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010   $a3-319-98071-8
024 7 $a10.1007/978-3-319-98071-3
035   $a(CKB)4100000007127536
035   $a(MiAaPQ)EBC5592914
035   $a(DE-He213)978-3-319-98071-3
035   $a(PPN)232472556
035   $a(EXLCZ)994100000007127536
100   $a20181108d2018      u|         0
101 0 $aeng
135   $aurcnu||||||||
181   $ctxt$2rdacontent
182   $cc$2rdamedia
183   $acr$2rdacarrier
200 10$aFlow Cytometry Basics for the Non-Expert /$fby Christine Goetz, Christopher Hammerbeck, Jody Bonnevier
205   $a1st ed. 2018.
210  1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2018.
215   $a1 online resource (xvii, 219 pages)
225 1 $aTechniques in Life Science and Biomedicine for the Non-Expert,$x2367-1114
311   $a3-319-98070-X 
327   $a1. Flow Cytometry: Definition, History, and Uses in Biotechnology -- 2. Physics of a Flow Cytometer -- 3. The Language of Flow Cytometry and Experimental Setup -- 4. Fluorochrome Choices for Flow Cytometry -- 5. Compensation -- 6. Primary and Seconardy Antibodies and Their Use in Flow Cytometry -- 7. Experimental Considerations with Data Sets as Examples -- 8. Cell Enrichment -- 9. Surface and Intracellular Staining Protocols for Flow Cytometry -- 10. Troubleshooting.
330   $aFlow cytometry allows for the measurement of different aspects of a cell or particles in real time. Applications include quantifying various types of T cells to determine the presence of various immune disorders, such as whether HIV has progressed to AIDS, studying the dynamics of immune signaling in cells to detect the suppression of normal immune function, and analyzing biopsy specimens for tumors, to aid in cancer diagnosis and prognosis. In addition to simplifying the technique and using vocabulary accessible to those not trained in cell biology and immunology, the book includes the following 4 unqiue features: 1) Tips on how to set up an experiment for flow cytometry optimially 2) An expanded data set of flow staining including multiparameter flow cytometry 3) Detailed staining protocols for flow cytometry (ex. protocols optimized for transcription factors, secreted cytokines, phospho-antibodies, and surface antigens) 4) Section devoted to antibody development and conjugation.
410  0$aTechniques in Life Science and Biomedicine for the Non-Expert,$x2367-1114
606   $aImmunoglobulins
606   $aDiagnosis, Laboratory
606   $aAntibodies$3https://scigraph.springernature.com/ontologies/product-market-codes/B14010
606   $aLaboratory Medicine$3https://scigraph.springernature.com/ontologies/product-market-codes/B15007
615  0$aImmunoglobulins.
615  0$aDiagnosis, Laboratory.
615 14$aAntibodies.
615 24$aLaboratory Medicine.
676   $a574.87028
700   $aGoetz$b Christine$4aut$4http://id.loc.gov/vocabulary/relators/aut$01059859
702   $aHammerbeck$b Christopher$4aut$4http://id.loc.gov/vocabulary/relators/aut
702   $aBonnevier$b Jody$4aut$4http://id.loc.gov/vocabulary/relators/aut
906   $aBOOK
912   $a9910298407103321
996   $aFlow Cytometry Basics for the Non-Expert$92508889
997   $aUNINA