LEADER 04184nam 22005895 450 001 9910349503703321 005 20200706202221.0 010 $a3-030-31057-4 024 7 $a10.1007/978-3-030-31057-8 035 $a(CKB)4100000009678225 035 $a(MiAaPQ)EBC5968938 035 $a(DE-He213)978-3-030-31057-8 035 $a(PPN)258304723 035 $a(EXLCZ)994100000009678225 100 $a20191028d2019 u| 0 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$a15N Tracing of Microbial Assimilation, Partitioning and Transport of Fertilisers in Grassland Soils /$fby Alice Fiona Charteris 205 $a1st ed. 2019. 210 1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2019. 215 $a1 online resource (289 pages) $cillustrations 225 1 $aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053 311 $a3-030-31056-6 320 $aIncludes bibliographical references. 327 $aIntroduction -- Sites, sampling, materials and methods -- Compound-specific amino acid 15N stable isotope probing of nitrogen assimilation by the soil microbial biomass using gas chromatography-combustion- isotope ratio mass spectrometry -- Biosynthetic routing, rates and extents of microbial fertiliser nitrogen assimilation in two grazed grassland soils -- Microbial fertiliser nitrogen assimilation in the field as compared with the laboratory incubation experiments -- 15N tracing of the partitioning and transport of nitrate under field conditions -- Overview and future work. 330 $aThis book presents innovative research on soil nitrogen cycling and nitrate leaching with a view to improving soil management and fertiliser nitrogen use efficiency and reducing nitrogen leaching losses. In this regard, nitrogen-15 (15N)-labelled fertiliser was used as a biochemical and physical stable isotope tracer in laboratory and field experiments. The major outcome of the research was the development, validation and application of a new compound-specific amino acid 15N stable isotope probing method for assessing the assimilation of fertiliser nitrogen by soil microbial biomass. The novelty of the method lies in its tracing of incorporated nitrogen into newly biosynthesised microbial protein in time-course experiments using gas chromatography-combustion-isotope ratio mass spectrometry. The approach provides previously unattainable insights into the microbial processing of different nitrogen fertilisers in different soils. Further, it identifies the mechanistic link between molecular-scale processes and observations of field-scale fertiliser nitrogen immobilisation studies. The method and the results presented here will have far-reaching implications for the development of enhanced recommendations concerning farm-based soil management practices for increasing soil productivity and reducing nitrogen losses, which is essential to minimising environmental impacts. 410 0$aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053 606 $aAnalytical chemistry 606 $aEnvironmental chemistry 606 $aAgriculture 606 $aAnalytical Chemistry$3https://scigraph.springernature.com/ontologies/product-market-codes/C11006 606 $aEnvironmental Chemistry$3https://scigraph.springernature.com/ontologies/product-market-codes/U15000 606 $aAgriculture$3https://scigraph.springernature.com/ontologies/product-market-codes/L11006 615 0$aAnalytical chemistry. 615 0$aEnvironmental chemistry. 615 0$aAgriculture. 615 14$aAnalytical Chemistry. 615 24$aEnvironmental Chemistry. 615 24$aAgriculture. 676 $a577.145 676 $a574.52636 700 $aCharteris$b Alice Fiona$4aut$4http://id.loc.gov/vocabulary/relators/aut$01063689 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910349503703321 996 $a15N Tracing of Microbial Assimilation, Partitioning and Transport of Fertilisers in Grassland Soils$92533678 997 $aUNINA