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035   $a(DE-He213)978-3-030-29439-7
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100   $a20190926d2019      u|         0
101 0 $aeng
135   $aurnn|008mamaa
181   $ctxt$2rdacontent
182   $cc$2rdamedia
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200 10$aStructural and Biochemical Characterization of the YaxAB Pore-forming Toxin from Yersinia Enterocolitica /$fby Bastian Bräuning
205   $a1st ed. 2019.
210  1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2019.
215   $a1 online resource (XXXI, 86 p.) 
225 1 $aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053
311   $a3-030-29438-2 
327   $aIntroduction -- Objectives -- Materials & Methods -- Results -- Discussion.
330   $aThis book discusses the decoding of the lytic mechanism of an ?-helical pore-forming toxin, YaxAB, composed of two different subunits. Pore-forming toxins (PFTs) are among the most common bacterial toxins. They are produced by a variety of pathogens, which infect a wide range of organisms including plants, insects and humans. Yet the maturation of these particles and the structural changes required for pore formation are still poorly understood for many PFT families. Using a diverse panel of biochemical and structural techniques, including X-ray crystallography and cryo-electron microscopy, Dr. Bräuning and colleagues have succeeded in identifying the mechanistic contributions of the two toxin components and elucidating the lytic state of the pore complex. The results of this thesis on the YaxAB system are applicable to orthologues from agriculturally relevant insect pathogens, and offer valuable structural and mechanistic insights to inform future bioengineering efforts. .
410  0$aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053
606   $aMicrobiology
606   $aProteins 
606   $aAnalytical chemistry
606   $aBiochemical engineering
606   $aBacteriology
606   $aApplied Microbiology$3https://scigraph.springernature.com/ontologies/product-market-codes/C12010
606   $aProtein Science$3https://scigraph.springernature.com/ontologies/product-market-codes/L14040
606   $aAnalytical Chemistry$3https://scigraph.springernature.com/ontologies/product-market-codes/C11006
606   $aBiochemical Engineering$3https://scigraph.springernature.com/ontologies/product-market-codes/C12029
606   $aBacteriology$3https://scigraph.springernature.com/ontologies/product-market-codes/L23012
615  0$aMicrobiology.
615  0$aProteins .
615  0$aAnalytical chemistry.
615  0$aBiochemical engineering.
615  0$aBacteriology.
615 14$aApplied Microbiology.
615 24$aProtein Science.
615 24$aAnalytical Chemistry.
615 24$aBiochemical Engineering.
615 24$aBacteriology.
676   $a660.62
676   $a615.95
700   $aBräuning$b Bastian$4aut$4http://id.loc.gov/vocabulary/relators/aut$0780977
801  0$bMiAaPQ
801  1$bMiAaPQ
801  2$bMiAaPQ
906   $aBOOK
912   $a9910349507303321
996   $aStructural and Biochemical Characterization of the YaxAB Pore-forming Toxin from Yersinia Enterocolitica$91668134
997   $aUNINA