LEADER 06422 am 22004693u 450 001 9910258749603321 005 20200520144314.0 010 $a3-11-042998-5 024 7 $a10.1515/9783110429985 035 $a(CKB)3850000000001198 035 $a(DE-B1597)453098 035 $a(OCoLC)1020698102 035 $a(DE-B1597)9783110429985 035 $a(Au-PeEL)EBL4943387 035 $a(CaPaEBR)ebr11500908 035 $a(MiAaPQ)EBC4943387 035 $a(EXLCZ)993850000000001198 100 $a20180210h20182018 uy 0 101 0 $aeng 135 $aurcnu|||||||| 181 $2rdacontent 182 $2rdamedia 183 $2rdacarrier 200 00$aMultiphoton microscopy and fluorescence lifetime imaging $eapplications in biology and medicine /$fedited by Karsten Ko?nig 210 1$aBerlin, [Germany] ;$aBoston, [Massachusetts] :$cDe Gruyter,$d2018. 210 4$d©2018 215 $a1 online resource (450 pages) 311 $a3-11-043898-4 320 $aIncludes bibliographical references at the end of each chapters and index. 327 $tFrontmatter -- $tPreface -- $tForeword -- $tContents -- $tList of contributing authors -- $tPart I: Basics -- $t1 Brief history of fluorescence lifetime imaging / $rKönig, Karsten -- $t2 The long journey to the laser and its use for nonlinear optics / $rKaiser, Wolfgang -- $t3 Advanced TCSPC-FLIM techniques / $rBecker, Wolfgang -- $t4 Ultrafast lasers in biophotonics / $rKrueger, Arnd -- $tPart II: Modern nonlinear microscopy of live cells -- $t5 STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities / $rLanzano, Luca / Vicidomini, Giuseppe / Scipioni, Lorenzo / Castello, Marco / Diaspro, Alberto -- $t6 Principles and applications of temporal-focusing wide-field two-photon microscopy / $rSo, Peter T. C. / Choi, Heejin / Yew, Elijah / Rowlands, Christopher -- $t7 FLIM-FRET microscopy / $rAlam, Shagufta Rehman / Melia, Meghan J.O / Wallrabe, Horst / Svindrych, Zdenek / Chandra, Dhyan / Joshi, Suchitra / Kapur, Jaideep / Periasamy, Ammasi -- $t8 TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing / $rRück, Angelika / Breymayer, Jasmin / Kalinina, Sviatlana -- $t9 Laser tweezers are sources of two-photon effects / $rKönig, Karsten -- $t10 Metabolic shifts in cell proliferation and differentiation / $rShirmanova, Marina / Sergeeva, Tatiana / Druzhkova, Irina / Meleshina, Aleksandra / Lukina, Maria / Dudenkova, Varvara / Shcheslavskiy, Vladislav / Becker, Wolfgang / Belousov, Vsevolod / Mishina, Nataliya / Zagaynova, Elena -- $t11 Femtosecond laser nanoprocessing / $rKönig, Karsten -- $t12 Cryomultiphoton imaging / $rBreunig, Hans Georg / König, Karsten -- $tPart III: Nonlinear tissue imaging -- $t13 Multiphoton Tomography (MPT) / $rKönig, Karsten -- $t14 Clinical multimodal CARS imaging / $rWeinigel, Martin / Breunig, Hans Georg / König, Karsten -- $t15 In vivo multiphoton microscopy of human skin / $rBalu, Mihaela / Kelly, Kristen M. / Harris, Ronald M. / König, Karsten / Zachary, Christopher B. / Tromberg, Bruce J. -- $t16 Two-photon microscopy and fluorescence lifetime imaging of the cornea / $rBatista, Ana / Breunig, Hans Georg / Donitzky, Christoph / König, Karsten -- $t17 Multiscale correlative imaging of the brain / $rMascaro, Anna Letizia Allegra / Silvestri, Ludovico / Sacconi, Leonardo / Pavone, Francesco S. -- $t18 Revealing interaction of dyes and nanomaterials by multiphoton imaging / $rHolmes, Amy / Thorling, Camilla / Liu, Xin / Liang, Xiaowen / Wang, Haolu / Breunig, Hans G. / König, Karsten / Studier, Hauke / Roberts, Michael S. -- $t19 Multiphoton FLIM in cosmetic clinical research / $rPena, Ana-Maria / Decencière, Etienne / Brizion, Sébastien / Victorin, Steeve / Koudoro, Serge / Baldeweck, Thérèse / Tancrède-Bohin, Emmanuelle -- $t20 Multiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgery / $rKantelhardt, Sven R. -- $t21 Non-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse models / $rUchugonova, Aisada / Hoffman, Robert M. -- $t22 Bedside assessment of multiphoton tomography / $rMess, Christian / Huck, Volker -- $tIndex 330 $aThis monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard. Contents Part I: Basics Brief history of fluorescence lifetime imaging The long journey to the laser and its use for nonlinear optics Advanced TCSPC-FLIM techniques Ultrafast lasers in biophotonics Part II: Modern nonlinear microscopy of live cells STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities Principles and applications of temporal-focusing wide-field two-photon microscopy FLIM-FRET microscopy TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing Laser tweezers are sources of two-photon effects Metabolic shifts in cell proliferation and differentiation Femtosecond laser nanoprocessing Cryomultiphoton imaging Part III: Nonlinear tissue imaging Multiphoton Tomography (MPT) Clinical multimodal CARS imaging In vivo multiphoton microscopy of human skin Two-photon microscopy and fluorescence lifetime imaging of the cornea Multiscale correlative imaging of the brain Revealing interaction of dyes and nanomaterials by multiphoton imaging Multiphoton FLIM in cosmetic clinical research Multiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgery Non-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse models Bedside assessment of multiphoton tomography 606 $aFluorescence microscopy 615 0$aFluorescence microscopy. 676 $a616.07/58 702 $aKo?nig$b Karsten 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910258749603321 996 $aMultiphoton microscopy and fluorescence lifetime imaging$92259968 997 $aUNINA