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024 7 $a10.1007/978-3-319-03236-8
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100   $a20151016d2016      u|         0
101 0 $aeng
135   $aur|n|---|||||
181   $ctxt
182   $cc
183   $acr
200 12$aA Novel Heme-Thiolate Peroxygenase AaeAPO and Its Implications for C-H Activation Chemistry /$fby Xiaoshi Wang
205   $a1st ed. 2016.
210  1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2016.
215   $a1 online resource (153 p.)
225 1 $aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053
300   $aDescription based upon print version of record.
311   $a3-319-03235-6 
320   $aIncludes bibliographical references at the end of each chapters.
327   $aFrom the Contents: Hydrocarbon Oxygenation by Heme-Thiolate Enzymes -- Efficient and Selective Alkane Hydroxylation Reactions Catalyzed by the Fungal Peroxygenase AaeAPO -- Hydrocarbon Hydroxylations Catalyzed by AaeAPO: Evidence of Radical Intermediates and Kinetic Isotope Effects.
330   $aIn this thesis, Xiaoshi Wang investigates the function and mechanism of a newly discovered heme-thiolate peroxygenase, AaeAPO. This enzyme class comes from Agrocybe aegerita and is used in the conversion of inert hydrocarbons to alcohols. Xiaoshi's work focuses on an extracellular P450 enzyme which is not limited in its stability and lack of solubility and therefore is relevant for widespread industrial use. The author demonstrates that the peroxygenase catalyzes a wide range of reactions. In some cases the author even describes very difficult transformations in molecules that are highly inert. Her detailed investigations provide a mechanistic framework for how the peroxygenase catalyzes such a large number of reactions. A major highlight of this thesis is the identification of key short-lived intermediates in the catalytic cycle of the peroxygenase, using rapid kinetic and spectroscopic methods, as well as the elucidation of the thermodynamic properties of these high-energy intermediates. This work adds new insight into an important class of enzymes.
410  0$aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053
606   $aChemistry, Organic
606   $aEnzymology
606   $aCatalysis
606   $aOrganic Chemistry$3https://scigraph.springernature.com/ontologies/product-market-codes/C19007
606   $aEnzymology$3https://scigraph.springernature.com/ontologies/product-market-codes/L14070
606   $aCatalysis$3https://scigraph.springernature.com/ontologies/product-market-codes/C29000
615  0$aChemistry, Organic.
615  0$aEnzymology.
615  0$aCatalysis.
615 14$aOrganic Chemistry.
615 24$aEnzymology.
615 24$aCatalysis.
676   $a612.1111
700   $aWang$b Xiaoshi$4aut$4http://id.loc.gov/vocabulary/relators/aut$01061405
801  0$bMiAaPQ
801  1$bMiAaPQ
801  2$bMiAaPQ
906   $aBOOK
912   $a9910254057303321
996   $aA Novel Heme-Thiolate Peroxygenase AaeAPO and Its Implications for C-H Activation Chemistry$92518743
997   $aUNINA