LEADER 06020nam 22006615 450 001 9910254053303321 005 20190525051657.0 010 $a3-319-26070-7 024 7 $a10.1007/978-3-319-26070-9 035 $a(CKB)3710000000595328 035 $a(EBL)4334064 035 $a(SSID)ssj0001606894 035 $a(PQKBManifestationID)16317006 035 $a(PQKBTitleCode)TC0001606894 035 $a(PQKBWorkID)14897157 035 $a(PQKB)11726264 035 $a(DE-He213)978-3-319-26070-9 035 $a(MiAaPQ)EBC4334064 035 $a(PPN)191700495 035 $a(EXLCZ)993710000000595328 100 $a20160112d2016 u| 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aApplications of Mass Spectrometry in Microbiology $eFrom Strain Characterization to Rapid Screening for Antibiotic Resistance /$fedited by Plamen Demirev, Todd R. Sandrin 205 $a1st ed. 2016. 210 1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2016. 215 $a1 online resource (336 p.) 300 $aDescription based upon print version of record. 311 $a3-319-26068-5 320 $aIncludes bibliographical references at the end of each chapters and index. 327 $aIntroduction -- SECTION I. METHODOLOGY AND TECHNIQUES.-  Methods and instrumentation in mass spectrometry for the differentiation of closely related microorganisms.- Sample preparation methods for the rapid MS analysis of microorganisms.-  Advantages offered by proteomic strategies for rapid biodetection.- Bottom Up Proteomics Methods for Strain-Level Typing and Identification of Bacteria.- Maximizing the taxonomic resolution of MALDI-TOF-MS-based approaches to bacterial characterization:  From culture conditions through data analysis -- SECTION II. SUB-SPECIES DISCRIMINATION.-   Modulation of the Discriminatory Power of MALDI-TOF MS Profiling for Distinguishing between Closely Related Bacterial Strains.-  Discriminatory Power of MALDI-TOF Mass Spectrometry for Phylogenetically Closely Related Microbial Strains.- MALDI-TOF MS as a novel tool for dereplication and characterization of microbiota in bacterial diversity studies.- Bacterial Identification at the Serovar level by Top-down Mass Spectrometry -- SECTION III. DRUG RESISTANCE MONITORING AND ASSAYS.- Rapid profiling of human pathogenic bacteria and antibiotic resistance employing specific tryptic peptides as biomarkers.- Detection of ?-lactamases and their activity using MALDI-TOF MS.- Stable-isotope based strategies for rapid determination of drug resistance by mass spectrometry. 330 $aIn the last quarter century, advances in mass spectrometry (MS) have been at the forefront of efforts to map complex biological systems including the human metabolome, proteome, and microbiome. All of these developments have allowed MS to become a well-established molecular level technology for microbial characterization. MS has demonstrated its considerable advantage as a rapid, accurate, and cost-effective method for microbial identification, compared to conventional phenotypic techniques. In the last several years, applications of MS for microbial characterization in research, clinical microbiology, counter-bioterrorism, food safety, and environmental monitoring have been documented in thousands of publications. Regulatory bodies in Europe, the US, and elsewhere have approved MS-based assays for infectious disease diagnostics. As of mid-2015, more than 3300 commercial MS systems for microbial identification have been deployed worldwide in hospitals and clinical labs. While previous work has covered broader approaches in using MS to characterize microorganisms at the species level or above, this book focuses on strain-level and subtyping applications. In thirteen individual chapters, innovators, leaders and practitioners in the field from around the world have contributed to a comprehensive overview of current and next-generation approaches for MS-based microbial characterization at the subspecies and strain levels. Chapters include up-to-date reference lists as well as web-links to databases, recommended software, and other useful tools. The emergence of new, antibiotic-resistant strains of human or animal pathogens is of extraordinary concern not only to the scientific and medical communities, but to the general public as well. Developments of novel MS-based assays for rapid identification of strains of antibiotic-resistant microorganisms are reviewed in the book as well. Microbiologists, bioanalytical scientists, infectious disease specialists, clinical laboratory and public health practitioners as well as researchers in universities, hospitals, government labs, and the pharmaceutical and biotechnology industries will find this book to be a timely and valuable resource. 606 $aMass spectrometry 606 $aMicrobiology 606 $aEpidemiology 606 $aDrug interactions 606 $aMass Spectrometry$3http://scigraph.springernature.com/things/product-market-codes/C11010 606 $aApplied Microbiology$3http://scigraph.springernature.com/things/product-market-codes/C12010 606 $aEpidemiology$3http://scigraph.springernature.com/things/product-market-codes/H63000 606 $aDrug Resistance$3http://scigraph.springernature.com/things/product-market-codes/B16020 615 0$aMass spectrometry. 615 0$aMicrobiology. 615 0$aEpidemiology. 615 0$aDrug interactions. 615 14$aMass Spectrometry. 615 24$aApplied Microbiology. 615 24$aEpidemiology. 615 24$aDrug Resistance. 676 $a540 702 $aDemirev$b Plamen$4edt$4http://id.loc.gov/vocabulary/relators/edt 702 $aSandrin$b Todd R$4edt$4http://id.loc.gov/vocabulary/relators/edt 906 $aBOOK 912 $a9910254053303321 996 $aApplications of Mass Spectrometry in Microbiology$92508540 997 $aUNINA