LEADER 05183nam 22007935 450 001 9910254249803321 005 20251116145613.0 010 $a3-662-49118-4 024 7 $a10.1007/978-3-662-49118-8 035 $a(CKB)3710000000577118 035 $a(EBL)4354111 035 $a(SSID)ssj0001606945 035 $a(PQKBManifestationID)16316129 035 $a(PQKBTitleCode)TC0001606945 035 $a(PQKBWorkID)14896941 035 $a(PQKB)10039459 035 $a(DE-He213)978-3-662-49118-8 035 $a(MiAaPQ)EBC4354111 035 $a(PPN)191701424 035 $a(EXLCZ)993710000000577118 100 $a20160121d2016 u| 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aEssentials of Single-Cell Analysis $eConcepts, Applications and Future Prospects /$fedited by Fan-Gang Tseng, Tuhin Subhra Santra 205 $a1st ed. 2016. 210 1$aBerlin, Heidelberg :$cSpringer Berlin Heidelberg :$cImprint: Springer,$d2016. 215 $a1 online resource (415 p.) 225 1 $aSeries in BioEngineering,$x2196-8861 300 $aDescription based upon print version of record. 311 08$a3-662-49116-8 320 $aIncludes bibliographical references. 327 $aSingle Cell Behavioral Assays for Heterogeneity Studies -- Systems biology in single cells -- Electroporation for single cell analysis -- Microinjection for single cell analysis -- Optical tools for single cell manipulation and analysis -- Optoelectrokinetic manipulation for cell analysis -- Continuous micro/nano-fluidic devices for single cell analysis -- Single Cell Mechanical Properties: Label-Free Biomarkers for Cell Status Evaluation -- Cytometry of single cells for biology and biomedicine -- Single cell genomics and epigenomics -- Single cell Metabolomics -- Applications of cell based drug delivery systems: use of single cell assay -- Applications of Single Cell Sequencing in Cancer -- Single Cell Characterization of Microalgal Lipid Contents with Confocal Raman Microscopy -- Single Differentiated Neurons from Pluripotent Embryonic Stem Cells: Motor Protein Modelling and Neurodegenerative Disease. 330 $aThis book provides an overview of single-cell isolation, separation, injection, lysis and dynamics analysis as well as a study of their heterogeneity using different miniaturized devices. As an important part of single-cell analysis, different techniques including electroporation, microinjection, optical trapping, optoporation, rapid electrokinetic patterning and optoelectronic tweezers are described in detail. It presents different fluidic systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry) and their integration with sensor technology, optical and hydrodynamic stretchers etc., and demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems. It also discusses the future challenges for single-cell analysis, including the advantages and limitations. This book is enjoyable reading material while at the same time providing essential information to scientists in academia and professionals in industry working on different aspects of single-cell analysis. Dr. Fan-Gang Tseng is a Distinguished Professor of Engineering and System Science at the National Tsing Hua University, Taiwan. Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA. 410 0$aSeries in BioEngineering,$x2196-8861 606 $aBiomedical engineering 606 $aBiotechnology 606 $aBioinformatics 606 $aBiophysics 606 $aBiophysics 606 $aCytology 606 $aBiomedical Engineering and Bioengineering$3https://scigraph.springernature.com/ontologies/product-market-codes/T2700X 606 $aMicroengineering$3https://scigraph.springernature.com/ontologies/product-market-codes/C12040 606 $aBioinformatics$3https://scigraph.springernature.com/ontologies/product-market-codes/L15001 606 $aBiological and Medical Physics, Biophysics$3https://scigraph.springernature.com/ontologies/product-market-codes/P27008 606 $aCell Biology$3https://scigraph.springernature.com/ontologies/product-market-codes/L16008 615 0$aBiomedical engineering. 615 0$aBiotechnology. 615 0$aBioinformatics. 615 0$aBiophysics. 615 0$aBiophysics. 615 0$aCytology. 615 14$aBiomedical Engineering and Bioengineering. 615 24$aMicroengineering. 615 24$aBioinformatics. 615 24$aBiological and Medical Physics, Biophysics. 615 24$aCell Biology. 676 $a571.6028 702 $aTseng$b Fan-Gang$4edt$4http://id.loc.gov/vocabulary/relators/edt 702 $aSantra$b Tuhin Subhra$4edt$4http://id.loc.gov/vocabulary/relators/edt 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910254249803321 996 $aEssentials of Single-Cell Analysis$91544983 997 $aUNINA