LEADER 04922nam 22005535 450 001 9910337955503321 005 20231130232848.0 010 $a981-13-6354-4 024 7 $a10.1007/978-981-13-6355-9 035 $a(CKB)5070000000000446 035 $a(DE-He213)978-981-13-6355-9 035 $a(MiAaPQ)EBC5925111 035 $a(PPN)235229911 035 $a(EXLCZ)995070000000000446 100 $a20190325d2019 u| 0 101 0 $aeng 135 $aurnn#008mamaa 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 12$aA Practical Guide to Pharmacological Biotechnology /$fby Jayanta Kumar Patra, Swagat Kumar Das, Gitishree Das, Hrudayanath Thatoi 205 $a1st ed. 2019. 210 1$aSingapore :$cSpringer Singapore :$cImprint: Springer,$d2019. 215 $a1 online resource (XIII, 142 p. 50 illus., 39 illus. in color.) 225 1 $aLearning Materials in Biosciences,$x2509-6125 311 $a981-13-6355-2 327 $aPart1.Introduction -- 1.1 Rules of laboratory safety in a pharmacology laboratory -- 1.2 Common instruments and their uses used in a pharmacology laboratory -- Part2.General aspects of Pharmacology laboratory -- 2.1 Safety and Risk assessment -- 2.2 Use and Handling of laboratory animals -- 2.3 Experimental design -- 2.4 Essential statistics -- 2.5 Cumulative Dose Response Curve -- 2.6 Toxicology -- 2.7 Basic instruments and techniques in Pharmacology Laboratory -- Part3.Isolated tissues and organs -- 3.1 Basic instruments used for isolated tissue experiments -- 3.2 Organ baths -- 3.3 Smooth muscle preparations -- 3.4 Skeletal muscle preparations -- 3.5 Cardiac muscle preparations -- Part4.Isolated tissues for screening of drugs -- 4.1 Evaluation of antidiabetic agents -- 4.2 Evaluation of antidepressants -- 4.3 Evaluation of antihypertensive agents -- 4.4 Evaluation of antiulcer agents -- 4.5 Evaluation of hepatotoxicity -- 4.6 Evaluation of Antioxidant agents -- 4.7 Evaluation of local anaesthetics -- Part5.Genotoxicity and Toxicological studies -- 5.1 The Mouse Lymphoma Assay -- 5.2 The Comet Assay -- 5.3 In vitro Genotoxicity assay -- 5.4 In vitro Teratogenicity Testing -- 5.5 Histopathological studies of animal tissues -- 5.6 Drug poisoning -- Part6.Experimental Animal studies -- 6.1 Pyrogen testing -- 6.2 Collecting blood from mice -- 6.3 Studies on different parameters of blood -- 6.4 Experiment on central nervous system -- 6.5 Evaluation on cardiovascular system -- 6.6 Experiments on GI tract -- Part7.Clinical trials -- 7.1 Clinical Pharmacology and its genesis -- 7.2 National and International agencies and their role in Clinical pharmacology -- 7.3 Stages in drug development and clinical trials -- 7.4 Ethics in Clinical research -- 7.5 Safety assessment in Clinical trials -- Part8.IPR and ethics in animal studies -- 8.1 Intellectual property rights and its different categories -- 8.2 Importance of IPR in drug development -- 8.3 Patenting cells, cell lines and animals -- 8.4 Ethics in laboratory animal studies -- 8.5 Risk assessment and management -- 8.6 Good laboratory practices. 330 $aPharmacological biotechnology is applied to and used to study drug development, working mechanisms, diagnosis, and therapies. This manual is the textbook covering the whole range of experiments related to pharmacology. It also contains basic laboratory safety guidelines along with the basic calculations and formulas used in a laboratory. Each chapter starts with an introduction/theory into the basic approach followed by detailed methods sections with easy-to-follow protocols and comprehensive troubleshooting, calculations and possible questions for examination. The target group is researchers who are studying pharmacological biotechnology in the laboratory. 410 0$aLearning Materials in Biosciences,$x2509-6125 606 $aLaboratory medicine 606 $aPharmaceutical technology 606 $aLaboratory Medicine$3https://scigraph.springernature.com/ontologies/product-market-codes/B15007 606 $aPharmaceutical Sciences/Technology$3https://scigraph.springernature.com/ontologies/product-market-codes/B21010 615 0$aLaboratory medicine. 615 0$aPharmaceutical technology. 615 14$aLaboratory Medicine. 615 24$aPharmaceutical Sciences/Technology. 676 $a610.284 700 $aPatra$b Jayanta Kumar$4aut$4http://id.loc.gov/vocabulary/relators/aut$0849596 702 $aDas$b Swagat Kumar$4aut$4http://id.loc.gov/vocabulary/relators/aut 702 $aDas$b Gitishree$4aut$4http://id.loc.gov/vocabulary/relators/aut 702 $aThatoi$b Hrudayanath$4aut$4http://id.loc.gov/vocabulary/relators/aut 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910337955503321 996 $aA Practical Guide to Pharmacological Biotechnology$92225261 997 $aUNINA