LEADER 04005nam 22007095 450 001 9910298293603321 005 20220407175353.0 010 $a1-4899-7507-1 024 7 $a10.1007/978-1-4899-7507-2 035 $a(CKB)3710000000277376 035 $a(EBL)1964990 035 $a(OCoLC)894893243 035 $a(SSID)ssj0001385805 035 $a(PQKBManifestationID)11994425 035 $a(PQKBTitleCode)TC0001385805 035 $a(PQKBWorkID)11341299 035 $a(PQKB)10095070 035 $a(DE-He213)978-1-4899-7507-2 035 $a(MiAaPQ)EBC1964990 035 $a(PPN)183087380 035 $a(EXLCZ)993710000000277376 100 $a20141107d2015 u| 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aAtlas of human pluripotent stem cells in culture /$fby Lyn Healy, Ludmila Ruban 205 $a1st ed. 2015. 210 1$aNew York, NY :$cSpringer US :$cImprint: Springer,$d2015. 215 $a1 online resource (216 p.) 300 $aDescription based upon print version of record. 311 $a1-4899-7506-3 320 $aIncludes bibliographical references and index. 327 $a1 Before We Begin: An Introduction to the Culture of Pluripotent Cells -- 2 Mouse and Human Fibroblasts -- 3 Inactivated Mouse and Human Fibroblasts -- 4 Human Embryonic Stem Cells -- 5 Human Induced Pluripotent Stem Cells on Feeders -- 6 Pluripotent Cell Lines Grown on Different Substrates and Surfaces -- 7 Passaging Pluripotent Stem Cells -- 8 Characteristic Staining Patterns of Undifferentiated and Differentiated Pluripotent Stem Cells -- 9 Derivation of Induced Pluripotent Stem Cells -- 10 Culture Adaptation and Abnormal Cultures -- 11 Infection -- 12 Miscellaneous Cell Types and Cell Lines of Interest. 330 $aThis lavishly-illustrated, authoritative atlas explores the intricate art of culturing human pluripotent stem cells. Twelve chapters ? containing more than 280 color illustrations ? cover a variety of topics in pluripotent stem cell culturing including mouse and human fibroblasts, human embryonic stem cells and induced pluripotent stem cells, characteristic staining patterns, and abnormal cultures, among others. Atlas of Human Pluripotent Stem Cells in Culture is a comprehensive collection of illustrated techniques complemented by informative and educational captions examining what good quality cells look like and how they behave in various environments. Examples of perfect cultures are compared side-by-side to less-than-perfect and unacceptable examples of human embryonic and induced pluripotent stem cell colonies. This detailed and thorough atlas is an invaluable resource for researchers, teachers, and students who are interested in or working with stem cell culturing. . 606 $aStem cells 606 $aCell culture 606 $aBiochemical engineering 606 $aCytology 606 $aStem Cells$3https://scigraph.springernature.com/ontologies/product-market-codes/L16010 606 $aCell Culture$3https://scigraph.springernature.com/ontologies/product-market-codes/L16020 606 $aBiochemical Engineering$3https://scigraph.springernature.com/ontologies/product-market-codes/C12029 606 $aCell Biology$3https://scigraph.springernature.com/ontologies/product-market-codes/L16008 615 0$aStem cells. 615 0$aCell culture. 615 0$aBiochemical engineering. 615 0$aCytology. 615 14$aStem Cells. 615 24$aCell Culture. 615 24$aBiochemical Engineering. 615 24$aCell Biology. 676 $a570 676 $a571.6 676 $a571.638 676 $a660.63 700 $aHealy$b Lyn$4aut$4http://id.loc.gov/vocabulary/relators/aut$01064851 702 $aRuban$b Ludmila$4aut$4http://id.loc.gov/vocabulary/relators/aut 906 $aBOOK 912 $a9910298293603321 996 $aAtlas of Human Pluripotent Stem Cells in Culture$92541210 997 $aUNINA