LEADER 05490nam 22006975 450 001 9910300528503321 005 20200707012840.0 010 $a3-319-73148-3 024 7 $a10.1007/978-3-319-73148-3 035 $a(CKB)4100000004832016 035 $a(DE-He213)978-3-319-73148-3 035 $a(MiAaPQ)EBC5419766 035 $a(PPN)229494935 035 $a(EXLCZ)994100000004832016 100 $a20180608d2018 u| 0 101 0 $aeng 135 $aurnn|008mamaa 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aMembrane Proteins in Aqueous Solutions$b[electronic resource] $eFrom Detergents to Amphipols /$fby Jean-Luc Popot 205 $a1st ed. 2018. 210 1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2018. 215 $a1 online resource (XXVII, 708 p. 437 illus., 266 illus. in color.) 225 1 $aBiological and Medical Physics, Biomedical Engineering,$x1618-7210 311 $a3-319-73146-7 327 $aFrom the Contents: Membrane proteins: functions, structures, environments -- Taking membrane proteins out of their natural environment -- Alternatives to detergents for handling membrane proteins in aqueous solutions -- Chemical structure and physical-chemical properties of amphipols. 330 $aMembrane proteins represent about one third of the proteins encoded in a cell's genome, and, because of their key physiological roles, more than half of drug targets. Detergents are traditionally used to extract proteins from membranes in order to make them amenable to the tools of biochemistry and biophysics. However, detergent-solubilized proteins are generally unstable. This has led to the development of alternative, non-conventional surfactants, such as bicelles, nanodiscs, amphipathic peptides, fluorinated surfactants, and specially designed amphipathic polymers called 'amphipols'. These novel tools, mainly developed over the past 20 years, are revolutionizing handling membrane proteins in vitro for basic and applied research, as well as for such biomedical applications as drug screening or vaccination. This book, written by a specialist of membrane proteins and one of the creators of amphipols, describes the properties and uses of these novel molecules. It opens with general introductions on membrane proteins and their natural environment, detergents, the current status of membrane protein in vitro studies, a broad panorama of non-conventional surfactants and a discussion of their respective advantages and limitations, and the preparation and properties of amphipols and membrane protein/amphipol complexes. Topical chapters cover in vitro folding, cell-free synthesis and stabilization of membrane proteins, and such biophysical and biochemical applications as electron microscopy, Xray diffraction, NMR, optical spectroscopy, mass spectrometry, the whole range of solutions studies, proteomics, and such practical applications as membrane protein immobilization and drug screening and the use of amphipols in vivo for vaccination and drug delivery. Each topical chapter is introduced with a concise, up-to-date overview of how membrane proteins are currently studied using each individual technique, before offering an exhaustive coverage and in-depth discussion of the contribution of amphipols, and concluding with hands-on protocols written by everyday practitioners of each application. In addition to a comprehensive coverage of the properties and uses of non-conventional surfactants, this book therefore also offers a concise, accessible introduction to membrane protein biochemistry and biophysics. It is meant to be used both in basic and applied research laboratories and as a teaching help. 410 0$aBiological and Medical Physics, Biomedical Engineering,$x1618-7210 606 $aBiophysics 606 $aBiological physics 606 $aMedicinal chemistry 606 $aProteins  606 $aPharmaceutical technology 606 $aPolymers   606 $aPhysical chemistry 606 $aBiological and Medical Physics, Biophysics$3https://scigraph.springernature.com/ontologies/product-market-codes/P27008 606 $aMedicinal Chemistry$3https://scigraph.springernature.com/ontologies/product-market-codes/C28000 606 $aProtein Science$3https://scigraph.springernature.com/ontologies/product-market-codes/L14040 606 $aPharmaceutical Sciences/Technology$3https://scigraph.springernature.com/ontologies/product-market-codes/B21010 606 $aPolymer Sciences$3https://scigraph.springernature.com/ontologies/product-market-codes/C22008 606 $aPhysical Chemistry$3https://scigraph.springernature.com/ontologies/product-market-codes/C21001 615 0$aBiophysics. 615 0$aBiological physics. 615 0$aMedicinal chemistry. 615 0$aProteins . 615 0$aPharmaceutical technology. 615 0$aPolymers  . 615 0$aPhysical chemistry. 615 14$aBiological and Medical Physics, Biophysics. 615 24$aMedicinal Chemistry. 615 24$aProtein Science. 615 24$aPharmaceutical Sciences/Technology. 615 24$aPolymer Sciences. 615 24$aPhysical Chemistry. 676 $a571.4 700 $aPopot$b Jean-Luc$4aut$4http://id.loc.gov/vocabulary/relators/aut$01060606 906 $aBOOK 912 $a9910300528503321 996 $aMembrane Proteins in Aqueous Solutions$92514566 997 $aUNINA