LEADER 04449nam 22007335 450 001 9910298633903321 005 20200702123012.0 010 $a3-662-47293-7 024 7 $a10.1007/978-3-662-47293-4 035 $a(CKB)3710000000416750 035 $a(EBL)2095480 035 $a(SSID)ssj0001500940 035 $a(PQKBManifestationID)11852693 035 $a(PQKBTitleCode)TC0001500940 035 $a(PQKBWorkID)11536264 035 $a(PQKB)10000603 035 $a(DE-He213)978-3-662-47293-4 035 $a(MiAaPQ)EBC2095480 035 $a(PPN)186027656 035 $a(EXLCZ)993710000000416750 100 $a20150526d2015 u| 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aMicroRNA Detection and Pathological Functions /$fby Xueji Zhang, Haifeng Dong, Yaping Tian 205 $a1st ed. 2015. 210 1$aBerlin, Heidelberg :$cSpringer Berlin Heidelberg :$cImprint: Springer,$d2015. 215 $a1 online resource (108 p.) 225 1 $aSpringerBriefs in Molecular Science,$x2191-5407 300 $aDescription based upon print version of record. 311 $a3-662-47292-9 320 $aIncludes bibliographical references at the end of each chapters. 327 $aIntroduction -- miRNAs Biology in Pathological Processes -- Conventional MiRNA Detection Strategies -- MiRNA Electrochemical Detection -- MiRNA Optical Detection.- Other Emerging MiRNA Detection Strategies -- Intracellular and Organic miRNA in Situ Detection -- Summary and Prospects. 330 $aThis book summarizes microRNA (miRNA) biology in a variety of pathological processes, emphasizing the significant potential applications of miRNA in diagnostics and prognostics, as well as novel drug targets. The conventional techniques used for miRNA detection including standard PCR, Northern blotting, microarray and clone methods are addressed. Recent emerging strategies in miRNA detection and quantification with superior flexibility and adaptability, such as novel molecular biological techniques and locked nucleic acid (LNA) modified probes, as well as nanotechnology-based approaches, are also included. The book also highlights the latest advances in clinical-related miRNA detection methods in living cells, circulating blood and tissue, such as in situ hybridization (ISH) and molecular imaging techniques, which are useful to elucidate the biogenesis and biological function of miRNAs in vivo. Finally, the respective advantages and drawbacks of various detection techniques in this fast-moving field are discussed, along with the challenges and promising new directions. This book offers a valuable resource for analytical chemists, biologists and physicians involved in miRNA research. Dr. Xueji Zhang and Dr. Haifeng Dong are Professors at the School of Chemistry & Biological Engineering, University of Science & Technology Beijing (USTB), China. Dr. Yaping Tian is a Professor at the Department of Clinical Biochemistry, Chinese PLA General Hospital and Military Medical School, China. 410 0$aSpringerBriefs in Molecular Science,$x2191-5407 606 $aAnalytical chemistry 606 $aCancer research 606 $aNucleic acids 606 $aGene therapy 606 $aAnalytical Chemistry$3https://scigraph.springernature.com/ontologies/product-market-codes/C11006 606 $aCancer Research$3https://scigraph.springernature.com/ontologies/product-market-codes/B11001 606 $aNucleic Acid Chemistry$3https://scigraph.springernature.com/ontologies/product-market-codes/L14011 606 $aGene Therapy$3https://scigraph.springernature.com/ontologies/product-market-codes/B12020 615 0$aAnalytical chemistry. 615 0$aCancer research. 615 0$aNucleic acids. 615 0$aGene therapy. 615 14$aAnalytical Chemistry. 615 24$aCancer Research. 615 24$aNucleic Acid Chemistry. 615 24$aGene Therapy. 676 $a572.88 700 $aZhang$b Xueji$4aut$4http://id.loc.gov/vocabulary/relators/aut$0991428 702 $aDong$b Haifeng$4aut$4http://id.loc.gov/vocabulary/relators/aut 702 $aTian$b Yaping$4aut$4http://id.loc.gov/vocabulary/relators/aut 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910298633903321 996 $aMicroRNA Detection and Pathological Functions$92268945 997 $aUNINA