LEADER 06199nam 22007095 450 001 9910298613303321 005 20200630053455.0 010 $a1-4939-2346-3 024 7 $a10.1007/978-1-4939-2346-5 035 $a(CKB)3710000000343628 035 $a(EBL)1973888 035 $a(SSID)ssj0001424263 035 $a(PQKBManifestationID)11748687 035 $a(PQKBTitleCode)TC0001424263 035 $a(PQKBWorkID)11362987 035 $a(PQKB)11402685 035 $a(DE-He213)978-1-4939-2346-5 035 $a(MiAaPQ)EBC1973888 035 $a(PPN)183518691 035 $a(EXLCZ)993710000000343628 100 $a20150128d2015 u| 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aDetection and Typing Strategies for Pathogenic Escherichia coli /$fby Lucia Rivas, Glen E. Mellor, Kari Gobius, Narelle Fegan 205 $a1st ed. 2015. 210 1$aNew York, NY :$cSpringer New York :$cImprint: Springer,$d2015. 215 $a1 online resource (114 p.) 225 1 $aSpringerBriefs in Food, Health, and Nutrition,$x2197-571X 300 $aDescription based upon print version of record. 311 $a1-4939-2345-5 320 $aIncludes bibliographical references. 327 $aChapter 1 Introduction to Pathogenic Escherichia coli -- 1.1 Pathotypes of Escherichia coli causing diarrhoeal diseases -- 1.2 Escherichia coli -- 1.3 Pathogenic types of E. coli -- 1.4 Shiga toxin-producing and Enterohemorrhagic E. coli -- 1.4.1    Virulence determinants of Shiga-toxin producing E. coli -- 1.4.2 Shiga-toxin producing E. coli-mediated disease -- 1.4.3 Epidemiology of Shiga toxin-producing E. coli -- 1.4.4    Ecology of Shiga-toxin producing E. coli -- 1.4.5 Transmission of Shiga-toxin producing E. coli -- 1.4.6 Shiga-toxin producing E. coli occurrence in foods -- 1.5 Enterotoxigenic E. coli -- 1.6 Enteropathogenic E. coli -- 1.7 Enteroinvasive E. coli -- 1.8 Enteroaggregative and Diffusely Adherent E. coli..-1.9 References -- Chapter 2 Isolation and detection of pathogenic Escherichia coli in foods -- 2.1 Introduction -- 2.2 General method of isolation for E. coli -- 2.3 Shiga toxin-producing E. coli -- 2.3.1 Culture and isolation of Shiga toxin-producing E. coli -- 2.3.2    Molecular detection of Shiga toxin-producing E. coli -- 2.4 Enumeration of Shiga toxin-producing E.coli -- 2.5 Immunological detection methods for pathogenic E. coli -- 2.6 Cell culture assays used for pathogenic E. coli -- 2.6.1 Cell cytotoxicity assay for Shiga toxin-producing E. coli -- 2.6.2    Cell adherence assays -- 2.7 Enteroinvasive E. coli -- 2.8 Enterotoxigenic E. coli -- 2.9 Enteropathogenic E. coli -- 2.10     Enteroaggregative E. coli and Diffusely Adherent E. coli -- 2.11 References -- Chapter 3 Typing and Subtyping methods for pathogenic Escherichia coli -- 3.1 Introduction -- 3.2 Biochemical profiling -- 3.3 Serotyping -- 3.4 Phage typing -- 3.5 Multilocus enzyme electrophoresis -- 3.6 Multilocus sequence typing -- 3.7 Pulsed-field gel electrophoresis -- 3.8 Multiple-locus variable number tandem repeat analysis -- 3.9 Repetitive element palindromic polymerase chain reaction -- 3.10 Random amplified polymorphic DNA -- 3.11 Shiga toxin subtypes and bacteriophage insertion sites -- 3.12 Lineage specific polymorphism analysis -- 3.13 Whole genome sequencing -- 3.14 -- References -- Chapter 4 Emerging and future trends and technologies for the detection and typing of Escherichia coli -- 4.1 Future trends in foodborne illness and E. coli -- 4.2 Future trends in the detection and typing of pathogens -- 4.2.1 Microarrays -- 4.2.2 Matrix Assisted Laser-Desorption Ionization-Time-Of-Flight Mass Spectrometry -- 4.2.3 Nanotechnology -- 4.2.4 Next-generating sequencing of the whole bacterial genome -- 4.3 References. 330 $aThis Brief will review the methods that are currently available for the detection, isolation, and typing of pathogenic E. coli with a particular focus on foodborne diseases caused by the Shiga toxigenic E. coli group, which have been implicated in a number of significant outbreaks in recent years. Pathogenic forms of E. coli can cause a variety of diarrheal diseases in hosts due to the presence of specific colonization and virulence factors, and pathogenicity-associated genes, which are generally not present in other E. coli. Six pathotypes of pathogenic E. coli are recognized (Shiga toxigenic E. coli, Enteropathogenic E. coli, Enterotoxigenic E. coli, Enteroinvasive E. coli, Enteroaggregative E. coli and Diffusely Adherent E. coli) and certain strains among these groups are major public health concerns due to the severity of disease that they can cause. Methods to detect and isolate these pathogens from a variety of sources are constantly evolving. In addition, the accumulation of knowledge on these pathogens allows for improved intervention strategies. 410 0$aSpringerBriefs in Food, Health, and Nutrition,$x2197-571X 606 $aFood?Biotechnology 606 $aBacteriology 606 $aMicrobiology 606 $aFood Science$3https://scigraph.springernature.com/ontologies/product-market-codes/C15001 606 $aBacteriology$3https://scigraph.springernature.com/ontologies/product-market-codes/L23012 606 $aApplied Microbiology$3https://scigraph.springernature.com/ontologies/product-market-codes/C12010 615 0$aFood?Biotechnology. 615 0$aBacteriology. 615 0$aMicrobiology. 615 14$aFood Science. 615 24$aBacteriology. 615 24$aApplied Microbiology. 676 $a54 676 $a579.3 676 $a641.3 676 $a660.62 676 $a664 700 $aRivas$b Lucia$4aut$4http://id.loc.gov/vocabulary/relators/aut$0925440 702 $aMellor$b Glen E$4aut$4http://id.loc.gov/vocabulary/relators/aut 702 $aGobius$b Kari$4aut$4http://id.loc.gov/vocabulary/relators/aut 702 $aFegan$b Narelle$4aut$4http://id.loc.gov/vocabulary/relators/aut 906 $aBOOK 912 $a9910298613303321 996 $aDetection and Typing Strategies for Pathogenic Escherichia coli$92077938 997 $aUNINA