LEADER 04548nam 22006855 450 001 9910298272203321 005 20200704232654.0 010 $a3-319-12397-1 024 7 $a10.1007/978-3-319-12397-4 035 $a(CKB)3710000000332314 035 $a(EBL)1965274 035 $a(OCoLC)899495715 035 $a(SSID)ssj0001424579 035 $a(PQKBManifestationID)11789944 035 $a(PQKBTitleCode)TC0001424579 035 $a(PQKBWorkID)11368740 035 $a(PQKB)11463005 035 $a(DE-He213)978-3-319-12397-4 035 $a(MiAaPQ)EBC1965274 035 $z(PPN)25886933X 035 $a(PPN)18351730X 035 $a(EXLCZ)993710000000332314 100 $a20150105d2015 u| 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aRecombinant Enzymes - From Basic Science to Commercialization /$fedited by Azura Amid 205 $a1st ed. 2015. 210 1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2015. 215 $a1 online resource (191 p.) 300 $aDescription based upon print version of record. 311 $a3-319-12396-3 320 $aIncludes bibliographical references at the end of each chapters and index. 327 $aIntroduction to Recombinant Enzyme Pre-commercialization -- Recombinant Enzyme: Cloning and Expression -- Common Laboratory Procedure -- Characterization of Recombinant Enzymes -- Purification of Recombinant Protein for Industrial Use -- Recombinant Enzyme Fermentation -- Scaling-up Recombinant Enzyme Fermentation -- Downstream Processing of Recombinant Enzymes for Commercialization -- Economic and Environmental Evaluation of Recombinant Enzyme Production -- Case Study: Recombinant Bromelain Selection -- Case Study: Recombinant Bromelain Cloning, Characterization and Upstream Processes -- Case Study: Recombinant Bromelain Downstream Processing. 330 $aThis edited work presents studies that clarify the basics of producing recombinant enzymes that finally lead to commercialization. It enables researchers to see what is crucial to the commercialization process, from examining the cloning method, using analytical techniques such as calculating the total protein content and enzyme activity, through considering upstream and downstream processes, to the final product.  Readers will discover the importance of the cloning method as it influences the upstream and downstream processes and determines the level of success of the recombinant enzyme commercialization processes. We see that the two main factors that are particularly sensitive during the cloning process are the vector and the host.  A discussion of analytical techniques is presented followed by studies on important stages during the upstream processes including the process of optimizing the media to get results and high enzyme activity. Downstream processes such as the cell disruption technique, purification and formulation of the final product are then considered. The reader is introduced to software that helps streamline recombinant enzyme production from the upstream to downstream processes, to facilitate the process of up-scaling production.  This work includes a case study as tool, to guide understanding of the commercialization process. The work is written for researchers in the field and is especially suited to those who are under pressure to embark on the tough process of commercialization. . 606 $aBiology?Technique 606 $aIndustrial engineering 606 $aProduction engineering 606 $aMedicine 606 $aBiological Techniques$3https://scigraph.springernature.com/ontologies/product-market-codes/L28000 606 $aIndustrial and Production Engineering$3https://scigraph.springernature.com/ontologies/product-market-codes/T22008 606 $aBiomedicine, general$3https://scigraph.springernature.com/ontologies/product-market-codes/B0000X 615 0$aBiology?Technique. 615 0$aIndustrial engineering. 615 0$aProduction engineering. 615 0$aMedicine. 615 14$aBiological Techniques. 615 24$aIndustrial and Production Engineering. 615 24$aBiomedicine, general. 676 $a570 676 $a570.28 676 $a610 676 $a670 702 $aAmid$b Azura$4edt$4http://id.loc.gov/vocabulary/relators/edt 906 $aBOOK 912 $a9910298272203321 996 $aRecombinant Enzymes - From Basic Science to Commercialization$92541193 997 $aUNINA