edt$4http://id.loc.gov/vocabulary/relators/edt 702 $aMehrotra$b Shakti$4edt$4http://id.loc.gov/vocabulary/relators/edt 702 $aMishra$b Sonal$4edt$4http://id.loc.gov/vocabulary/relators/edt 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910409688103321 996 $aHairy Root Cultures Based Applications$92022558 997 $aUNINA