LEADER 05191nam 22007335 450 001 9910298628903321 005 20200704115059.0 010 $a3-319-15636-5 024 7 $a10.1007/978-3-319-15636-1 035 $a(CKB)3710000000402861 035 $a(EBL)2096943 035 $a(SSID)ssj0001500911 035 $a(PQKBManifestationID)11918645 035 $a(PQKBTitleCode)TC0001500911 035 $a(PQKBWorkID)11522317 035 $a(PQKB)11218747 035 $a(DE-He213)978-3-319-15636-1 035 $a(MiAaPQ)EBC2096943 035 $a(PPN)185490247 035 $a(EXLCZ)993710000000402861 100 $a20150423d2015 u| 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aAdvanced Photon Counting$b[electronic resource] $eApplications, Methods, Instrumentation /$fedited by Peter Kapusta, Michael Wahl, Rainer Erdmann 205 $a1st ed. 2015. 210 1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2015. 215 $a1 online resource (371 p.) 225 1 $aSpringer Series on Fluorescence, Methods and Applications,$x1617-1306 ;$v15 300 $aDescription based upon print version of record. 311 $a3-319-15635-7 320 $aIncludes bibliographical references and index. 327 $aModern TCSPC electronics - Principles and Acquisition Modes -- Single-photon counting detectors for the visible range between 300 nm and 1000 nm -- Single-photon detectors for infrared wavelengths in the range 1 to 1.7 ?m -- Modern pulsed diode laser sources for time-correlated photon counting -- Advanced FCS: an Introduction to Fluorescence Lifetime Correlation Spectroscopy and Dual Focus FCS -- Lifetime-weighted FCS and 2D FLCS: Advanced application of time-tagged TCSPC -- MFD-PIE and PIE-FI: Ways to extract more information with TCSPC -- Photon Antibunching in Single Molecule Fluorescence Spectroscopy -- FLIM Strategies for Intracellular Sensing: Fluorescence Lifetime Imaging as a Tool to Quantify Analytes of Interest -- Multiple-Pulse Pumping with Time-Gated Detection for Enhanced Fluorescence Imaging in Cells and Tissue -- Pattern based linear un-mixing for efficient and reliable analysis of multi-component TCSPC-data -- Metal-Induced Energy Transfer -- The importance of photon arrival times in STED microscopy -- Single color centers in diamond as single photon sources and quantum sensors -- Photon counting and timing in quantum optics experiments -- Photon counting in diffuse optical imaging. 330 $aThis volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a ?fingerprint? for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight. 410 0$aSpringer Series on Fluorescence, Methods and Applications,$x1617-1306 ;$v15 606 $aSpectroscopy 606 $aLasers 606 $aPhotonics 606 $aBiophysics 606 $aBiological physics 606 $aSpectroscopy/Spectrometry$3https://scigraph.springernature.com/ontologies/product-market-codes/C11020 606 $aOptics, Lasers, Photonics, Optical Devices$3https://scigraph.springernature.com/ontologies/product-market-codes/P31030 606 $aBiological and Medical Physics, Biophysics$3https://scigraph.springernature.com/ontologies/product-market-codes/P27008 615 0$aSpectroscopy. 615 0$aLasers. 615 0$aPhotonics. 615 0$aBiophysics. 615 0$aBiological physics. 615 14$aSpectroscopy/Spectrometry. 615 24$aOptics, Lasers, Photonics, Optical Devices. 615 24$aBiological and Medical Physics, Biophysics. 676 $a54 676 $a543.2-543.8 676 $a571.4 676 $a621.36 702 $aKapusta$b Peter$4edt$4http://id.loc.gov/vocabulary/relators/edt 702 $aWahl$b Michael$4edt$4http://id.loc.gov/vocabulary/relators/edt 702 $aErdmann$b Rainer$4edt$4http://id.loc.gov/vocabulary/relators/edt 906 $aBOOK 912 $a9910298628903321 996 $aAdvanced Photon Counting$92069843 997 $aUNINA