LEADER 04155nam 22006135 450 001 9910298452703321 005 20200706112755.0 010 $a3-319-19497-6 024 7 $a10.1007/978-3-319-19497-4 035 $a(CKB)3710000000460496 035 $a(EBL)4178275 035 $a(SSID)ssj0001546890 035 $a(PQKBManifestationID)16141447 035 $a(PQKBTitleCode)TC0001546890 035 $a(PQKBWorkID)14795880 035 $a(PQKB)11601447 035 $a(DE-He213)978-3-319-19497-4 035 $a(MiAaPQ)EBC3564457 035 $a(MiAaPQ)EBC4178275 035 $a(PPN)188457232 035 $a(EXLCZ)993710000000460496 100 $a20150806d2015 u| 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aProteases in Apoptosis: Pathways, Protocols and Translational Advances /$fedited by Kakoli Bose 205 $a1st ed. 2015. 210 1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2015. 215 $a1 online resource (258 p.) 300 $aDescription based upon print version of record. 311 $a3-319-19496-8 320 $aIncludes bibliographical references at the end of each chapters and index. 327 $aApoptosis: Pathways, Molecules and Beyond -- Caspases - key players in apoptosis -- Calpains and Granzymes: non-caspase proteases in cell death -- Cathepsins and HtrAs - multitasking proteases in programmed cell death -- Proteases in Apoptosis: Protocols and Methods -- Preclinical Animal Model and Non-invasive Imaging in Apoptosis. 330 $aThis book provides a comprehensive overview of the proteases involved in programmed cell death. It presents a focused yet extensive discussion on proteolytic enzymes such as caspases, HtrAs, granzymes, calpains and cathepsins as well as laboratory protocols related to enzymology and apoptosis. Mouse model systems and non-invasive imaging techniques in apoptosis-related diseases such as cancer and neurodegeneration are also covered in this book. While slowly unravelling the complexities of apoptosis in chapter one, the next three chapters individually elaborate on different classes of proteases that play key roles in the initiation, progression and execution of programmed cell death. The last two chapters complete this discussion by describing different laboratory methodologies and therapeutic advances involving apoptotic proteases. Protocols portraying in vitro and ex vivo colorimetric and fluorescence-based enzyme kinetic studies as well as cell death assays are explained in the fifth chapter. Preclinical in vivo models and non-invasive imaging in apoptosis to understand the complexities of disease progression and their contribution toward therapeutics are recounted in the last chapter. The book spans topics related to both fundamental and applied biology. It would therefore be equally appealing and informative to scientists working in the field of apoptosis and those who are investigating mechanisms of proteases and enzymes in general. The protocols would certainly benefit both graduate and undergraduate students working in the related fields and provide useful leads for drug design to translational biologists involved in neurodegeneration and cancer research. 606 $aApoptosis 606 $aMedicine 606 $aMedical biochemistry 606 $aApoptosis$3https://scigraph.springernature.com/ontologies/product-market-codes/L16060 606 $aBiomedicine, general$3https://scigraph.springernature.com/ontologies/product-market-codes/B0000X 606 $aMedical Biochemistry$3https://scigraph.springernature.com/ontologies/product-market-codes/H35005 615 0$aApoptosis. 615 0$aMedicine. 615 0$aMedical biochemistry. 615 14$aApoptosis. 615 24$aBiomedicine, general. 615 24$aMedical Biochemistry. 676 $a570 702 $aBose$b Kakoli$4edt$4http://id.loc.gov/vocabulary/relators/edt 906 $aBOOK 912 $a9910298452703321 996 $aProteases in Apoptosis: Pathways, Protocols and Translational Advances$92526664 997 $aUNINA