LEADER 05316nam 2200649 450 001 9910284952203321 005 20180629182913.0 010 $a1-78924-463-3 010 $a1-78064-452-3 035 $a(CKB)3800000000006896 035 $a(EBL)1834013 035 $a(SSID)ssj0001368958 035 $a(PQKBManifestationID)12545629 035 $a(PQKBTitleCode)TC0001368958 035 $a(PQKBWorkID)11289495 035 $a(PQKB)10145939 035 $a(MiAaPQ)EBC1834013 035 $a(PPN)250728931 035 $a(EXLCZ)993800000000006896 100 $a20141121h20142014 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aTransgenic insects $etechniques and applications /$fMark Q. Benedict, editor 210 1$aOxfordshire, England ;$aBoston, Massachusetts :$cCABI,$d2014. 210 4$dİ2014 215 $a1 online resource (401 p.) 225 1 $aCABI Biotechnology Series ;$v3 300 $aDescription based upon print version of record. 311 $a1-78064-451-5 320 $aIncludes bibliographical references at the end of each chapters and index. 327 $aContents; Contributors; Acknowledgements; Preface; PART 1: GERMLINE TRANSFORMATION TECHNOLOGY; 1 Transposons for Insect Transformation; 1.1 Transposable Elements; 1.2 DNA Transposons; 1.3 Transposons with Activity in Insects; 1.4 Summary; References; 2 Transposon-Based Technologies for Insects; 2.1 Transposon-Based Technologies; 2.2 Mutagenesis; 2.3 Germ-Line Transformation; 2.4 Modular Expression Systems; 2.5 Cell/Genetic Ablation; 2.6 Transgene Mis-expression; 2.7 Transgenic Gene Silencing; 2.8 Site-Specifi c Recombination; 2.9 Genetic Sensors; 2.10 Conclusions; References 327 $a3 Sex-, Tissue- and Stage-Specifi c Transgene Expression3.1 Introduction; 3.2 Gene Regulation in Insects; 3.3 Th e Basic Genetic Construct; 3.4 Controlling for Position Eff ects; 3.5 General Considerations for Promoter Choice; 3.6 Sex-Specifi c Transgene Expression; 3.7 Tissue-Specifi c Expression; 3.8 Stage-Specifi c Expression; 3.9 Future Prospects; 3.10 Applications of Sex-, Tissue- and Stage-Specifi c Transgene Expression; References; 4 Docking Systems for Site-Directed Transgene Integration; 4.1 Background and Introduction 327 $a4.2 Site-Specifi c Transgenesis - Generation of Phase 1 Docking Strains4.3 Site-Specifi c Transgenesis - Generation of Phase 2 Integrations; 4.4 Recombinase-Mediated Cassette Exchange; 4.5 Future Developments in the Use of Docking Systems; 4.6 Docking Systems Combined with Transposon Stabilization Strategies; 4.7 Integration of Large, Complex Transgene Constructs; 4.8 Construction of Complex Transgenes by Sequential Use of Integrases; References; 5 Inducible and Repressible Systems for Transgene Expression; 5.1 Introduction; 5.2 Naturally Occurring Systems of Conditional Expression 327 $a5.3 Synthetic Systems5.4 Conclusions; References; 6 Sex Ratio Manipulation for Insect Population Control; 6.1 Introduction; 6.2 Overview of Applications and General Principles; 6.3 Meiotic Drive; 6.4 Sex-Specifi c Lethality; 6.5 Manipulation of Sex Determination Mechanisms; 6.6 Conclusions; References; 7 Conditional Dominant Lethals - RIDL; 7.1 Re-engineering the Sterile Insect Technique; 7.2 Sterile Insects and Genetic Control; 7.3 Engineered Traits; 7.4 Integrated Pest Management; 7.5 Resistance Management; 7.6 Molecular Designs; 7.7 Choosing an Eff ector; 7.8 Choice of Switch 327 $a7.9 Strain Performance7.10 Penetrance; 7.11 Resistance; 7.12 Field Experience and Future Prospects; Notes; Acknowledgements; References; PART 2: APPLICATIONS OF TRANSGENIC INSECTS; 8 Tephritid Fruit Fly Transgenesis and Applications; 8.1 Introduction; 8.2 Transformation with the Minos Vector System; 8.3 Transformation with the piggyBac Vector System; 8.4 Transformation with the Hermes Vector System; 8.5 Marker Systems for Transformant Organismal and Tissue Detection in Tephritid Flies; 8.6 Post-integration Stabilization of Transposon Vectors in Tephritid Flies 327 $a8.7 Site-Specifi c Genomic Targeting in Tephritids 330 $aInsect transgenesis promises improvements in agriculture, pharmaceuticals and public health. Many important insects can now be routinely transformed with effectors that have useful applications. Agriculture presents the largest market for transgenic insects and has a foundational history of success with sterile insect technique for control of pests including Mediterranean fruit flies and screwworms. Biotechnology will contribute superior markers, suppressible sterility and sex-conversion. Public health is also seeing transgenic mosquitoes developed which suppress natural populations and are in 410 0$aCABI biotechnology series ;$v3. 606 $aInsects$xGenetics 606 $aTransgenic animals 606 $aInsect pests$xControl 615 0$aInsects$xGenetics. 615 0$aTransgenic animals. 615 0$aInsect pests$xControl. 676 $a631.5233 702 $aBenedict$b Mark Q. 712 02$aC.A.B. International, 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910284952203321 996 $aTransgenic insects$92793120 997 $aUNINA