LEADER 02692oam 2200481 450 001 9910298640403321 005 20190911112725.0 010 $a3-319-02159-1 024 7 $a10.1007/978-3-319-02159-1 035 $a(OCoLC)863379270 035 $a(MiFhGG)GVRL6XGY 035 $a(EXLCZ)993710000000057964 100 $a20130829d2014 uy 0 101 0 $aeng 135 $aurun|---uuuua 181 $ctxt 182 $cc 183 $acr 200 10$aBiomolecular imaging at high spatial and temporal resolution in vitro and in vivo /$fThomas Harry Sharp 205 $a1st ed. 2014. 210 1$aCham [Switzerland] :$cSpringer,$d2014. 215 $a1 online resource (xviii, 150 pages) $cillustrations (some color) 225 1 $aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053 300 $a"ISSN: 2190-5053." 311 $a3-319-02158-3 320 $aIncludes bibliographical references. 327 $aBiomolecules & Microscopy -- Materials and Methods -- Experimental Methods -- Imaging Methods -- Experimental Results -- Towards an Atomistic Structure of a De Novo Designed Peptide Fibre -- Developing Novel Probes for Correlative Light Electron Microscopy. 330 $aAs part of a collaboration between two different groups in chemistry and biochemistry, Thom Sharp presents here his thesis work on the development of new methods for cryoelectron microscopy. Throughout his PhD, Thom had to master a whole range of techniques including modelling, molecular biology and microscopy. Using these skills to tackle an outstanding problem, the pursuit of high-resolution structures of peptide-based materials, Thom highlights in this thesis his newly developed methods for analysing and processing this particular type of electron microscopy data. This thesis gives the first molecular description of a de-novo designed peptide-based material. In general, this research will have a huge impact on the peptide assembly field, and also in electron microscopy as it introduces new methods and approaches, all of which are Thom's inventions and are described in this thesis. 410 0$aSpringer theses. 606 $aElectron microscopy 606 $aCryobiochemistry 606 $aPeptides$xStructure 615 0$aElectron microscopy. 615 0$aCryobiochemistry. 615 0$aPeptides$xStructure. 676 $a572.36 700 $aSharp$b Thomas Harry$4aut$4http://id.loc.gov/vocabulary/relators/aut$01065002 801 0$bMiFhGG 801 1$bMiFhGG 906 $aBOOK 912 $a9910298640403321 996 $aBiomolecular Imaging at High Spatial and Temporal Resolution In Vitro and In Vivo$92542213 997 $aUNINA