LEADER 05231nam 22006975 450 001 9910350294303321 005 20200702092354.0 010 $a981-13-8094-5 024 7 $a10.1007/978-981-13-8094-5 035 $a(CKB)4100000008876865 035 $a(DE-He213)978-981-13-8094-5 035 $a(MiAaPQ)EBC5776072 035 $a(PPN)236523279 035 $a(EXLCZ)994100000008876865 100 $a20190513d2019 u| 0 101 0 $aeng 135 $aurnn|008mamaa 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aOne-Step Generation of a Drug-Releasing Microarray for High-Throughput Small-Volume Bioassays$b[electronic resource] /$fby Seo Woo Song 205 $a1st ed. 2019. 210 1$aSingapore :$cSpringer Singapore :$cImprint: Springer,$d2019. 215 $a1 online resource (XXI, 52 p. 46 illus., 40 illus. in color.) 225 1 $aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053 311 $a981-13-8093-7 327 $aAbstract -- Table of Contents -- List of Tables -- List of Figures -- Chapter 1. Introduction -- 1.1. High-Throughput Small-Volume Bioassays -- 1.2. Developmental Goal for the ?Pipetting-Free? HTS Platforms -- 1.3. Main Concept: One-Step Generation of a Drug-Releasing Microarray-on-a-Chip by Self-Assembly of Drug-Laden Microparticles (DLPs) -- Chapter 2. System Development -- 2.1. Sealing-Film Assisted Seeding Method for Saving Cell Consumptions -- 2.2. Chip and Jig Development -- 2.3. Preparation of DLPs Library -- 2.4. Decoding Microparticles -- 2.5. Statistical Analysis for Duplications -- Chapter 3. Application: Screening of Sequential Drug Combinations -- 3.1. Therapeutic Benefit of Sequential Drug Combination Based on Rewiring of Intracellular Pathways -- 3.2. Screening of Sequential Drug Combination Using a Partipetting Platform -- 3.3. Proof-of-Concept: Sequential Combinatorial Cell Staining Assay by Replacement of the Drug Chip -- 3.4. Screening of Sequential Combinatorial Drugs with EGFR Inhibitor Followed by Genotoxin against Triple Negative Breast Cancer (TNBC) -- Chapter 4. Conclusion and Discussion -- Bibliography. 330 $aThis thesis demonstrates a technology that enables pipetting-free high-throughput screening (HTS) on a miniaturized platform, eliminating the need for thousands of one-by-one pipetting and conventional liquid handling systems. This platform enhances accessibility to HTS and enables HTS to be used in small-to-medium scale laboratories. In addition, it allows large-scale combinatorial screening with a small number of valuable cells, such as patients? primary cancer cells. This technique will have a high impact for widespread use of HTS in the era of personalized medicine. In this thesis, the author firstly describes the need and concept of ?partipetting? for pipetting-free HTS platform. It is realized by the one-step pipetting and self-assembly of encoded drug-laden microparticles (DLPs) on the microwells. Next, the technical implementations required for the platform demonstration are described. It includes preparation of encoded DLPs, plastic chip fabrication, and realization of automated system. Lastly, screening of sequential drug combinations using this platform is demonstrated. This shows the potential of the proposed technology for various applications. 410 0$aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053 606 $aBiomedical engineering 606 $aMicroarrays 606 $aAmorphous substances 606 $aComplex fluids 606 $aNanotechnology 606 $aMicrobiology 606 $aBiotechnology 606 $aBiomedical Engineering and Bioengineering$3https://scigraph.springernature.com/ontologies/product-market-codes/T2700X 606 $aMicroarrays$3https://scigraph.springernature.com/ontologies/product-market-codes/B12050 606 $aSoft and Granular Matter, Complex Fluids and Microfluidics$3https://scigraph.springernature.com/ontologies/product-market-codes/P25021 606 $aNanotechnology and Microengineering$3https://scigraph.springernature.com/ontologies/product-market-codes/T18000 606 $aApplied Microbiology$3https://scigraph.springernature.com/ontologies/product-market-codes/C12010 606 $aMicroengineering$3https://scigraph.springernature.com/ontologies/product-market-codes/C12040 615 0$aBiomedical engineering. 615 0$aMicroarrays. 615 0$aAmorphous substances. 615 0$aComplex fluids. 615 0$aNanotechnology. 615 0$aMicrobiology. 615 0$aBiotechnology. 615 14$aBiomedical Engineering and Bioengineering. 615 24$aMicroarrays. 615 24$aSoft and Granular Matter, Complex Fluids and Microfluidics. 615 24$aNanotechnology and Microengineering. 615 24$aApplied Microbiology. 615 24$aMicroengineering. 676 $a610.28 700 $aSong$b Seo Woo$4aut$4http://id.loc.gov/vocabulary/relators/aut$0929406 906 $aBOOK 912 $a9910350294303321 996 $aOne-Step Generation of a Drug-Releasing Microarray for High-Throughput Small-Volume Bioassays$92088884 997 $aUNINA