LEADER 04107nam 22006615 450 001 9910254147703321 005 20200706010546.0 010 $a9784431565154 010 $a9784431565178 (e-book) 024 7 $a10.1007/978-4-431-56517-8 035 $a(CKB)3710000001095349 035 $a(DE-He213)978-4-431-56517-8 035 $a(MiAaPQ)EBC4821146 035 $a(PPN)199764956 035 $a(EXLCZ)993710000001095349 100 $a20170309d2017 u| 0 101 0 $aeng 135 $aurnn|008mamaa 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aApplications of Aminoacylation Ribozymes That Recognize the 3?-end of tRNA /$fby Naohiro Terasaka 205 $a1st ed. 2017. 210 1$aTokyo :$cSpringer Japan :$cImprint: Springer,$d2017. 215 $a1 online resource (XII, 89 p. 38 illus., 28 illus. in color.) 225 1 $aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053 300 $a"Doctoral Thesis accepted by the University of Tokyo, Tokyo, Japan." 311 $a4-431-56515-9 311 $a4-431-56517-5 320 $aIncludes bibliographical references. 327 $aGeneral introduction -- Discovery of human microRNA precursor binding to folic acid by small RNA transcriptomic SELEX -- Orthogonal ribosome-tRNAs pair by engineering of peptidyl transferase center -- General conclusion. 330 $aIn this thesis, applications of aminoacylation ribozymes named flexizymes are described. Flexizymes have the following unique characteristics: (i) substrate RNA is recognized by two consecutive base pairs between the 3'-end of substrate RNA and the 3'-end of the flexizyme; (ii) these base pairs can be substituted with other base pairs; and (iii) various activated amino acids can be used as substrates including both canonical and noncanonical amino acids. This flexible aminoacylation of RNAs by flexizymes was used to label endogenous tRNAs to be removed, and in vitro selection using the tRNA-depleted library enabled the discovery of the novel interaction between the microRNA precursor and metabolites. Flexizymes are also used to prepare various aminoacyl-tRNAs bearing mutations at the 3'-end to engineer the translation machinery and to develop the orthogonal translation machinery. The first part of the research demonstrated that SELEX is appropriate for discovering the interaction between small RNA and ligands, and suggested that more RNA motif binding to small molecules exists in small RNAs. The second part opened a door to new opportunities for in vitro synthetic biology involving the engineering of the genetic codes and translation machineries. This research also indicated the great potential of aminoacylation by flexizymes to be applied in various fields of RNA research, which is beneficial for RNA researchers. 410 0$aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053 606 $aBioorganic chemistry 606 $aCatalysis 606 $aNucleic acids 606 $aBiotechnology 606 $aBioorganic Chemistry$3https://scigraph.springernature.com/ontologies/product-market-codes/C19010 606 $aCatalysis$3https://scigraph.springernature.com/ontologies/product-market-codes/C29000 606 $aNucleic Acid Chemistry$3https://scigraph.springernature.com/ontologies/product-market-codes/L14011 606 $aBiotechnology$3https://scigraph.springernature.com/ontologies/product-market-codes/C12002 615 0$aBioorganic chemistry. 615 0$aCatalysis. 615 0$aNucleic acids. 615 0$aBiotechnology. 615 14$aBioorganic Chemistry. 615 24$aCatalysis. 615 24$aNucleic Acid Chemistry. 615 24$aBiotechnology. 676 $a540 700 $aTerasaka$b Naohiro$4aut$4http://id.loc.gov/vocabulary/relators/aut$0766768 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910254147703321 996 $aApplications of Aminoacylation Ribozymes That Recognize the 3?-end of tRNA$91560463 997 $aUNINA