LEADER 04699nam 22006975 450 001 9910298579603321 005 20251116195418.0 010 $a3-319-73405-9 024 7 $a10.1007/978-3-319-73405-7 035 $a(CKB)4100000002892254 035 $a(MiAaPQ)EBC5357938 035 $a(DE-He213)978-3-319-73405-7 035 $a(PPN)22554864X 035 $a(EXLCZ)994100000002892254 100 $a20180302d2018 u| 0 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aFluorescent Tools for Imaging Oxidative Stress in Biology /$fby Amandeep Kaur 205 $a1st ed. 2018. 210 1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2018. 215 $a1 online resource (265 pages) $cillustrations 225 1 $aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053 300 $a"Doctoral thesis accepted by the University of Sydney, Australia." 311 08$a3-319-73404-0 327 $aIntroduction -- Flavin based redox probes -- FRET based ratiometric redox probes -- Mitochondrially-targeted ratiometric redox probes -- Nicotinamide based ratiometric probes -- In cellulo studies -- Ex vivo studies -- Non-mammalian systems -- Conclusions -- Experimental Methods. . 330 $aThis thesis advances the long-standing challenge of measuring oxidative stress and deciphering its underlying mechanisms, and also outlines the advantages and limitations of existing design strategies. It presents a range of approaches for the chemical synthesis of fluorescent probes that detect reversible changes in cellular oxidative stress. The ability to visualise cellular processes in real-time is crucial to understanding disease development and streamline treatment, and this can be achieved using fluorescent tools that can sense reversible disturbances in cellular environments during pathogenesis. The perturbations in cellular redox state are of particular current interest in medical research, since oxidative stress is implicated in the pathogenesis of a number of diseases. The book investigates different strategies used to achieve ratiometric fluorescence output of the reversible redox probes, which nullify concentration effects associated with intensity-based probes. It also describes suitable approaches to target these probes to specific cellular organelles, thereby enabling medical researchers to visualise sub-cellular oxidative stress levels, and addressing the typically poor uptake of chemical tools into biological studies. In total it reports on four new probes that are now being used by over twenty research groups around the globe, and two of which have been commercialised. The final chapters of this thesis demonstrate successful applications of the sensors in a variety of biological systems ranging from prokaryotes to mammalian cells and whole organisms. The results described clearly indicate the immense value of collaborative, cross-disciplinary research. 410 0$aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053 606 $aAnalytical chemistry 606 $aOxidative stress 606 $aBiophysics 606 $aBiophysics 606 $aBiomedical engineering 606 $aChemistry, Physical and theoretical 606 $aAnalytical Chemistry$3https://scigraph.springernature.com/ontologies/product-market-codes/C11006 606 $aOxidative Stress$3https://scigraph.springernature.com/ontologies/product-market-codes/L16070 606 $aBiological and Medical Physics, Biophysics$3https://scigraph.springernature.com/ontologies/product-market-codes/P27008 606 $aBiomedical Engineering/Biotechnology$3https://scigraph.springernature.com/ontologies/product-market-codes/B24000 606 $aPhysical Chemistry$3https://scigraph.springernature.com/ontologies/product-market-codes/C21001 615 0$aAnalytical chemistry. 615 0$aOxidative stress. 615 0$aBiophysics. 615 0$aBiophysics. 615 0$aBiomedical engineering. 615 0$aChemistry, Physical and theoretical. 615 14$aAnalytical Chemistry. 615 24$aOxidative Stress. 615 24$aBiological and Medical Physics, Biophysics. 615 24$aBiomedical Engineering/Biotechnology. 615 24$aPhysical Chemistry. 676 $a616.39 700 $aAmandeep Kaur$4aut$4http://id.loc.gov/vocabulary/relators/aut$01879100 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910298579603321 996 $aFluorescent Tools for Imaging Oxidative Stress in Biology$94498461 997 $aUNINA