LEADER 02988oam  2200457   450 
001 9910298640103321
005 20190911112726.0
010   $a3-642-42008-7
024 7 $a10.1007/978-3-642-42008-5
035   $a(OCoLC)878127870
035   $a(MiFhGG)GVRL6WUK
035   $a(EXLCZ)993710000000074833
100   $a20131023d2014      uy             0
101 0 $aeng
135   $aurun|---uuuua
181   $ctxt
182   $cc
183   $acr
200 10$aApplications of monolithic column and isotope dimethylation labeling in shotgun proteome analysis /$fFangjun Wang
205   $a1st ed. 2014.
210  1$aHeidelberg, Germany :$cSpringer,$d2014.
215   $a1 online resource (xiii, 94 pages) $cillustrations (some color)
225 1 $aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053
300   $a"ISSN: 2190-5053."
311   $a3-642-42007-9 
320   $aIncludes bibliographical references.
327   $aIntroduction -- Online sample injection and multidimensional chromatography separation by using strong cation exchange monolithic column -- Development of polymer-based hydrophobic monolithic columns and their applications in proteome analysis -- Large-scale proteome and phosphoproteome quantification by using dimethylation isotope labeling.
330   $aIn this thesis, the author outlines the development of new monolithic columns and isotope dimethyl labeling strategies and their applications in high-performance proteome analyses. Though different types of monolithic columns have been widely developed for chromatography and electrophoresis separation, their application in proteomics for complex peptide mixtures separation is still a challenge. The author discusses the preparation of new monolithic columns and optimization of chromatography separation capability to improve coverage and accuracy of proteome analysis. Further, the author describes a novel online multidimensional chromatography system combined with automated online isotope labeling, which significantly improves the throughput, sensitivity, and accuracy of quantitative proteomics. In addition to the development of new technologies, the author investigates the proteome and phosphoproteome expression changes of clinical hepatocellular carcinoma tissues and the hippocampi of mice with Alzheimer?s disease. The work in this thesis has led to several publications in high-profile journals in the fields of analytical chemistry and proteome research.
410  0$aSpringer theses.
606   $aProteins$xAnalysis
606   $aProteomics
615  0$aProteins$xAnalysis.
615  0$aProteomics.
676   $a543.65
700   $aWang$b Fangjun$4aut$4http://id.loc.gov/vocabulary/relators/aut$01061947
801  0$bMiFhGG
801  1$bMiFhGG
906   $aBOOK
912   $a9910298640103321
996   $aApplications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis$92521559
997   $aUNINA