LEADER 04352nam 22006615 450 001 9910298278703321 005 20200630164526.0 010 $a3-319-13087-0 024 7 $a10.1007/978-3-319-13087-3 035 $a(CKB)3710000000355409 035 $a(EBL)1973891 035 $a(SSID)ssj0001451853 035 $a(PQKBManifestationID)11789694 035 $a(PQKBTitleCode)TC0001451853 035 $a(PQKBWorkID)11479196 035 $a(PQKB)11453254 035 $a(DE-He213)978-3-319-13087-3 035 $a(MiAaPQ)EBC1973891 035 $a(PPN)18449656X 035 $a(EXLCZ)993710000000355409 100 $a20150205d2015 u| 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aMass Spectrometry: Developmental Approaches to Answer Biological Questions /$fby Gwenael Pottiez 205 $a1st ed. 2015. 210 1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2015. 215 $a1 online resource (77 p.) 225 1 $aSpringerBriefs in Bioengineering,$x2193-097X 300 $aDescription based upon print version of record. 311 $a3-319-13086-2 320 $aIncludes bibliographical references. 327 $aGeneral and most usual proteomics methods based on mass spectrometry analysis: Protein identification and protein quantification -- Direct analysis of tissues and body fluids -- Mass spectrometry in archaeology -- Determination and quantification of post-translational modifications -- Protein-Protein interaction determined by mass spectrometry -- Protein structure analysis -- Determination of protein function by mass spectrometry -- Computer-assisted data analysis and data mining for new applications. 330 $aThe understanding of the events taking place in a cell, a biological fluid or in any biological system is the main goal of biology research. Many fields of research use different technology to assess those events. Mass spectrometry is one of those techniques and this undergoes constant evolution and adaptation to always enhance the accuracy of the information provided. Proteomics provides a large panel of data on protein identity and protein expression that were made possible by mass spectrometry. For several years now mass spectrometry has become central to performing proteomic research, however this powerful tool is under constant evolution to be more sensitive and more resolute. More importantly mass spectrometry became a field of research focusing on new applications. Indeed, the complexity in biological systems relies on the changes of expression of transcription of proteins but also on the post-translational modification of proteins, the structure of proteins and the interaction between proteins, amongst others. As of now, several investigations tried to improve the quantification of proteins by mass spectrometry, the determination of post-translational modifications, the protein-protein and protein-nucleic acids interaction or the proteins structures. This book is structured as follows: after a brief introduction of the usual and most popular applications for mass spectrometry in proteomics, the most recent research and developments in mass spectrometry-based methodologies will be explored. 410 0$aSpringerBriefs in Bioengineering,$x2193-097X 606 $aSpectroscopy 606 $aBiochemical engineering 606 $aMedicine 606 $aSpectroscopy/Spectrometry$3https://scigraph.springernature.com/ontologies/product-market-codes/C11020 606 $aBiochemical Engineering$3https://scigraph.springernature.com/ontologies/product-market-codes/C12029 606 $aBiomedicine, general$3https://scigraph.springernature.com/ontologies/product-market-codes/B0000X 615 0$aSpectroscopy. 615 0$aBiochemical engineering. 615 0$aMedicine. 615 14$aSpectroscopy/Spectrometry. 615 24$aBiochemical Engineering. 615 24$aBiomedicine, general. 676 $a54 676 $a543.2-543.8 676 $a610 676 $a660.63 700 $aPottiez$b Gwenael$4aut$4http://id.loc.gov/vocabulary/relators/aut$01062292 906 $aBOOK 912 $a9910298278703321 996 $aMass Spectrometry: Developmental Approaches to Answer Biological Questions$92524195 997 $aUNINA