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101 0 $aeng
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181   $ctxt$2rdacontent
182   $cc$2rdamedia
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200 10$aStudy of the Calcium Regulation Mechanism of TCR Activation Using Nanodisc and NMR Technologies /$fby Yunchen Bi
205   $a1st ed. 2018.
210  1$aBerlin, Heidelberg :$cSpringer Berlin Heidelberg :$cImprint: Springer,$d2018.
215   $a1 online resource (XII, 79 p. 41 illus.) 
225 1 $aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053
311 08$a3-662-54616-7 
327   $aIntroduction -- Review -- Nanodisc assembly and the study on the interaction between the lipid and ion -- Calcium regulates T cell receptor activation through phospholipid electrostatic manipulation -- The preliminary study on the structure of Dengue Virus NS2B-NS3p in complex with aprotinin -- Summary and prospect of this dissertation.
330   $aThis thesis describes the use of biophysical and biochemical methods to prove that calcium has a positive feedback effect on amplifying and sustaining CD3 phosphorylation and should enhance T-cell sensitivity to foreign antigens. The study presented shows that calcium can regulate the signal pathway in cells not only as a secondary messenger but also through direct interactions with the phospholipid bilayer. The approach used in the thesis also represents an important advance, as it couples the use of nuclear magnetic resonance (NMR) to the analysis of signaling phenomena in living cells. Moreover, the thesis optimizes the Nanodisc assembly protocol, which can broaden its range of applications in membrane protein studies. A preliminary study on the structure of dengue virus NS2B-NS3p in complex with aprotinin, which may help to develop new drugs against the dengue virus, is also included.
410  0$aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053
606   $aBiomedical engineering
606   $aBiophysics
606   $aBiophysics
606   $aCell membranes
606   $aImmunology
606   $aAtomic structure
606   $aMolecular structure
606   $aBiomedical Engineering and Bioengineering$3https://scigraph.springernature.com/ontologies/product-market-codes/T2700X
606   $aBiological and Medical Physics, Biophysics$3https://scigraph.springernature.com/ontologies/product-market-codes/P27008
606   $aMembrane Biology$3https://scigraph.springernature.com/ontologies/product-market-codes/L16050
606   $aImmunology$3https://scigraph.springernature.com/ontologies/product-market-codes/B14000
606   $aAtomic/Molecular Structure and Spectra$3https://scigraph.springernature.com/ontologies/product-market-codes/P24017
615  0$aBiomedical engineering.
615  0$aBiophysics.
615  0$aBiophysics.
615  0$aCell membranes.
615  0$aImmunology.
615  0$aAtomic structure.
615  0$aMolecular structure.
615 14$aBiomedical Engineering and Bioengineering.
615 24$aBiological and Medical Physics, Biophysics.
615 24$aMembrane Biology.
615 24$aImmunology.
615 24$aAtomic/Molecular Structure and Spectra.
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906   $aBOOK
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996   $aStudy of the Calcium Regulation Mechanism of TCR Activation Using Nanodisc and NMR Technologies$92495614
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