LEADER 05187nam 22006255 450 001 9910298419003321 005 20200629232532.0 010 $a981-10-8186-7 024 7 $a10.1007/978-981-10-8186-6 035 $a(CKB)4100000002892668 035 $a(MiAaPQ)EBC5334714 035 $a(DE-He213)978-981-10-8186-6 035 $a(PPN)225549190 035 $a(EXLCZ)994100000002892668 100 $a20180330d2018 u| 0 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aBasic Concepts in Clinical Biochemistry: A Practical Guide$b[electronic resource] /$fby Vijay Kumar, Kiran Dip Gill 205 $a1st ed. 2018. 210 1$aSingapore :$cSpringer Singapore :$cImprint: Springer,$d2018. 215 $a1 online resource (174 pages) 311 $a981-10-8185-9 320 $aIncludes bibliographical references and index. 327 $aCommon clinical laboratory hazards and waste disposal -- Blood collection and preservation -- Quality Control in laboratory -- Automation in clinical laboratory -- Photometry-Colorimeter and Spectrophotometer -- Preparation of general laboratory solutions and buffers -- Examination of urine for normal constituents -- To perform qualitative tests for urinary proteins -- To determine the quantity of proteins in urine sample using Biuret reaction -- To estimate the amount of total protein and albumin in serum and to find A/G ratio -- To perform qualitative test for reducing substances in urine -- Quantitative analysis of reducing sugars in urine -- Estimation of blood glucose levels by Glucose Oxidase method -- Estimation of blood glucose levels by Folin and Wu method -- To perform Glucose Tolerance Test -- Estimation of urea in serum and urine -- To determine urea clearance -- To estimate creatinine level in serum and urine by Jaffe?s reaction -- To determine creatinine clearance -- To determine the uric acid concentration in serum and urine -- Estimation of total calcium in serum and urine -- Estimation of inorganic phosphorus in serum and urine -- To estimate the amount of total cholesterol in serum -- To estimate total and direct bilirubin in serum -- To determine Alanine and  Aspartate Transaminases activity in serum -- To estimate the activity of alkaline phosphatase in serum -- To estimate the activity of acid phosphatase in serum -- To determine serum and urinary amylase activity -- To estimate the activity of lipase in serum -- Qualitative analysis of ketone bodies in urine -- Qualitative test for bile pigments and urobilinogen in urine -- Determination of total lactate dehydrogenase activity in serum -- To measure the activity of Creatine kinase-MB and Total Creatine kinase in serum -- Analysis of Cerebrospinal Fluid for proteins and sugars -- To analyze lipid profile from given serum sample -- To determine sodium and potassium in serum by using Flame photometry -- To perform Radioimmunoassay -- To perform Enzyme linked Immunosorbant assay -- Some important case studies. 330 $aThis book is a practical guidebook in biochemistry, for medical as well as life sciences' students. The book covers reference values, sample collection procedure and detailed protocol to perform experiments. Each experiment starts with a brief introduction of the protocol, followed by specimen requirements and procedure. The procedures are presented in a very lucid manner and discuss details of calculations and clinical interpretations,The book is divided into 29 chapters, It offers references, general guidelines and abbreviations and provides principles and procedures of clinical biochemistry tests, along with their diagnostic importance. 606 $aBiomedical engineering 606 $aBiochemistry 606 $aMedical biochemistry 606 $aBiotechnology 606 $aLaboratory medicine 606 $aBiomedical Engineering/Biotechnology$3https://scigraph.springernature.com/ontologies/product-market-codes/B24000 606 $aBiochemistry, general$3https://scigraph.springernature.com/ontologies/product-market-codes/L14005 606 $aMedical Biochemistry$3https://scigraph.springernature.com/ontologies/product-market-codes/H35005 606 $aBiotechnology$3https://scigraph.springernature.com/ontologies/product-market-codes/C12002 606 $aLaboratory Medicine$3https://scigraph.springernature.com/ontologies/product-market-codes/B15007 615 0$aBiomedical engineering. 615 0$aBiochemistry. 615 0$aMedical biochemistry. 615 0$aBiotechnology. 615 0$aLaboratory medicine. 615 14$aBiomedical Engineering/Biotechnology. 615 24$aBiochemistry, general. 615 24$aMedical Biochemistry. 615 24$aBiotechnology. 615 24$aLaboratory Medicine. 676 $a616.07 700 $aKumar$b Vijay$4aut$4http://id.loc.gov/vocabulary/relators/aut$0640466 702 $aGill$b Kiran Dip$4aut$4http://id.loc.gov/vocabulary/relators/aut 906 $aBOOK 912 $a9910298419003321 996 $aBasic Concepts in Clinical Biochemistry: A Practical Guide$92536230 997 $aUNINA