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101 0 $aeng
135   $aur|n|---|||||
181   $ctxt
182   $cc
183   $acr
200 10$aUsing Mass Spectrometry for Biochemical Studies on Enzymatic Domains from Polyketide Synthases /$fby Matthew Jenner
205   $a1st ed. 2016.
210  1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2016.
215   $a1 online resource (189 p.)
225 1 $aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053
300   $aDescription based upon print version of record.
311   $a3-319-32722-4 
320   $aIncludes bibliographical references at the end of each chapters.
327   $aIntroduction -- Materials and Methods -- Substrate Specificity of Ketosynthase Domains Part I: ?-Branched Acyl Chains -- Substrate Specificity of Ketosynthase Domains Part II: Amino Acid-Containing Acyl Chains -- Synthesis of Acyl-Acyl Carrier Proteins and their use in Studying Polyketide Synthase Enzymology -- Substrate Specificity of Ketosynthase Domains Part III: Elongation-Based Substrate Specificity.
330   $aThis thesis reports studies on the substrate specificity of crucial ketosynthase (KS) domains from trans-AT Polyketide Synthases (PKSs). Using a combination of electrospray ionisation-mass spectrometry (ESI-MS) and simple N-acetyl cysteamine (SNAC) substrate mimics, Matthew Jenner has successfully studied the specificity of a range of KS domains from the bacillaene and psymberin PKSs with regard to the initial acylation step of KS-catalysis. The findings in this thesis provide important insights into mechanisms of KS specificity and show that mutagenesis can be used to expand the repertoire of acceptable substrates for future PKS engineering. The documentation of this research is a useful reference and guideline for students starting a PhD in this field.
410  0$aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053
606   $aMass spectrometry
606   $aEnzymology
606   $aBiochemical engineering
606   $aMedical biochemistry
606   $aMass Spectrometry$3https://scigraph.springernature.com/ontologies/product-market-codes/C11010
606   $aEnzymology$3https://scigraph.springernature.com/ontologies/product-market-codes/L14070
606   $aBiochemical Engineering$3https://scigraph.springernature.com/ontologies/product-market-codes/C12029
606   $aMedical Biochemistry$3https://scigraph.springernature.com/ontologies/product-market-codes/H35005
615  0$aMass spectrometry.
615  0$aEnzymology.
615  0$aBiochemical engineering.
615  0$aMedical biochemistry.
615 14$aMass Spectrometry.
615 24$aEnzymology.
615 24$aBiochemical Engineering.
615 24$aMedical Biochemistry.
676   $a572.45
700   $aJenner$b Matthew$4aut$4http://id.loc.gov/vocabulary/relators/aut$01059892
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801  1$bMiAaPQ
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906   $aBOOK
912   $a9910254029703321
996   $aUsing Mass Spectrometry for Biochemical Studies on Enzymatic Domains from Polyketide Synthases$92509383
997   $aUNINA