LEADER 03451nam 2200769z- 450 001 9910367745503321 005 20210211 010 $a3-03921-701-1 035 $a(CKB)4100000010106262 035 $a(oapen)https://directory.doabooks.org/handle/20.500.12854/40355 035 $a(oapen)doab40355 035 $a(EXLCZ)994100000010106262 100 $a20202102d2019 |y 0 101 0 $aeng 135 $aurmn|---annan 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 00$aAdvances in Single Molecule, Real-Time (SMRT) Sequencing 210 $cMDPI - Multidisciplinary Digital Publishing Institute$d2019 215 $a1 online resource (128 p.) 311 08$a3-03921-700-3 330 $aPacBio's single-molecule real-time (SMRT) sequencing technology offers important advantages over the short-read DNA sequencing technologies that currently dominate the market. This includes exceptionally long read lengths (20 kb or more), unparalleled consensus accuracy, and the ability to sequence native, non-amplified DNA molecules. From fungi to insects to humans, long reads are now used to create highly accurate reference genomes by de novo assembly of genomic DNA and to obtain a comprehensive view of transcriptomes through the sequencing of full-length cDNAs. Besides reducing biases, sequencing native DNA also permits the direct measurement of DNA base modifications. Therefore, SMRT sequencing has become an attractive technology in many fields, such as agriculture, basic science, and medical research. The boundaries of SMRT sequencing are continuously being pushed by developments in bioinformatics and sample preparation. This book contains a collection of articles showcasing the latest developments and the breadth of applications enabled by SMRT sequencing technology. 517 $aAdvances in Single Molecule, Real-Time 606 $aBiology, life sciences$2bicssc 610 $aallele-specific analysis 610 $abone marrow cell subpopulations 610 $aCladobotryum protrusum 610 $acobweb disease 610 $acytochrome P450 enzyme (CYP) 610 $ade novo assembly 610 $ade novo genome assembly 610 $aDNA methylation 610 $afull length RNAseq 610 $agene expression 610 $aGloeostereum incarnatum 610 $aGRCh38 610 $ahuman reference genome 610 $ahuman whole-genome sequencing 610 $ainsect genome 610 $along-read SMRT sequencing 610 $alow-input DNA 610 $amosquito 610 $amRNA isoforms 610 $amycoparasite 610 $an/a 610 $anext generation sequencing 610 $aoptical mapping 610 $aPacBio 610 $aPacBio single molecule real-time sequencing 610 $apopulation sequencing 610 $aprotein isoforms 610 $asecondary metabolite 610 $asingle molecule real-time sequencing 610 $aSMRT sequencing 610 $astatistical methods 610 $aSwedish population 610 $aterpenoid 610 $aTricoplusia ni 610 $awhole genome sequencing 615 7$aBiology, life sciences 700 $aAmeur$b Adam$4auth$01290162 702 $aHestand$b Matthew S$4auth 906 $aBOOK 912 $a9910367745503321 996 $aAdvances in Single Molecule, Real-Time (SMRT) Sequencing$93021370 997 $aUNINA