LEADER 04071nam 22005655 450 001 9910298420103321 005 20200705145430.0 010 $a3-319-77982-6 024 7 $a10.1007/978-3-319-77982-9 035 $a(CKB)4100000004243831 035 $a(DE-He213)978-3-319-77982-9 035 $a(MiAaPQ)EBC5403420 035 $a(PPN)22740646X 035 $a(EXLCZ)994100000004243831 100 $a20180526d2018 u| 0 101 0 $aeng 135 $aurnn|008mamaa 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 14$aThe ABCs of Gene Cloning /$fby Dominic W. S. Wong 205 $a3rd ed. 2018. 210 1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2018. 215 $a1 online resource (XIX, 257 p. 172 illus., 1 illus. in color.) 311 $a3-319-77762-9 327 $aFundamentals of Genetic Processes -- Introductory Concepts -- Structures of Nucleic Acids -- Structures of Proteins -- The Genetic Process -- Organization of Genes -- Reading The Nucleotide Sequence of a Gene -- Techniques and Strategies of Gene Cloning -- Enzymes Used in Cloning -- Techniques Used in Cloning -- Cloning Vectors for Introducing Genes into Host Cells -- Transformation -- Isolating Genes for Cloning -- Impact of Gene Cloning -- Improving Tomato Quality by Antisense Rna -- Transgenic Crops Engineered with Insecticidal Activity -- Transgenic Crops Conferred with Herbicide Resistance -- Growth Enhancement in Transgenic Fish -- Impact of Gene Cloning -- Microbial Production of Recombinant Human Insulin -- Finding Disease-Causing Genes -- Human Gene Therapy -- Gene Targeting -- DNA Typing -- Transpharmers - Bioreactors for Pharmaceutical Products -- Animal Cloning -- Human Genome Sequencing -- Manipulating Gene Vector Constructs. 330 $aClear and concise, this easy-to-use book offers an introductory course on the language of gene cloning, covering microbial, plant, and mammalian systems. It presents the nuts and bolts of gene cloning in a well-organized and accessible manner. Part I of this book outlines the essentials of biology and genetics relevant to the concept of gene cloning. Part II describes common techniques and approaches of gene cloning, ranging from the basic mechanics of DNA manipulation, vector systems, process transformation, to gene analysis. Part III & IV present application technologies of major impact in agriculture, biomedicine, and related areas. The ABCs of Gene Cloning, Third Edition contains updates including a tutorial chapter on gene-vector construction, methodologies on exome sequencing in finding disease genes, revised topics on gene therapy and whole genome sequencing, new developments for gene targeting and genome editing, as well as the current state of next generation sequencing. With more than 140 illustrations, this new edition provides an invaluable text for students and anyone who have interest in gaining proficiency in reading and speaking the language of gene cloning. 606 $aHuman genetics 606 $aFood?Biotechnology 606 $aPlant physiology 606 $aZoology 606 $aHuman Genetics$3https://scigraph.springernature.com/ontologies/product-market-codes/B12008 606 $aFood Science$3https://scigraph.springernature.com/ontologies/product-market-codes/C15001 606 $aPlant Physiology$3https://scigraph.springernature.com/ontologies/product-market-codes/L33020 606 $aZoology$3https://scigraph.springernature.com/ontologies/product-market-codes/L25007 615 0$aHuman genetics. 615 0$aFood?Biotechnology. 615 0$aPlant physiology. 615 0$aZoology. 615 14$aHuman Genetics. 615 24$aFood Science. 615 24$aPlant Physiology. 615 24$aZoology. 676 $a611.01816 676 $a599.935 700 $aWong$b Dominic W. S$4aut$4http://id.loc.gov/vocabulary/relators/aut$0768469 906 $aBOOK 912 $a9910298420103321 996 $aThe ABCs of Gene Cloning$92529404 997 $aUNINA