LEADER 04085nam 22005895 450 001 9910367241803321 005 20200704064946.0 010 $a3-030-29646-6 024 7 $a10.1007/978-3-030-29646-9 035 $a(CKB)4100000009382528 035 $a(DE-He213)978-3-030-29646-9 035 $a(MiAaPQ)EBC5940736 035 $a(PPN)24282157X 035 $a(EXLCZ)994100000009382528 100 $a20190930d2020 u| 0 101 0 $aeng 135 $aurnn|008mamaa 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aCatalytically Active Nucleic Acids /$fedited by Harald Seitz, Frank Stahl, Johanna-Gabriela Walter 205 $a1st ed. 2020. 210 1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2020. 215 $a1 online resource (VII, 123 p. 38 illus., 12 illus. in color.) 225 1 $aAdvances in Biochemical Engineering/Biotechnology,$x0724-6145 ;$v170 311 $a3-030-29645-8 327 $aRNA G-Quadruplexes as key Motifs of the Transcriptome -- Challenges and Perspectives in Nucleic Acid Enzyme Engineering -- Strategies for Characterization of Enzymatic Nucleic Acids -- Bioanalytical Application of Peroxidase Mimicking DNAzymes: Status and Challenges -- Hemin/G-Quadruplex Horseradish Peroxidase-Mimicking DNAzyme: Principle and Biosensing Application -- Aptazymes: Expanding the Specificity of Natural Catalytic Nucleic Acids by Application of In Vitro Selected Oligonucleotides. 330 $aThis volume reviews numerous reaction mechanisms and applications of nucleic acids with catalytic activity. Written by an interdisciplinary team of authors, it provides an essential overview of these acids? fundamental aspects, while also addressing associated methodologies such as nucleic acid enzyme engineering, peroxidase-mimicking DNAzymes and Aptazymes. After the discovery of natural ribozymes ? RNA molecules that mediate the cleavage and formation of phosphodiester bonds and the formation of peptide bonds ? numerous artificial ribozymes with altered catalytic activities were produced by in vitro and in vivo selection. Unlike ribozymes, DNAzymes do not occur in nature. Although the catalytic activity of nucleic acid enzymes is usually much slower than that of proteins, nucleic acid enzymes with comparable catalytic activity have been obtained using stringent selection processes. The key advantages of these enzymes: they are e.g. smaller, easier to produce and purify than proteins, and can withstand denaturation, e.g. by heat. Over the last few years, the number of publications on the applications of enzymatic nucleic acids has grown steadily. Summarizing the fundamentals and applications of these acids, this book will not only be an excellent resource for experts in the field but will also guide young researchers just starting out in this significant area. 410 0$aAdvances in Biochemical Engineering/Biotechnology,$x0724-6145 ;$v170 606 $aBiochemical engineering 606 $aNucleic acids 606 $aEnzymology 606 $aBiochemical Engineering$3https://scigraph.springernature.com/ontologies/product-market-codes/C12029 606 $aNucleic Acid Chemistry$3https://scigraph.springernature.com/ontologies/product-market-codes/L14011 606 $aEnzymology$3https://scigraph.springernature.com/ontologies/product-market-codes/L14070 615 0$aBiochemical engineering. 615 0$aNucleic acids. 615 0$aEnzymology. 615 14$aBiochemical Engineering. 615 24$aNucleic Acid Chemistry. 615 24$aEnzymology. 676 $a574.87328 702 $aSeitz$b Harald$4edt$4http://id.loc.gov/vocabulary/relators/edt 702 $aStahl$b Frank$4edt$4http://id.loc.gov/vocabulary/relators/edt 702 $aWalter$b Johanna-Gabriela$4edt$4http://id.loc.gov/vocabulary/relators/edt 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910367241803321 996 $aCatalytically Active Nucleic Acids$92544646 997 $aUNINA