LEADER 03655nam 22005775 450 001 9910392724103321 005 20200703155037.0 010 $a981-10-4753-7 024 7 $a10.1007/978-981-10-4753-4 035 $a(CKB)4340000000062856 035 $a(DE-He213)978-981-10-4753-4 035 $a(MiAaPQ)EBC4921428 035 $a(PPN)203669649 035 $a(EXLCZ)994340000000062856 100 $a20170720d2018 u| 0 101 0 $aeng 135 $aurnn|008mamaa 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aDevelopment of a Fully Integrated ?Sample-In-Answer-Out? System for Automatic Genetic Analysis /$fby Bin Zhuang 205 $a1st ed. 2018. 210 1$aSingapore :$cSpringer Singapore :$cImprint: Springer,$d2018. 215 $a1 online resource (XXIII, 114 p. 58 illus., 44 illus. in color.) 225 1 $aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053 311 $a981-10-4752-9 320 $aIncludes bibliographical references at the end of each chapters. 327 $aIntroduction -- The construction of a general platform for capillary electrophoresis -- Integrated module for automatic DNA extraction and amplification -- A fully-integrated genetic analysis system -- Conclusion and prospects for future work. 330 $aThis thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an ?in-situ? PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system?s performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successful development and operation of this microsystem establishes the feasibility of rapid ?sample-in-answer-out? testing in routine clinical practice. 410 0$aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053 606 $aBiomedical engineering 606 $aGenetic engineering 606 $aNucleic acids 606 $aBiomedical Engineering and Bioengineering$3https://scigraph.springernature.com/ontologies/product-market-codes/T2700X 606 $aGenetic Engineering$3https://scigraph.springernature.com/ontologies/product-market-codes/C12037 606 $aNucleic Acid Chemistry$3https://scigraph.springernature.com/ontologies/product-market-codes/L14011 615 0$aBiomedical engineering. 615 0$aGenetic engineering. 615 0$aNucleic acids. 615 14$aBiomedical Engineering and Bioengineering. 615 24$aGenetic Engineering. 615 24$aNucleic Acid Chemistry. 676 $a574.873282 700 $aZhuang$b Bin$4aut$4http://id.loc.gov/vocabulary/relators/aut$01064706 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910392724103321 996 $aDevelopment of a Fully Integrated ?Sample-In-Answer-Out? System for Automatic Genetic Analysis$92540276 997 $aUNINA