LEADER 02975nam 2200709z- 450 001 9910372785403321 005 20231214133532.0 010 $a3-03928-023-6 035 $a(CKB)4100000010163772 035 $a(oapen)https://directory.doabooks.org/handle/20.500.12854/42862 035 $a(EXLCZ)994100000010163772 100 $a20202102d2020 |y 0 101 0 $aeng 135 $aurmn|---annan 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aCell-Free Synthetic Biology 210 $cMDPI - Multidisciplinary Digital Publishing Institute$d2020 215 $a1 electronic resource (152 p.) 311 $a3-03928-022-8 330 $aCell-free synthetic biology is in the spotlight as a powerful and rapid approach to characterize and engineer natural biological systems. The open nature of cell-free platforms brings an unprecedented level of control and freedom for design compared to in vivo systems. This versatile engineering toolkit is used for debugging biological networks, constructing artificial cells, screening protein library, prototyping genetic circuits, developing new drugs, producing metabolites, and synthesizing complex proteins including therapeutic proteins, toxic proteins, and novel proteins containing non-standard (unnatural) amino acids. The book consists of a series of reviews, protocols, benchmarks, and research articles describing the current development and applications of cell-free synthetic biology in diverse areas. 610 $agenomically engineered E. coli 610 $ain vitro transcription-translation (TX-TL) 610 $atPa 610 $atissue plasminogen activator 610 $ain vitro translation 610 $aenzymes 610 $aDNA origami 610 $aantimicrobials 610 $acell-free metabolic engineering (CFME) 610 $acell-free transcription-translation 610 $ain vitro protein synthesis 610 $acell-free metabolic engineering 610 $aE. coli crude extract preparation 610 $achaperones 610 $aischemic stroke 610 $amicrosomes 610 $asonication 610 $acell-free protein synthesis 610 $agrowth factors 610 $aartificial cell 610 $aunnatural protein 610 $acell-free synthetic biology 610 $arapid prototyping 610 $adrug development 610 $aunnatural amino acid 610 $aeukaryotic lysates 610 $acell-free protein expression (CFPE) 610 $ariboregulator 610 $asynthetic biology 610 $amathematical model 610 $aCFPS 610 $acell-free protein synthesis (CFPS) 610 $abioconversion platform 610 $acolicins 610 $aprotein production 700 $aHong$b Seok Hoon$4auth$01291812 906 $aBOOK 912 $a9910372785403321 996 $aCell-Free Synthetic Biology$93021952 997 $aUNINA