LEADER 05218nam 22008055 450 001 9910300370703321 005 20200701045817.0 010 $a94-007-7545-8 024 7 $a10.1007/978-94-007-7545-9 035 $a(CKB)3710000000078942 035 $a(DE-He213)978-94-007-7545-9 035 $a(SSID)ssj0001090808 035 $a(PQKBManifestationID)11620084 035 $a(PQKBTitleCode)TC0001090808 035 $a(PQKBWorkID)11021469 035 $a(PQKB)11492558 035 $a(MiAaPQ)EBC6314171 035 $a(MiAaPQ)EBC1591825 035 $a(Au-PeEL)EBL1591825 035 $a(CaPaEBR)ebr10968921 035 $a(OCoLC)922907332 035 $z(PPN)20051511X 035 $a(PPN)176129561 035 $a(EXLCZ)993710000000078942 100 $a20131212d2014 u| 0 101 0 $aeng 135 $aurnn|008mamaa 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aFundamentals of Fluorescence Microscopy $eExploring Life with Light /$fby Partha Pratim Mondal, Alberto Diaspro 205 $a1st ed. 2014. 210 1$aDordrecht :$cSpringer Netherlands :$cImprint: Springer,$d2014. 215 $a1 online resource (XV, 218 p. 140 illus., 55 illus. in color.) 300 $aBibliographic Level Mode of Issuance: Monograph 311 $a94-007-7544-X 327 $aPreface -- Acknowledgements -- Dedication -- Ray Optics, Wave Optics and Imaging System Designing -- Basics of Electromagnetic Theory for Fluorescence Microscopy -- Electric Field Effects in Optical Microscopy Systems -- Quantum Description of Radiation Field and Optical Microscopy -- Molecular Physics of Fluorescence Markers -- Basics of Fluorescence and Photophysics -- General Fluorescence Imaging Techniques -- Multiphoton Fluorescence Microscopy -- Super Resolution Fluorescence Microscopy -- Image Reconstruction Methodologies for Fluorescence Microscopy -- Future Prospective of Fluorescence Microscopy -- Appendix I -- Appendix II -- Appendix III. 330 $aThis book starts at an introductory level and leads reader to the most advanced developments in fluorescence imaging and super-resolution techniques that have enabled the emergence of new disciplines such as nanobioimaging, multiphoton microscopy, photodynamic therapy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going deep into the advanced imaging systems and their applications. Molecular orbital theory forms the basis for understanding fluorescent molecules and thereby facilitates complete explanation of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential fluorescence factors and phenomena such as, molecular cross-section, Stokes shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These phenomena form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part covers many imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction limited and unlimited imaging. 606 $aSpectrum analysis 606 $aMicroscopy 606 $aBiophysics 606 $aBiophysics 606 $aLasers 606 $aPhotonics 606 $aSpectroscopy and Microscopy$3https://scigraph.springernature.com/ontologies/product-market-codes/P31090 606 $aBiological Microscopy$3https://scigraph.springernature.com/ontologies/product-market-codes/L26000 606 $aBiological and Medical Physics, Biophysics$3https://scigraph.springernature.com/ontologies/product-market-codes/P27008 606 $aOptics, Lasers, Photonics, Optical Devices$3https://scigraph.springernature.com/ontologies/product-market-codes/P31030 606 $aSpectroscopy/Spectrometry$3https://scigraph.springernature.com/ontologies/product-market-codes/C11020 615 0$aSpectrum analysis. 615 0$aMicroscopy. 615 0$aBiophysics. 615 0$aBiophysics. 615 0$aLasers. 615 0$aPhotonics. 615 14$aSpectroscopy and Microscopy. 615 24$aBiological Microscopy. 615 24$aBiological and Medical Physics, Biophysics. 615 24$aOptics, Lasers, Photonics, Optical Devices. 615 24$aSpectroscopy/Spectrometry. 676 $a502.82 700 $aMondal$b Partha Pratim$4aut$4http://id.loc.gov/vocabulary/relators/aut$01064213 702 $aDiaspro$b Alberto$4aut$4http://id.loc.gov/vocabulary/relators/aut 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910300370703321 996 $aFundamentals of Fluorescence Microscopy$92536896 997 $aUNINA