LEADER 04243nam 22006615 450 001 9910349508303321 005 20200706230039.0 010 $a1-4939-9726-2 024 7 $a10.1007/978-1-4939-9726-8 035 $a(CKB)4100000009362510 035 $a(DE-He213)978-1-4939-9726-8 035 $a(MiAaPQ)EBC5899820 035 $a(PPN)269147152 035 $a(EXLCZ)994100000009362510 100 $a20190919d2019 u| 0 101 0 $aeng 135 $aurnn|008mamaa 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aBiophysics of RNA-Protein Interactions $eA Mechanistic View /$fedited by Chirlmin Joo, David Rueda 205 $a1st ed. 2019. 210 1$aNew York, NY :$cSpringer New York :$cImprint: Springer,$d2019. 215 $a1 online resource (VII, 249 p. 77 illus., 74 illus. in color.) 225 1 $aBiological and Medical Physics, Biomedical Engineering,$x1618-7210 311 $a1-4939-9724-6 320 $aIncludes bibliographical references. 327 $aPart 1: RNA Binding Proteins -- Chapter 1: How proteins recognize RNA -- Chapter 2: The interaction between L7Ae family of proteins and RNA kink turns -- Chapter 3: Evolving methods in defining the role of RNA in RNP assembly -- Chapter 4: Single-molecule studies of exonuclease: Following cleavage actions one step at a time -- Chapter 5: Fitting in the age of single-molecule experiments: A guide to maximum-likelihood estimation and its advantages -- Part II: Transcription and Translation -- Chapter 6: A single-molecule view on cellular and viral RNA synthesis -- Chapter 7: Single-Molecule Optical Tweezers Studies of Translation -- Part III: RNA-Guided Protein Machineries -- Chapter 8: Biophysical and biochemical approaches in the analysis of Argonaute-miRNA complexes -- Chapter 9: Biophysics of RNA-guided CRISPR immunity -- Chapter 10: Dynamics of MicroRNA Biogenesis. 330 $aRNA molecules play key roles in all aspects of cellular life, but to do so efficiently, they must work in synergism with proteins. This book addresses how proteins and RNA interact to carry out biological functions such as protein synthesis, regulation of gene expression, genome defense, liquid phase separation and more. The topics addressed in this volume will appeal to researchers in biophysics, biochemistry and structural biology. The book is a useful resource for anybody interested in elucidating the molecular mechanisms and discrete properties of RNA-protein complexes. Included are reviews of key systems such as microRNA and CRISPR/Cas that exemplify how RNA and proteins work together to perform their biological function. Also covered are techniques ranging from single molecule fluorescence and force spectroscopy to crystallography, cryo-EM microscopy, and kinetic modeling. 410 0$aBiological and Medical Physics, Biomedical Engineering,$x1618-7210 606 $aBiophysics 606 $aBiophysics 606 $aBiochemical engineering 606 $aProteins 606 $aAnalytical chemistry 606 $aBiological and Medical Physics, Biophysics$3https://scigraph.springernature.com/ontologies/product-market-codes/P27008 606 $aBiochemical Engineering$3https://scigraph.springernature.com/ontologies/product-market-codes/C12029 606 $aProtein Science$3https://scigraph.springernature.com/ontologies/product-market-codes/L14040 606 $aAnalytical Chemistry$3https://scigraph.springernature.com/ontologies/product-market-codes/C11006 615 0$aBiophysics. 615 0$aBiophysics. 615 0$aBiochemical engineering. 615 0$aProteins. 615 0$aAnalytical chemistry. 615 14$aBiological and Medical Physics, Biophysics. 615 24$aBiochemical Engineering. 615 24$aProtein Science. 615 24$aAnalytical Chemistry. 676 $a571.4 702 $aJoo$b Chirlmin$4edt$4http://id.loc.gov/vocabulary/relators/edt 702 $aRueda$b David$4edt$4http://id.loc.gov/vocabulary/relators/edt 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910349508303321 996 $aBiophysics of RNA-Protein Interactions$92506796 997 $aUNINA