LEADER 05030nam 22007455 450 001 9910300538303321 005 20200701105006.0 010 $a4-431-56618-X 024 7 $a10.1007/978-4-431-56618-2 035 $a(CKB)4100000002892572 035 $a(DE-He213)978-4-431-56618-2 035 $a(MiAaPQ)EBC6303620 035 $a(MiAaPQ)EBC5578899 035 $a(Au-PeEL)EBL5578899 035 $a(OCoLC)1030411764 035 $a(PPN)22554928X 035 $a(EXLCZ)994100000002892572 100 $a20180329d2018 u| 0 101 0 $aeng 135 $aurnn#008mamaa 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aX-Ray Diffraction Imaging of Biological Cells /$fby Masayoshi Nakasako 205 $a1st ed. 2018. 210 1$aTokyo :$cSpringer Japan :$cImprint: Springer,$d2018. 215 $a1 online resource (XX, 228 p. 96 illus., 89 illus. in color.) 225 1 $aSpringer Series in Optical Sciences,$x0342-4111 ;$v210 311 $a4-431-56616-3 327 $aIntroduction -- X-ray diffraction -- Theory of X-ray diffraction imaging -- Diffraction apparatus for X-ray diffraction imaging -- Specimen preparation for X-ray diffraction imaging experiments at cryogenic temperature -- Processing of diffraction patterns obtained from X-ray diffraction imaging experiments using X-ray free electron laser pulses -- Phase retrieval of diffraction patterns -- Projection structures of biological cells and organelles -- Three-dimensional structural analyses in cryogenic X-ray diffraction imaging -- Prospects for the structural analysis of biological specimens by X-ray diffraction imaging. 330 $aIn this book, the author describes the development of the experimental diffraction setup and structural analysis of non-crystalline particles from material science and biology. Recent advances in X-ray free electron laser (XFEL)-coherent X-ray diffraction imaging (CXDI) experiments allow for the structural analysis of non-crystalline particles to a resolution of 7 nm, and to a resolution of 20 nm for biological materials. Now XFEL-CXDI marks the dawn of a new era in structural analys of non-crystalline particles with dimensions larger than 100 nm, which was quite impossible in the 20th century. To conduct CXDI experiments in both synchrotron and XFEL facilities, the author has developed apparatuses, named KOTOBUKI-1 and TAKASAGO-6 for cryogenic diffraction experiments on frozen-hydrated non-crystalline particles at around 66 K. At the synchrotron facility, cryogenic diffraction experiments dramatically reduce radiation damage of specimen particles and allow tomography CXDI experiments. In addition, in XFEL experiments, non-crystalline particles scattered on thin support membranes and flash-cooled can be used to efficiently increase the rate of XFEL pulses. The rate, which depends on the number density of scattered particles and the size of X-ray beams, is currently 20-90%, probably the world record in XFEL-CXDI experiments. The experiment setups and results are introduced in this book. The author has also developed software suitable for efficiently processing of diffraction patterns and retrieving electron density maps of specimen particles based on the diffraction theory used in CXDI. 410 0$aSpringer Series in Optical Sciences,$x0342-4111 ;$v210 606 $aLasers 606 $aPhotonics 606 $aMaterials science 606 $aProteins  606 $aCrystallography 606 $aPhysical measurements 606 $aMeasurement    606 $aOptics, Lasers, Photonics, Optical Devices$3https://scigraph.springernature.com/ontologies/product-market-codes/P31030 606 $aCharacterization and Evaluation of Materials$3https://scigraph.springernature.com/ontologies/product-market-codes/Z17000 606 $aProtein Structure$3https://scigraph.springernature.com/ontologies/product-market-codes/L14050 606 $aCrystallography and Scattering Methods$3https://scigraph.springernature.com/ontologies/product-market-codes/P25056 606 $aMeasurement Science and Instrumentation$3https://scigraph.springernature.com/ontologies/product-market-codes/P31040 615 0$aLasers. 615 0$aPhotonics. 615 0$aMaterials science. 615 0$aProteins . 615 0$aCrystallography. 615 0$aPhysical measurements. 615 0$aMeasurement   . 615 14$aOptics, Lasers, Photonics, Optical Devices. 615 24$aCharacterization and Evaluation of Materials. 615 24$aProtein Structure. 615 24$aCrystallography and Scattering Methods. 615 24$aMeasurement Science and Instrumentation. 676 $a548.83 700 $aNakasako$b Masayoshi$4aut$4http://id.loc.gov/vocabulary/relators/aut$0835600 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910300538303321 996 $aX-Ray Diffraction Imaging of Biological Cells$91867730 997 $aUNINA