LEADER 03943nam 22007215 450 001 9910298329003321 005 20200703091337.0 010 $a3-0348-0856-9 024 7 $a10.1007/978-3-0348-0856-9 035 $a(CKB)3710000000218675 035 $a(EBL)1802959 035 $a(SSID)ssj0001338289 035 $a(PQKBManifestationID)11735551 035 $a(PQKBTitleCode)TC0001338289 035 $a(PQKBWorkID)11337752 035 $a(PQKB)11429357 035 $a(MiAaPQ)EBC1802959 035 $a(DE-He213)978-3-0348-0856-9 035 $a(PPN)180627473 035 $a(EXLCZ)993710000000218675 100 $a20140805d2014 u| 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aFluorescent Methods for Molecular Motors /$fedited by Christopher P. Toseland, Natalia Fili 205 $a1st ed. 2014. 210 1$aBasel :$cSpringer Basel :$cImprint: Springer,$d2014. 215 $a1 online resource (306 p.) 225 1 $aExperientia Supplementum,$x1664-431X ;$v105 300 $aDescription based upon print version of record. 311 $a1-322-13356-5 311 $a3-0348-0855-0 320 $aIncludes bibliographical references at the end of each chapters. 327 $aFluorescence and Labelling: How to choose and what to do -- Fluorescent biosensors: design and application to motor proteins -- Rapid Reaction Kinetic Techniques -- Fluorescence to study the ATPase mechanism of Motor Proteins -- Use of pyrene labelled actin to probe actin myosin interactions; kinetic and equilibrium studies -- Fluorescent methods to study transcription initiation and transition into elongation -- Single-molecule and single-particle imaging of molecular motors in vitro and in vivo -- Fluorescence methods in the investigation of the DEAD-box helicase mechanism -- Use of Fluorescent Techniques to Study the In Vitro Movement of Myosins -- Fluorescence Tracking of Motor proteins in vitro -- Measuring Transport of Motor Cargos -- Measuring two at the same time: Combining Magnetic Tweezers with Single-Molecule FRET -- Using fluorescence to study actomyosin in yeasts. 330 $aThis book focuses on the application of fluorescence to study motor proteins (myosins, kinesins, DNA helicases and RNA polymerases). It is intended for a large community of biochemists, biophysicists and cell biologists who study a diverse collection of motor proteins. It can be used by researchers to gain an insight into their first experiments, or by experienced researchers who are looking to expand their research to new areas. Each chapter provides valuable advice for executing the experiments, along with detailed background knowledge in order to develop own experiments. 410 0$aExperientia Supplementum,$x1664-431X ;$v105 606 $aProteins  606 $aCell biology 606 $aBiophysics 606 $aBiological physics 606 $aProtein Science$3https://scigraph.springernature.com/ontologies/product-market-codes/L14040 606 $aCell Biology$3https://scigraph.springernature.com/ontologies/product-market-codes/L16008 606 $aBiological and Medical Physics, Biophysics$3https://scigraph.springernature.com/ontologies/product-market-codes/P27008 615 0$aProteins . 615 0$aCell biology. 615 0$aBiophysics. 615 0$aBiological physics. 615 14$aProtein Science. 615 24$aCell Biology. 615 24$aBiological and Medical Physics, Biophysics. 676 $a574.19245 676 $a572.64 702 $aToseland$b Christopher P$4edt$4http://id.loc.gov/vocabulary/relators/edt 702 $aFili$b Natalia$4edt$4http://id.loc.gov/vocabulary/relators/edt 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910298329003321 996 $aFluorescent Methods for Molecular Motors$92539462 997 $aUNINA