LEADER 05678nam 22007815 450 001 9910298282803321 005 20200704082542.0 010 $a3-319-10142-0 024 7 $a10.1007/978-3-319-10142-2 035 $a(CKB)3710000000269473 035 $a(EBL)1967205 035 $a(OCoLC)894509236 035 $a(SSID)ssj0001372146 035 $a(PQKBManifestationID)11978652 035 $a(PQKBTitleCode)TC0001372146 035 $a(PQKBWorkID)11319442 035 $a(PQKB)10683247 035 $a(DE-He213)978-3-319-10142-2 035 $a(MiAaPQ)EBC1967205 035 $a(PPN)182096459 035 $a(EXLCZ)993710000000269473 100 $a20141028d2015 u| 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aGenetic Transformation Systems in Fungi, Volume 1 /$fedited by Marco A. van den Berg, Karunakaran Maruthachalam 205 $a1st ed. 2015. 210 1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2015. 215 $a1 online resource (310 p.) 225 1 $aFungal Biology,$x2198-7777 300 $aDescription based upon print version of record. 311 $a3-319-10141-2 320 $aIncludes bibliographical references and index. 327 $aFungal Transformation: From Protoplasts to Targeted Recombination Systems -- Protoplast Transformation for Genome Manipulation in Fungi -- Trichoderma Transformation Methods -- Transformation of Mucor circinelloides f. lusitanicus Protoplasts -- Transformation of Saccharomyces cerevisiae: Spheroplast Method -- Electroporation Mediated DNA Transformation of Filamentous Fungi -- Chemical Transformation of Candida albicans -- Electroporation of Pichia pastoris -- Insertional Mutagenesis of the Flavinogenic Yeast Candida famata (Candida flareli) -- Biolistic Transformation for Delivering DNA into the Mitochondria -- Bolistic Transformation of Candida glabrata for Homoplasmic Mitochondrial Genome Transformants -- Use of the Biolistic Particle Delivery System to Transform Fungal Genomes -- Transformation of Zygomycete Mortierella alpina Using Biolistic Particle Bombardment -- Agrobacterium tumefaciens-Mediated Transformation -- Agrobacterium tumefaciens-Mediated Transformation of Pucciniomycotina Red Yeasts -- Glass-bead and Agrobacterium-mediated Genetic Transformation of Fusarium oxysporum -- High Efficiency DNA Transformation of Saccharomyces cerevisiae with the LiAc/SS-DNA/PEG Method -- Transformation of Intact Cells of Saccharomyces cerevisiae: Lithium Methods and Possible Underlying Mechanism -- Transformation of Lithium Acetate-treated Neurospora crassa -- Application of Novel Polymeric Carrier of Plasmid DNA for Transformation of Yeast Cells -- Transformation of Fungi Using Shock Waves -- Pathways and Mechanisms of Yeast Competence: A New Frontier of Yeast Genetics -- Evaluation of Competence Phenomenon of Yeast Saccharomyces cerevisiae by Lipophilic Cations Accumulation and FT-IR Spectroscopy. Relation of Competence to Cell Cycle -- Recombination and Gene Targeting in Neurospora -- Efficient Generation of Aspergillus niger Knock Out strains by Combining NHEJ Mutants and a Split Marker Approach -- REMI in Molecular Fungal Biology -- TALEN-Based Genome Editing in Yeast. 330 $aSeveral different transformation techniques have been developed over the years and readily shown to be decisive methods in fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examples used in laboratories worldwide. Not only will this text provide a detailed background on applications in industrial and pharmaceutical relevant microbes, but also the importance of fungal pathogens in agricultural production (Phytophthora and Botrytis) and mammalian infection (Penicillium marneffei and Candida). Genetic Transformation Systems in Fungi, Volume 1 provides in-depth coverage of how the transformation of DNA is used to understand the genetic basis behind these fungal traits. 410 0$aFungal Biology,$x2198-7777 606 $aDevelopmental biology 606 $aPlant genetics 606 $aPlant pathology 606 $aPlant breeding 606 $aSystems biology 606 $aDevelopmental Biology$3https://scigraph.springernature.com/ontologies/product-market-codes/L18000 606 $aPlant Genetics and Genomics$3https://scigraph.springernature.com/ontologies/product-market-codes/L32020 606 $aPlant Pathology$3https://scigraph.springernature.com/ontologies/product-market-codes/L24035 606 $aPlant Breeding/Biotechnology$3https://scigraph.springernature.com/ontologies/product-market-codes/L24060 606 $aSystems Biology$3https://scigraph.springernature.com/ontologies/product-market-codes/L15010 615 0$aDevelopmental biology. 615 0$aPlant genetics. 615 0$aPlant pathology. 615 0$aPlant breeding. 615 0$aSystems biology. 615 14$aDevelopmental Biology. 615 24$aPlant Genetics and Genomics. 615 24$aPlant Pathology. 615 24$aPlant Breeding/Biotechnology. 615 24$aSystems Biology. 676 $a570 676 $a571.8 676 $a571.92 676 $a581.35 702 $avan den Berg$b Marco A$4edt$4http://id.loc.gov/vocabulary/relators/edt 702 $aMaruthachalam$b Karunakaran$4edt$4http://id.loc.gov/vocabulary/relators/edt 906 $aBOOK 912 $a9910298282803321 996 $aGenetic Transformation Systems in Fungi, Volume 1$92500505 997 $aUNINA