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010   $a94-017-8819-7
024 7 $a10.1007/978-94-017-8819-9
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035   $a(EBL)1731570
035   $a(OCoLC)881443479
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035   $a(MiAaPQ)EBC1731570
035   $a(DE-He213)978-94-017-8819-9
035   $a(iGPub)SPNA0035491
035   $a(PPN)17878530X
035   $a(EXLCZ)993710000000114553
100   $a20140519d2014      u|         0
101 0 $aeng
135   $aur|n|---|||||
181   $ctxt
182   $cc
183   $acr
200 10$aStudies of Pluripotency in Embryonic Stem Cells and Induced Pluripotent Stem Cells /$fby Xiaoyang Zhao
205   $a1st ed. 2014.
210  1$aDordrecht :$cSpringer Netherlands :$cImprint: Springer,$d2014.
215   $a1 online resource (104 p.)
225 1 $aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053
300   $a"Doctoral thesis accepted by University of Chinese Academy of Sciences, Beijing, China."
311   $a94-017-8818-9 
320   $aIncludes bibliographical references at the end of each chapters.
327   $aAbstract -- Introduction -- Establishment of highly efficient somatic cell reprogramming system to generate iPSC lines- Establishment of highly efficient somatic cell reprogramming system and establishment of iPSC lines -- Pluripotency of iPSC and underlining mechanism -- Developmental potential of mouse iPSC -- Conclusions.
330   $aStem cells have the ability to differentiate into all types of cells within the body, thus have great therapeutic potential for regenerative medicine to treat complicated disorders, like Parkinson?s disease and spinal cord injury. There will also be many applications in drug development. However, several roadblocks, such as safety issues and low efficiency of pluripotent stem cell (PSC) line derivation need to be resolved before their clinical application. This thesis focuses on these two areas, so as to find methods to overcome the limitation. It covers deriving embryonic stem cells (ESCs) from several different species, and reports an efficient system to generate induced pluripotent stem cells (iPSCs), and the first iPSC mice in the world. The results in this thesis confirm that somatic cells can be fully reprogrammed with the four Yamanaka factors. In addition, we have found that the Dlk1-Dio3 region can be a potential molecular marker to distinguish the fully reprogrammed iPSCs from partially reprogrammed ones. All of these results will help improve the safety of PSCs in the clinical applications, and increase the current low induction efficiency of their production.
410  0$aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053
606   $aStem cells
606   $aCell culture
606   $aRegenerative medicine
606   $aTissue engineering
606   $aDevelopmental biology
606   $aStem Cells$3https://scigraph.springernature.com/ontologies/product-market-codes/L16010
606   $aCell Culture$3https://scigraph.springernature.com/ontologies/product-market-codes/L16020
606   $aRegenerative Medicine/Tissue Engineering$3https://scigraph.springernature.com/ontologies/product-market-codes/L16080
606   $aDevelopmental Biology$3https://scigraph.springernature.com/ontologies/product-market-codes/L18000
615  0$aStem cells.
615  0$aCell culture.
615  0$aRegenerative medicine.
615  0$aTissue engineering.
615  0$aDevelopmental biology.
615 14$aStem Cells.
615 24$aCell Culture.
615 24$aRegenerative Medicine/Tissue Engineering.
615 24$aDevelopmental Biology.
676   $a571.6
700   $aZhao$b Xiaoyang$4aut$4http://id.loc.gov/vocabulary/relators/aut$01058752
906   $aBOOK
912   $a9910298320803321
996   $aStudies of Pluripotency in Embryonic Stem Cells and Induced Pluripotent Stem Cells$92502161
997   $aUNINA