LEADER 03263nam 2200433z- 450 001 9910346752703321 005 20231214132835.0 035 $a(CKB)4920000000094187 035 $a(oapen)https://directory.doabooks.org/handle/20.500.12854/48269 035 $a(EXLCZ)994920000000094187 100 $a20202102d2018 |y 0 101 0 $aeng 135 $aurmn|---annan 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aGame Changer - Next Generation Sequencing and its Impact on Food Microbiology 210 $cFrontiers Media SA$d2018 215 $a1 electronic resource (302 p.) 225 1 $aFrontiers Research Topics 311 $a2-88945-463-0 330 $aAdvances in next-generation sequencing technologies (NGS) are revolutionizing the field of food microbiology. Microbial whole genome sequencing (WGS) can provide identification, characterization, and subtyping of pathogens for epidemiological investigations at a level of precision previously not possible. This allows for connections and source attribution to be inferred between related isolates that may be overlooked by traditional techniques. The archiving and global sharing of genome sequences allow for retrospective analysis of virulence genes, antimicrobial resistance markers, mobile genetic elements and other novel genes. The advent of high-throughput 16S rRNA amplicon sequencing, in combination with the advantages offered by massively parallel second-generation sequencing for metagenomics, enable intensive studies on the microbiomes of food products and the impact of foods on the human microbiome. These studies may one day lead to the development of reliable culture-independent methods for food monitoring and surveillance. Similarly, RNA-seq has provided insights into the transcriptomes and hence the behaviour of bacterial pathogens in food, food processing environments, and in interaction with the host at a resolution previously not achieved through the use of microarrays and/or RT-PCR. The vast un-tapped potential applications of NGS along with its rapidly declining costs, give this technology the ability to contribute significantly to consumer protection, global trade facilitation, and increased food safety and security. Despite the rapid advances, challenges remain. How will NGS data be incorporated into our existing global food safety infrastructure? How will massive NGS data be stored and shared globally? What bioinformatics solutions will be used to analyse and optimise these large data sets? This Research Topic discusses recent advances in the field of food microbiology made possible through the use of NGS. 610 $aSalmonella 610 $aNorovirus 610 $aFoodborne 610 $aListeria 610 $aFood Safety 610 $aFood Microbiology 610 $aNext Generation Sequencing 610 $aWhole Genome Sequencing 610 $aMicrobiome 610 $aVibrio 700 $aSandra Torriani$4auth$01317905 702 $aSabah Bidawid$4auth 702 $aJennifer Ronholm$4auth 906 $aBOOK 912 $a9910346752703321 996 $aGame Changer - Next Generation Sequencing and its Impact on Food Microbiology$93033075 997 $aUNINA